Editor’s Choice Articles

This study examines how rural development in China shapes new trends in population migration. Using first-hand, village-level data from Zhejiang—an economically developed province in China—we investigated the patterns and influencing factors of population migration between rural and urban areas. We conceptualized three types of migration in rural areas: rural out-migration, rural in-migration, and rural return-migration. First-hand data were collected from 347 villages. The results show that although rural out-migration remains the dominant form of migration, rural in-migration and return-migration are also common, and the latter two are positively correlated. Further, we found evidence to support the conclusion that rural economic, social, and spatial development encourages rural in-migration and return-migration but does not have a significant impact on rural out-migration. Therefore, it is foreseeable that rural in-migration and return-migration will become increasingly common in China. Full article

Natural disasters cause great losses of property and life in many areas of China. However, rural residents do not always insure themselves against these losses. Measuring the correlation between trust and farmers’ behavior related to the purchasing of natural disaster insurance is of great significance to the implementation of natural disaster insurance pilot programs and insurance systems in China. This article analyzes data from a survey of 327 households in four districts and counties of Sichuan Province, China, that were affected by the Wenchuan and Lushan earthquakes. According to the relevant theories of trust, trust was divided into three dimensions: authority trust, collective trust, and relationship trust. Then a technology acceptance model was built, and PLS-SEM was used to comprehensively analyze the correlation between different dimensions of trust and farmers’ insurance purchase behavior. The results show that (1) only relationship trust was directly and significantly positively correlated with insurance purchasing behavior. Although there was no direct significant correlation between authoritative trust or collective trust and buying behavior, relationship trust was found to indirectly affect buying behavior. (2) Younger farmers and those with higher incomes are more likely to buy disaster insurance if they live in a disaster-threat zone, have experienced disasters, and are risk averse. We then discuss the correlations between farmers’ trust and natural disaster insurance purchasing in areas threatened by earthquake disasters. This provides a policy inspiration for the promotion of disaster insurance and the construction of insurance systems in China. Full article

We investigated whether juglone is the only allelochemical in a leaf extract from the walnut (Juglans regia L.). This was achieved through comparisons of the effects of pure juglone (1 mM, 100 μM, 10 μM control juglone) and J. regia leaf extract (prepared as 1 mM, 100 μM leaf juglone) on seed germination, seedling growth, and secondary metabolism of the selected crop vegetables. Two control treatments were also applied, as extraction medium and water. For inhibition of seed germination, S. lycopersicum, B. rapa var. japonica, and V. locusta were more sensitive to 1 mM leaf juglone, and L. sativa was more sensitive to 1 mM control juglone. This suggests that this walnut leaf extract contains specific phenolic substance(s) that can stimulate seed germination in some species and inhibit it in others. Seedling length was more sensitive to 1 mM leaf juglone than 1 mM control juglone, with selective strong inhibition of root length versus shoot length by 1 mM control juglone. Juglone also had significant effects on the secondary metabolism of L. sativa, in particular for seedlings treated with 100 μM control juglone, with marked decreases in all secondary metabolites studied. Flavonols constituted the majority of these metabolites in L. culinaris, which showed the least sensitivity to both control juglone and leaf juglone treatments. Thirty compounds were identified and quantified in S. lycopersicum, L. culinaris, and L. sativa, some for the first time in these plants, and all for the first time in the seedlings of these crop vegetables. Full article

The main goal of the current study was to investigate the effects of feeding low protein (LP) diets on the performance parameters and excreta composition of broiler chickens. In total, 288 male Ross 308 day-old chickens were divided into two dietary treatment groups using six replicate pens with 24 chickens each. No LP diet was fed in the starter phase. The protein reduction in the grower and finisher phases were 1.8% and 2% respectively. Beside the measurements of production traits, on day 24 and 40 representative fresh excreta samples were collected, their dry matter, total N, NH₄⁺-N and uric acid-N contents determined, and the ratio of urinary and fecal N calculated. Dietary treatments failed to cause significant differences in the feed intake, growth rate, and feed conversion ratio of animals. LP diets decreased the total nitrogen and uric acid contents of excreta significantly. The age of birds had also significant effect, resulting more reduction in the grower phase compared with the finisher. The ratio of urinary N was higher at day 40 compared with the age of day 24. The urinary N content of broiler chicken’s excreta is lower than can be found in the literature, which should be considered in the ammonia inventory calculations. Full article

After the completion of China’s poverty alleviation task in 2020, the poverty situation will undergo major changes, and the focus of poverty alleviation work will shift to solving relative poverty. This can provide useful inspiration for the government to formulate and implement relevant policies that explore the differences in livelihood risk impacts of different types of farmers in areas where disasters and poverty are intertwined. This study used survey data of 327 households in four districts and counties in the hardest-hit areas of the Wenchuan Earthquake and Lushan Earthquake in China’s Sichuan Province in 2018. This study measured farmers’ livelihood risks from four aspects: health risks, environmental risks, financial risks, and social risks, and measured farmers’ poverty types from three aspects: absolutely poor farmers, relatively poor farmers, and non-poor farmers. It systematically analyzed the four types of livelihood risks faced by farmers and the three types of poverty they were in, and constructed a multinomial logistic regression to explore the correlation between livelihood risks and poverty types. This study is the first to compare the relatively poor type with other poverty types. The second innovation is that it uses the entropy method and multinomial logistic regression. The results showed that: (1) Among the four livelihood risks faced by farmers, the biggest was the environmental risk, the next was financial risk, the third was health risk, and the last was social risk. (2) Among the three poverty types, farmers in absolute poverty were the most populated, with non-poor farmers next, and relatively poor farmers at the bottom. (3) Farmers of different poverty types were affected by livelihood risks to different degrees. Specifically, when compared with the impacts on farmers in absolute poverty, the impacts social risks could bring to farmers in relative poverty were more severe while the impacts of health risks, environmental risks and financial were not that strong. Impacted by social risks, relatively poor farmers are more seriously impacted by public affairs and social security status. When compared with the impacts on non-poor farmers, the impacts of all these four risks on relatively poor farmers were not notable. Full article

Shading or low light (LL) conditions are a key and necessary cultivation technique in cigar wrapper tobacco production. However, the effect of low light on the photosynthesis in cigar tobacco is not clear. Therefore, this study is designed to know the photosynthesis of cigar tobacco under different light intensities (T200, T100, and T50 μmol m⁻² s⁻¹). The results reveal that under low light, T50 especially improved the light interception and increased carbon utilization, as witnessed by a higher specific leaf area and lower specific leaf weight. Low light intensity caused better light interception and carbon utilization in cigar tobacco leaves, and thus thinner leaves are more able to use low light efficiently. The chlorophyll content is related to the photosynthesis process; thus, LL affected the photosynthesis process by lowering the chlorophyll content. Similarly, LL also altered the photosynthetic efficiency by lowering the QY_Lss, qP_Lss, and Rfd_Lss. Additionally, higher expression of Lhcb4.2, Lhcb6, PsbA, PsbB, and PsbD under low light, especially T50, shows that the PSII and antenna proteins complex efficiently utilized the absorbed energy for photosynthesis. Finally, the lower photosynthesis, particularly in T50, is attributed to the downregulation of genes related to NADPH production (petH) and the rubisco enzyme synthesis-related gene (rbcs) for CO₂ fixation in the Calvin cycle. Overall, the results show that the photosynthesis is decreased under LL intensities which might be related to lower chlorophyll content and downregulation of petH and rbcs genes. Full article

Orchard pesticide off-target deposition and drift cause substantial soil and water pollution, and other environmental pollution. Orchard target-oriented spraying technologies have been used to reduce the deposition and drift caused by off-target spraying and control environmental pollution to within an acceptable range. Two target-oriented spraying systems based on photoelectric sensors or ultrasonic sensors were developed. Three spraying treatments of young cherry trees and adult apple trees were conducted using a commercial sprayer with a photoelectric-based target-oriented spraying system, an ultrasonic-based target-oriented spraying system or no target-oriented spraying system. A rhodamine tracer was used instead of pesticide. Filter papers were fixed in the trees and on the ground. The tracer on the filter papers was washed off to calculate the deposition distribution in the trees and on the ground. The deposition data were used to evaluate the systems and pesticide off-target deposition achieved with orchard target-oriented sprayers. The results showed that the two target-oriented spraying systems greatly reduced the ground deposition compared to that caused by off-target spraying. Compared with that from off-target spraying, the ground deposition from photoelectric-based (trunk-based) and ultrasonic-based (canopy-based) target-oriented spraying decreased by 50.63% and 38.74%, respectively, for the young fruit trees and by 21.66% and 29.87%, respectively, for the adult fruit trees. The trunk-based target-oriented detection method can be considered more suitable for young trees, whereas the canopy-based target-oriented detection method can be considered more suitable for adult trees. The maximum ground deposition occurred 1.5 m from the tree trunk at the back of the tree canopy and was caused by the high airflow at the air outlet of the sprayer. A suitable air speed and air volume at the air outlet of the sprayer can reduce pesticide deposition on the ground. Full article

Rice is a staple food for more than half of the global population. Rice production is, however, severely affected by biotic and abiotic stresses. Fertilisers and pesticides are widely used in rice farming to maintain optimal yield and to prevent losses caused by environmental stress. However, the indiscriminate use of agrochemicals has adverse effects on the environment and human health. Stress caused by nutrient excess or deficiency has an impact on plant disease resistance. The interference of plant responses induced by nutrient stress can result in a positive or negative impact on resistance to pathogen infection. In this review, we explore the effects of combined stresses in rice, focusing on nutrient stress, such as nitrogen and phosphorous supply, and infection by fungal pathogen Magnaporthe oryzae, the causal agent of rice blast disease. Crosstalk between nutrient stress-induced and pathogen-induced signalling pathways in rice is discussed, as well, with particular emphasis on regulatory microRNAs. Understanding the interconnected regulations between nutrient stress and disease resistance will lay a foundation for rationally optimising fertiliser and pesticide use in rice production. Full article

African swine fever (ASF) in Poland has been a major concern for pig production for the last seven years. The main constrains in ASF eradication in Poland are due to the high density of wild boar in ASF-affected areas of the country. Other factors, responsible for long-distance ASF spread to new regions of the country, are mainly related to human-mediated activity and lack of awareness of the potential disease threat to pig production. In the case of pig holdings, the only effective measure of ASF prevention is strict implementation of biosecurity rules. Despite many efforts to implement these measures, the disease occurred in a number of voivodeships, poviats and counties of Poland, primarily in eastern Poland; however, starting from 2019, also in the western part of the country. Further recommendations to eradicate ASF in Poland or at least to minimize the economic loss caused by ASF in pig production include effective wild boar population management strategies along with the implementation of strict biosecurity measures. The observations from the last seven years of ASF epizootic in Poland clearly indicate that the disease could not have been effectively controlled in wild boar population and could only be restricted in domestic pig population following severe biosecurity rules. As for ASF spread control in wild boar population, the measures applied in other EU countries include active wild boar carcass search and disposal along with sanitary and reduction hunting. These measures have also been shown non-effective. The only solution for future sustainable pig production in Poland seems to be strict collaboration between pig producers, veterinary inspection, and hunting associations. Full article

Conservation agriculture (CA) is considered a sustainable practice with the potential to maintain or increase crop productivity and improve environmental quality and ecosystem services. It typically improves soil quality and water conservation; however, its effect on crop productivity is highly variable and dependent on local conditions/management. Crop residue retention plays a crucial role in CA and can help to improve overall soil health and ultimately crop productivity and sustainability. However, weed control, herbicide resistance, and weed shift under residue retained fields is a major challenge. Moreover, CA can increase water infiltration and reduce soil loss and runoff. This reduces the surface transport of nitrate and phosphorus from agricultural fields and the eutrophication of water bodies, although leaching of nitrate to groundwater can potentially increase. In addition, CA has been proposed as one of the components in climate-smart agriculture, owing to its reduced period to seed/plant next crop, reduced soil disturbance and low consumption of fossil fuels. Therefore, compared to the conventional intensive tillage, CA has a greater potential for soil C sequestration, favors higher soil biodiversity, lowers greenhouse gas emission, and can assist in mitigating climate change. However, not all experiments report a positive impact. The understanding and decoding the site-specific complexities of CA system is important and requires a multidisciplinary approach. Full article

Rice is one of the most widely consumed cereals in the world. The husks of harvested, unprocessed rice are not digested by humans and need to be removed to obtain edible grains, whereas the bran can be partially (brown rice) or totally removed (white rice). Brown rice is a wholegrain cereal and, as such, is known to have beneficial effects on human health. Recent epidemiological studies have shown that the consumption of whole grains can reduce the risk of metabolic disorders, cardiovascular diseases, and some types of cancer. However, white rice is preferred for reasons connected to appearance, taste, palatability, ease of cooking, tradition, safety, shelf-life, and lack of awareness about its benefits and availability. In this review, the latest scientific reports regarding the nutritional composition of brown rice and the evolution of the technology for its production will be briefly reviewed together with research on nutritional implications of brown rice consumption also in relation to cancer development in humans. A specific chapter is devoted to pigmented rice which, thanks to its composition, has attracted the growing interest of consumers worldwide. The need for further studies to help promote the consumption of wholegrain rice are also discussed. Full article

Crop production can be greatly reduced due to various diseases, which seriously endangers food security. Thus, detecting plant diseases accurately is necessary and urgent. Traditional classification methods, such as naked-eye observation and laboratory tests, have many limitations, such as being time consuming and subjective. Currently, deep learning (DL) methods, especially those based on convolutional neural network (CNN), have gained widespread application in plant disease classification. They have solved or partially solved the problems of traditional classification methods and represent state-of-the-art technology in this field. In this work, we reviewed the latest CNN networks pertinent to plant leaf disease classification. We summarized DL principles involved in plant disease classification. Additionally, we summarized the main problems and corresponding solutions of CNN used for plant disease classification. Furthermore, we discussed the future development direction in plant disease classification. Full article

The antioxidant activity of honey depends on the botanical origin, which also determines their physicochemical properties. In this study, a multivariate analysis was used to confirm potential relationships between the antioxidant properties and colour parameters, as well as the content of seven elements in five types of artisanal honey (rapeseed, buckwheat, linden, black locust, and multifloral). The type of honey was found to significantly influence most of its physicochemical properties, colour parameters, and the content of potassium, manganese and copper. Antioxidant parameters were shown to be significantly positively correlated with redness and concentrations of copper and manganese, but negatively correlated with the hue angle and lightness. The principal component analysis confirmed that the darkest buckwheat honey had the highest antioxidant activity in combination with its specific colour parameters and content of antioxidant minerals (manganese, copper and zinc). The level of these parameters can be potentially used for the identification of buckwheat honey. Full article

Organic fertilization has been proposed as an alternative approach to supply nutrients for crops, in the frame of organic and sustainable agriculture, with the aim to decrease high inorganic fertilization rates, protect the environment and decrease production costs for farmers. Since different types of organic fertilizers, such as manures, olive mill wastewater (OMW), sewage sludge (SS), crushed pruning wastes, composts and cover crops, exist as soil amendments to improve soil fertility, enhance plant nutrition and sustain the productivity of tree crops, their role as biofertilizers has been fully analyzed under the most important published papers. In addition, the benefits and drawbacks of organic fertilization, in a comparative approach with inorganic fertilization, are presented and discussed. Within the most important advantages of organic fertilizers, the enhancement of beneficial soil microorganisms and the improvement in soil physical properties and fertility should be included, while their most important disadvantage is their inability to directly satisfy the prompt N nutritional needs of tree crops, due to slow N mineralization rates. Finally, some novel aspects on the interrelation among innovative organic fertilizers for tree crops, sustainable field management, crop productivity and fruit quality are also included in this review, under the light of the most important and recent research data existing in the literature, with the aim to provide recommendations and future directions for organic fertilizers by tree growers. Full article

The use of urease inhibitors in irrigated systems decreases both soil ammonium (NH₄⁺) and nitrate (NO₃⁻) availability, and, thus, could be an easy tool to reduce N loss due to ammonia volatilization and NO₃⁻ leaching. The main goal of this experiment was to assess the effect of urease inhibitors on N use efficiency, N losses, and their economic impact in a maize-wheat field experiment. In this study, 10 treatments were compared, combining the urea fertilizer with or without urease inhibitor, applied in one or two dressings, and under optimal or sub-optimal irrigation. A single application of urease inhibitor (IN_1d), coupled with the conventional urea, helped to reduce the nitrate leaching risk both during the maize period (even when compared to the two dressing treatment) and after harvest. In addition, this improvement was achieved together with an increase in economic benefit, even when compared with the application of the same amount of regular urea split into two dressings. Under low water availability systems, the benefits of applying urease inhibitors increased with respect to the application of regular urea, making this technique a very promising strategy for adaptation to climate change in arid and semiarid regions. Full article

Nondestructive detection of tea’s internal quality is of great significance for the processing and storage of tea. In this study, hyperspectral imaging technology is adopted to quantitatively detect the content of tea polyphenols in Tibetan teas by analyzing the features of the tea spectrum in the wavelength ranging from 420 to 1010 nm. The samples are divided with joint x-y distances (SPXY) and Kennard-Stone (KS) algorithms, while six algorithms are used to preprocess the spectral data. Six other algorithms, Random Forest (RF), Gradient Boosting (GB), Adaptive boost (AdaBoost), Categorical Boosting (CatBoost), LightGBM, and XGBoost, are used to carry out feature extractions. Then based on a stacking combination strategy, a new two-layer combination prediction model is constructed, which is used to compare with the four individual regressor prediction models: RF Regressor (RFR), CatBoost Regressor (CatBoostR), LightGBM Regressor (LightGBMR) and XGBoost Regressor (XGBoostR). The experimental results show that the newly-built Stacking model predicts more accurately than the individual regressor prediction models. The coefficients of determination $R_c^2$ and$R_p^2$ for the prediction of Tibetan tea polyphenols are 0.9709 and 0.9625, and the root mean square error RMSEC and RMSEP are 0.2766 and 0.3852 for the new model, respectively, which shows that the content of Tibetan tea polyphenols can be determined with precision. Full article

Packing density has a significant influence on the outcome of ensiling forage. In this study, we aim to investigate the effect of packing density on the ensiling properties, microbiome, and in vitro digestibility of barley silages. Barley was ensiled in polyethylene drum silos (30 L) with respective densities of 600, 650, 700, and 750 kg/m³ fresh matter (FM), and stored for 60 days. The bacterial communities, fermentation quality, and in vitro digestibility were analyzed. Fresh barley had a low count of lactic acid bacteria (LAB, 10⁴ cfu/g of FM), and Lactobacillus was nearly undetectable (<1%). Increasing the packing density decreased the pH and the content of ammonia nitrogen (NH₃-N), ethanol, neutral detergent fiber (NDF), and acid detergent fiber (ADF) of barley silage (p < 0.05), and increased in vitro digestibility of dry matter, NDF, ADF, and DM recovery (p < 0.05). A higher packing density decreased the abundances of Enterobacter (from 47.4% to 35.4%) and Clostridium (from 13.5% to 3.8%), and increased the abundance of Lactobacillus (from 1.8% to 17.0%). Thus, packing density positively correlated with Lactobacillus (p < 0.05) but negatively correlated with Enterobacter (p < 0.05). The pH and the content of ethanol were positively correlated with Enterobacter (p < 0.05) but negatively correlated with Lactobacillus (p < 0.05). In conclusion, the density of 750 kg/m³ FM resulted in the highest silage quality of the densities tested. Full article

Progressive soil compaction is a disadvantage of intensive tillage. Compaction exerts a negative impact on the physical properties of soil and decreases crop performance. The adverse effects of soil compaction can be mitigated by replacing conventional tillage with simplified tillage techniques. Simplified tillage exerts a protective effect on soil, reduces production costs and preserves agricultural ecosystems. The aim of this study was to determine the influence of compaction and different tillage methods on the bulk density and moisture content of soil. The experimental factors were as follows: Soil compaction before sowing (non-compacted control treatment and experimental treatments where soil was compacted after the harvest of the preceding crop) and four different methods of seedbed preparation in a three-field rotation system (winter oilseed rape, winter wheat, spring barley). The influence of compaction on the bulk density and moisture content of soil varied across the rotated crops and their developmental stages. Soil compaction had no significant effect on the analyzed parameters in the cultivation of winter oilseed rape. In treatments sown with winter wheat, soil compaction resulted in significantly lower soil density and significantly higher soil moisture content. In plots sown with spring barley, soil compaction led to a significant increase in the values of both parameters. The average bulk density of soil after various tillage operations in the examined crop rotation system ranged from 1.49–1.69 g·m⁻³ (winter oilseed rape), 1.47–1.59 g·m⁻³ (winter wheat), 1.47–1.61 g·m⁻³ (spring barley). The bulk density and moisture content of soil were lowest after conventional tillage (control treatment) and higher after simplified tillage. Regardless of soil compaction, the greatest reduction in winter oilseed rape yields was noted in response to skimming, harrowing and the absence of pre-sowing plowing. Spring barley yields were higher in non-compacted treatments, whereas the reverse was observed in winter wheat. Chisel plowing and single plowing induced the greatest decrease in wheat yields relative to conventional tillage. Single plowing significantly decreased the grain yield of spring barley relative to the tillage system that involved skimming and fall plowing to a depth of 25. Full article

This study compares the individual phenolic response of husk tissues of Juglans regia L., infected to different degrees of severity with walnut anthracnose, which is one of the most serious and widespread walnut diseases worldwide. A comparison among three differently susceptible cultivars, ‘Franquette’, ‘Milotai 10’ (‘M10’), and ‘Milotai intenziv’ (‘M10-37’), is made. In our methodology, high performance liquid chromatography coupled with mass spectrometry is used to identify and quantify the compounds. Our results show that flavanols, flavonols, and naphthoquinones account for more than 95% of the phenolic compounds identified in the walnut husk. The higher total analyzed phenolic content in tissues is more affected by walnut anthracnose confirmed that phenolics play a major role in the plant’s response against pathogens. A difference between cultivars is observed, since French cultivar ‘Franquette’ responds differently to walnut anthracnose infection than Hungarian cultivars ‘M10’ and ‘M10-37’. Naphthoquinones and flavanols have a very similar response to walnut anthracnose infection. The resistance of cultivars may be due to the reaction time of the plant and the speed with which it recognizes the pathogen and responds quickly to the infection by containing it while it has not yet spread. Flavonols may be the most important phenolic compounds in disease control, since they respond more rapidly to infection than flavanols and naphthoquinones. They also play an inhibitory role in the early stages of viral and bacterial infections. Full article

Crop disease diagnosis is of great significance to crop yield and agricultural production. Deep learning methods have become the main research direction to solve the diagnosis of crop diseases. This paper proposed a deep convolutional neural network that integrates an attention mechanism, which can better adapt to the diagnosis of a variety of tomato leaf diseases. The network structure mainly includes residual blocks and attention extraction modules. The model can accurately extract complex features of various diseases. Extensive comparative experiment results show that the proposed model achieves the average identification accuracy of 96.81% on the tomato leaf diseases dataset. It proves that the model has significant advantages in terms of network complexity and real-time performance compared with other models. Moreover, through the model comparison experiment on the grape leaf diseases public dataset, the proposed model also achieves better results, and the average identification accuracy of 99.24%. It is certified that add the attention module can more accurately extract the complex features of a variety of diseases and has fewer parameters. The proposed model provides a high-performance solution for crop diagnosis under the real agricultural environment. Full article

As the market demand for button mushrooms is constantly growing, it is important to extend their shelf-life. Active packaging with built-in active components offers the possibility of extending the shelf life of products which are sensitive to external factors. Therefore, the aim of the present study was to check the effect of active packaging with zeolite (clinoptilolite) and aҫai extract on the bioactive compounds content, antioxidant activity, volatile compound profile, and physical quality of mushrooms subjected to storage for 28 days at 4 °C. Packing mushrooms in active packages improved their chemical characteristics by increasing antioxidant activity (p ≤ 0.001) in comparison to the conventional packaging methods. Moreover, it slowed down water loss and the browning process both on the surface and inside the mushroom. Furthermore, the results showed a strong correlation (p ≤ 0.001) between antioxidant activity, bioactive compounds content and color parameters. The obtained results suggest that the addition of the aҫai extract and zeolite into packaging material protects mushrooms from deterioration for a longer period of time. The use of active packaging to extend product shelf life can contribute to the reduction of the use of food preservatives, but also protects the environment by reducing the volume of waste. Full article

In order to create an irrigation scheduling plan for use in large-area citrus orchards, an environmental information collection system of citrus orchards was established based on the Internet of Things (IoT). With the environmental information data, deep bidirectional long short-term memory (Bid-LSTM) networks are proposed to improve soil moisture (SM) and soil electrical conductivity (SEC) predictions, providing a meaningful reference for the irrigation and fertilization of citrus orchards. The IoT system contains SM, SEC, air temperature and humidity, wind speed, and precipitation sensors, while the mean absolute error (MAE), root mean square error (RMSE), and coefficient of determination (R²) were calculated to evaluate the performance of the models. The performance of the deep Bid-LSTM model was compared with a multi-layer neural network (MLNN). The results for the performance criteria reveal that the proposed deep Bid-LSTM networks perform better than the MLNN model, according to many of the evaluation indicators of this study. Full article

Pitaya (Hylocereus polyrhizus L.) is highly tolerant to drought stress. Elucidating the response mechanism of pitaya to drought will substantially contribute to improving crop drought tolerance. In the present study, the physiological and proteomic responses of the pitaya cultivar ‘Zihonglong’ were compared between control seedlings and seedlings exposed to drought stress (−4.9 MPa) induced by polyethylene glycol for 7 days. Drought stress obviously enhanced osmolyte accumulation, lipid peroxidation, and antioxidant enzyme activities. Proteomic data revealed drought stress activated several pathways in pitaya, including carbohydrate and energy metabolism at two drought stress treatment time-points (6 h and 3 days). Other metabolic pathways, including those related to aspartate, glutamate, glutathione, and secondary metabolites, were induced more at 3 days than at 6 h, whereas photosynthesis and arginine metabolism were induced exclusively at 6 h. Overall, protein expression changes were consistent with the physiological responses, although there were some differences in the timing. The increases in soluble sugar contents mainly resulted from the degradation and transformation of insoluble carbohydrates. Differentially accumulated proteins in amino acid metabolism may be important for the conversion and accumulation of amino acids. GSH and AsA metabolism and secondary metabolism may play important roles in pitaya as enzymatic and nonenzymatic antioxidant systems. The enhanced carbohydrate and energy metabolism may provide the energy necessary for initiating the above metabolic pathways. The current study provided the first proteome profile of this species exposed to drought stress, and may clarify the mechanisms underlying the considerable tolerance of pitaya to drought stress. Full article

Sulphur plays crucial roles in plant growth and development, with its functions ranging from being a structural constituent of macro-biomolecules to modulating several physiological processes and tolerance to abiotic stresses. In spite of these numerous sulphur roles being well acknowledged, agriculture has paid scant regard for sulphur nutrition, until only recently. Serious problems related to soil sulphur deficiencies have emerged and the intensification of food, fiber, and animal production is escalating to feed the ever-increasing human population. In the wake of huge demand for high quality cereal and vegetable diets, sulphur can play a key role in augmenting the production, productivity, and quality of crops. Additionally, in light of the emerging problems of soil fertility exhaustion and climate change-exacerbated environmental stresses, sulphur assumes special importance in crop production, particularly under intensively cropped areas. Here, citing several relevant examples, we highlight, in addition to its plant biological and metabolism functions, how sulphur can significantly enhance crop productivity and quality, as well as acclimation to abiotic stresses. By this appraisal, we also aim to stimulate readers interests in crop sulphur research by providing priorities for future pursuance, including bettering our understanding of the molecular processes and dynamics of sulphur availability and utilization in plants, dissecting the role of soil rhizospherical microbes in plant sulphur transformations, enhancing plant phenotyping and diagnosis for nutrient deficiencies, and matching site-specific crop sulphur demands with fertilizer amendments in order to reduce nutrient use inefficiencies in both crop and livestock production systems. This will facilitate the proper utilization of sulphur in crop production and eventually enhance sustainable and environmentally friend food production. Full article

Green roofs are an important part of urban green spaces. A good roof soil system contributes to the stability of the green roof ecosystem in harsh environments. Biochar as a soil additive can improve soil nutrients, although the mechanism of improvement on the roof substrate is still unclear. This research studied the effects on the physical and chemical properties of green roof soil and analyzed the biological characteristics of green roofs at five gradient ratios of biochar addition (0%, 5%, 10%, 15% and 20% biochar; v/v). The results indicated that biochar could improve the soil porosity (5.3–9.3%) and reduce the bulk density (3.9–10.8%); increase the soil moisture (14.0–37.2%); adjust the temperature, available nutrients and cation exchange capacity (38.1–75.9%) and regulate the soil pH values of the green roof. The biomass of soil microbes, eukaryotes and plants were increased by 75.3%, 199.2% and 57.5%, respectively. Meanwhile, the correlation between microbial diversity and soil nutrients was more significant due to the addition of biochar, and the increase of the phosphorus (P) and carbon (C) contents was the main factor affecting the microbial community. The structural equation model showed that biochar has a direct impact on the microbial diversity by improving the soil moisture, temperature and available nutrients, and the increase of the microbial diversity is conducive to plant growth. Summarily, biochar can be considered as a potential additive for roof soil amendment and promoting the growth of plants and microbes, which is beneficial to the development of a roof ecosystem. Full article

Spatial and temporal variability characterization in Precision Agriculture (PA) practices is often accomplished by proximity data gathering devices, which acquire data from a wide variety of sensors installed within the vicinity of crops. Proximity data acquisition usually depends on a hardware solution to which some sensors can be coupled, managed by a software that may (or may not) store, process and send acquired data to a back-end using some communication protocol. The sheer number of both proprietary and open hardware solutions, together with the diversity and characteristics of available sensors, is enough to deem the task of designing a data acquisition device complex. Factoring in the harsh operational context, the multiple DIY solutions presented by an active online community, available in-field power approaches and the different communication protocols, each proximity monitoring solution can be regarded as singular. Data acquisition devices should be increasingly flexible, not only by supporting a large number of heterogeneous sensors, but also by being able to resort to different communication protocols, depending on both the operational and functional contexts in which they are deployed. Furthermore, these small and unattended devices need to be sufficiently robust and cost-effective to allow greater in-field measurement granularity 365 days/year. This paper presents a low-cost, flexible and robust data acquisition device that can be deployed in different operational contexts, as it also supports three different communication technologies: IEEE 802.15.4/ZigBee, LoRa/LoRaWAN and GRPS. Software and hardware features, suitable for using heat pulse methods to measure sap flow, leaf wetness sensors and others are embedded. Its power consumption is of only 83 $\mathsf{\mu }$A during sleep mode and the cost of the basic unit was kept below the EUR 100 limit. In-field continuous evaluation over the past three years prove that the proposed solution—SPWAS’21—is not only reliable but also represents a robust and low-cost data acquisition device capable of gathering different parameters of interest in PA practices. Full article

The automatic detection of diseases in plants is necessary, as it reduces the tedious work of monitoring large farms and it will detect the disease at an early stage of its occurrence to minimize further degradation of plants. Besides the decline of plant health, a country’s economy is highly affected by this scenario due to lower production. The current approach to identify diseases by an expert is slow and non-optimal for large farms. Our proposed model is an ensemble of pre-trained DenseNet121, EfficientNetB7, and EfficientNet NoisyStudent, which aims to classify leaves of apple trees into one of the following categories: healthy, apple scab, apple cedar rust, and multiple diseases, using its images. Various Image Augmentation techniques are included in this research to increase the dataset size, and subsequentially, the model’s accuracy increases. Our proposed model achieves an accuracy of 96.25% on the validation dataset. The proposed model can identify leaves with multiple diseases with 90% accuracy. Our proposed model achieved a good performance on different metrics and can be deployed in the agricultural domain to identify plant health accurately and timely. Full article

In order to determine the effects of nitrogen fertilizer on the grain quality and starch physicochemical properties of japonica rice cultivars with different contents of amylose, normal amylose content (NAC) and low amylose content (LAC) cultivars were grown in a field, with or without nitrogen fertilizer (WN). The relationships between the amylose content, starch physicochemical properties and eating quality were also examined. Compared with WN, nitrogen fertilizer (NF) significantly increased the grain yield but markedly decreased the grain weight. In addition, the processing quality tended to improve, but the appearance quality and eating quality deteriorated under NF application. The grain yield was similar between NAC and LAC cultivars. However, the grain quality and starch physicochemical properties were significantly different between NAC and LAC cultivars. The palatability of the cooked rice was significantly higher in the LAC than in NAC cultivar, which was due to its lower amylose content, protein content, hardness, and retrogradation enthalpy and degree, and its higher stickiness, peak viscosity, breakdown, relative crystallinity and peak intensity. The amylose content and protein content were significantly negatively correlated with the palatability. The amylose content was significantly positively correlated with the final viscosity and setback, and was significantly negatively correlated with the relative crystallinity, peak intensity, gelatinization enthalpy and breakdown. Palatability was significantly positively correlated with peak viscosity, breakdown and peak intensity, and was significantly negatively correlated with the final viscosity, setback, and retrogradation enthalpy and degree. Therefore, the selection of a low amylose content japonica rice cultivar grown without nitrogen fertilizer can reduce the amylose and protein contents, as well as improving the pasting properties, starch retrogradation properties and eating quality of the cooked rice. Full article

In response to urgent demand to raise awareness of the nutritional and health benefits of tomato consumption and to advocate for healthy diets through increased sustainable production and consumption of fruits and vegetables, this study is intended to promote a healthy and balanced lifestyle, sharing the best practices of production and consumption. The aim of this research was to compare the effects of the growing system (field vs. plastic tunnel) and of genotype characteristics for organic improved tomato genotypes. The research was carried out in the 2019 and 2020 years on eight improved tomato genotypes. The results showed that the ascorbic acid content presented higher values for organic tomatoes cultivated in the field for all genotypes studied, with an upper limit of 18.57 mg·100 g⁻¹ FW. In contrast, the content in β-carotene and lycopene showed higher values for genotypes grown under plastic tunnel conditions. Significant statistical differences were noticed concerning the mean values of all genotypes according to cultivation conditions (field vs. plastic tunnel) for most parameters excepting total soluble solids (TSS), titratable acidity (TA), maturity index (MI) and flavor index (FI). This highlights the major importance of the selection of some genotypes of tomatoes that respond positively to the organic cultivation system in terms of the presence of the antioxidants compounds (vitamin C, lycopene, and carotene) in representative quantities. Genotype 3 is highlighted by the highest content in carotene (7.4 mg·100 g⁻¹ F.W.) and lycopene (8.4 mg·100 g⁻¹ F.W.) and genotype 5 by the highest content in vitamin C (16.8 mg·100 g⁻¹ F.W.). The results of the study suggest that by applying appropriate techniques for growing organic tomatoes in the plastic tunnel system, the antioxidant substrate can be optimized compared to the results obtained for the field system. Full article

Rice (Oryza sativa L.) is one of the most important cereal crops in the world. The identification of sd1 mutants in rice resulted in a semi-dwarf phenotype that was used by breeders to improve yields. Investigations of sd1 mutants initiated the “green revolution” for rice and staved off famine for many people in the 1960s. The smaller plant height conferred by sd1 allele gives the plants lodging resistance even with a high amount of nitrogen fertilizer. Guang-chang-ai-carrying sd1 was the first high-yielding rice variety that capitalized on the semi-dwarf trait, aiming to significantly improve the rice yield in China. IR8, known as the miracle rice, was also bred by using sd1. The green revolution gene sd1 in rice has been used for decades, but was not identified for a long time. The SD1 gene encodes the rice Gibberellin 20 oxidase-2 (GA20ox2). As such, the SD1 gene is instrumental in uncovering the molecular mechanisms underlying gibberellin biosynthesis There are ten different alleles of SD1. These alleles are identified by genome sequencing within several donor lines in breeding for semi-dwarf rice. Apart from breeding applications and the molecular mechanism of GA biosynthesis, the SD1 gene is also involved in the molecular regulation of other important agronomic traits, like nitrogen fertilizer utilization. The dentification of new alleles of SD1 can be obtained by mutagenesis and genome editing. These new alleles will play an important role in improving the resource diversity of semi-dwarf breeding in the future. Full article

The European legislative on the use of different control strategies against plant-parasitic nematodes, with particular reference to pesticides, is constantly evolving, sometimes causing confusion in the sector operators. This article highlights the nematode control management allowed in the C Zone of the European Union, which includes the use of chemical nematicides (both fumigant and non-fumigant), agronomic control strategies (crop rotations, biofumigation, cover crops, soil amendments), the physical method of soil solarization, the application of biopesticides (fungi, bacteria and their derivatives) and plant-derived formulations. The authors analyze the use of these strategies and substances in organic agriculture as well as in Integrated Pest Management (IPM) programs. Full article

The development of precision grass measurement technologies is of vital importance to securing the future sustainability of pasture-based livestock production systems. There is potential to increase grassland production in a sustainable manner by achieving a more precise measurement of pasture quantity and quality. This review presents an overview of the most recent seminal research pertaining to the development of precision grass measurement technologies. One of the main obstacles to precision grass measurement, sward heterogeneity, is discussed along with optimal sampling techniques to address this issue. The limitations of conventional grass measurement techniques are outlined and alternative new terrestrial, proximal, and remote sensing technologies are presented. The possibilities of automating grass measurement and reducing labour costs are hypothesised and the development of holistic online grassland management systems that may facilitate these goals are further outlined. Full article

Probiotics are live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. Traditionally, dairy products are the major and most popular probiotic carriers. At present, there is a growing demand for non-dairy probiotic products. Both fermented and non-fermented non-dairy plant-based food products are becoming highly appealing to both dairy and non-dairy consumers worldwide. Non-dairy plant-based food matrices such as fruits, vegetables, plant-based milk, cereals, and legumes have been used successfully in producing probiotic products with the minimum recommended viable probiotic numbers at the time of consumption. However, due to the exclusion of dairy, whether these food matrices can enhance the functional properties of probiotics such as gastrointestinal survival and immune-enhancing effects needs a thorough investigation. Hence, this review focuses on some of the popular non-dairy plant-based probiotic food products and their microbiological quality characteristics in terms of maintaining probiotic viability during product storage. Their gastrointestinal tolerance in these products, other functional properties, and product qualities have also been briefly discussed. Full article

Forests across the world are considered to be a huge socio-economic and environmental benefit to host and adjacent communities. This study focuses on assessing the impacts of fuelwood and timber consumption on the livelihood of households in the Baltistan region in Pakistan. Primary and secondary sources of data were employed for the study. The primary sources involved the use of questionnaire survey and interview while the secondary sources involved the use of documented information in textbooks and internet materials. The study revealed that 82% of the people within the region were involved in agricultural activities, 71% depended on the extraction of forest resources for their livelihood, while 18% depended on off-farm activities for their livelihood. The study also observed that among the number that depended on forest resources for their livelihood, 59% were involved in the extraction of non-timber forest products while 41% were involved in the extraction of timber forest resources. The study further revealed that there was no significant difference in the level of benefits from the forest across the seven districts under investigation with a chi square value. The volume of forest products extraction was found to be high closest to the forest and to be low with increasing distance from the communities. The major benefits from the forest range were due to employment that increases the individual and family income. Forest also helps to control erosion and enhances aesthetic beautification and temperature regulation. The research suggests that the policy makers must provide a sustainable solution to reduce the overexploitation of the forest resources by providing better alternative earning resources to the resident communities. Full article

Investigating the interactions of granular fertilizers with various types of equipment is an essential part of agricultural research. A numerical technique simulating the mechanical behavior of granular assemblies has the advantage of data trackings, such as the trajectories, velocities, and transient forces of the particles at any stage of the test. The interaction parameters were calibrated to simulate responses of granular fertilizers in EDEM, a discrete element method (DEM) software. Without a proper calibration of the interaction parameters between the granular fertilizers and various materials, the simulations may not represent the real behavior of the granular fertilizers. Therefore, in this study, a strategy is presented to identify and select a set of DEM input parameters of granular fertilizers using the central composite design (CCD) to establish the nonlinear relationship between the dynamic macroscopic granular fertilizer properties and the DEM parameters. The determined interaction properties can be used to simulate granular fertilizers in EDEM. Full article

Ecosystem-based approaches to nutrient management are needed to satisfy crop nutrient requirements while minimizing environmental impacts of fertilizer use. Applying crop residues as soil amendments can provide essential crop nutrient inputs from organic sources while improving nutrient retention, soil health, water conservation, and crop performance. Tree crop hulls, husks, and shells have been found to contain high concentrations of potassium across species including almond, cacao, coffee, pecan, and hazelnut. The objective of this review is to characterize organic sources of potassium focusing on lignocellulosic pericarps and discuss reported effects of surface application on potassium cycling, water dynamics, soil functionality, and crop yield. Research indicates potassium ions solubilize readily from plant material into soil solution due to potassium’s high mobility as a predominately unbound monatomic cation in plant tissues. Studies evaluating tree crop nutshells, field crop residues, and forest ecosystem litter layers indicate this process of potassium release is driven primarily by water and is not strongly limited by decomposition. Research suggests orchard floor management practices can be tailored to maximize the soil and plant benefits provided by this practice. Contextual factors influencing practice adoption and areas for future study are discussed. Full article

A scientific justification, focused on the development of the sustainability of feed ingredients and farm animals’ ecosystems, is required. Thus, it is crucial to search for alternative feed materials from natural sources for potential applications. The aim of this study was to evaluate the prospective utilization of freshwater Cladophora glomerata (C. glomerata) as an alternative source of protein and other essential nutrients in animals’ feed. For this purpose, chemical analysis was performed on collected biomass samples from the Lithuanian rivers, Dubysa (B1), Šventoji (B2), Nevėžis (B3), and Jūra (B4). Microelements (Ca > K > N > P > Mg), trace elements (Zn > Cu), and heavy metals (Cr > Ni > Pb > Cd) have not exceeded permissible levels. The crude protein content of C. glomerata biomass ranged from 16% to 21.5% DM. The essential amino acid profile excelled, with the highest total amino acid amount of 140.99 g/kg in B4. The highest total presence of polyunsaturated fatty acids (11.71%) as well as the ratio of polyunsaturated to saturated fatty acids (0.22) was observed in B1. The lowest ratio of omega-6/omega-3 was in B1 (1.30). As a result of bioaccumulation, C. glomerata could serve as a source of proteins, as well as amino and fatty acids, implying that biomass could be an alternative and a beneficial component of animal feed. Full article

Given that farmland serves as a strategic resource to ensure national food security, blind emphasis on the improvement of food production capacity can lead to soil overutilization and impair other soil functions. Hence, the evaluation of soil health (SH) should comprehensively take soil productivity and ecological environmental effects into account. In this study, five functions from the perspective of functional soil management were summarized, including primary productivity, provision and cycling of nutrients, the provision of functional and intrinsic biodiversity, water purification and regulation, and carbon sequestration and regulation. For each soil function, in view of the natural and ameliorable conditions affecting SH, basic indicators were selected from the two aspects of inherent and dynamic properties, and restrictive indicators were chosen considering the external properties or environmental elements, with the minimum limiting factor method coupled with weighted linear model. The new evaluation system was tested and verified in Yixing City, China. The healthy and optimally functional soils were concentrated in the northeast and mid-west of Yixing City, whereas unhealthy soils were predominant in the south and around Taihu Lake. The main limitations to SH improvement included cation exchange capacity, nutrient elements, and soluble carbon. The SH evaluation method was verified using the crop performance validation method, and a positive correlation was noted between food production stability index and soil health index, indicating that the evaluation system is reasonable. Full article

Among the waste by-products generated by the fruit industry (peels, seeds, and skins), fruit peel constitutes the major component. It is estimated that fruit peel accounts for at least 20% of the fresh fruit weight. Fruit peels are considered as major sources of dietary fiber and anticipated to be successfully utilized as prebiotics. This study examined the chemical composition, functional properties and the prebiotic effects of three major tropical fruit peels (apple, banana and mango). The prebiotic effect was tested using three commercial probiotic strains (Lactobacillus rhamnosus, L. casei and Bifidobacterium lactis) individually and in combination. Each probiotic culture was fortified with different concentration (0%, 2% and 4%) of selected fruit peel powder (FPP). Results revealed that all tested FPP significantly (p < 0.05) enhanced the probiotics viable counts, which reached >10 logs after 24 h of incubation. However, the concentration of 2% and 4% FPP showed no significant differences (p > 0.05) on the probiotic viable counts. Additionally, the prebiotic effects of FPP were the same when applied to individual and mixed cultures. This investigation demonstrated that small amount (2%) of apple, banana and mango peel powder could be successfully utilized as prebiotics to enhance the growth of lactic acid bacteria (LAB). Additionally, the studied physical and chemical characteristics of FPP demonstrated their potential applications in the food and pharmaceutical industries as functional ingredients. Full article

The objective of the present study was to determine the content and bioaccessibility of carotenoids in 104 maize hybrids available at the market. Lutein, zeaxanthin, α- and β-cryptoxanthin, and β-carotene were determined in whole grains and micelles produced during standardized INFOGEST digestibility analysis, and their bioaccessibility was calculated as the ratio of micellar and grain carotenoids. Tested hybrids varied in total carotenoid content, with 34% having total carotenoid content in the range of 15–20 µg/g dry matter (DM) and 41% in the range of 20–25 µg/g DM. The amount of bioaccessible carotenoids increased linearly (p < 0.05) with increasing content in the grain, and decreased among determined carotenoidsd in the order: lutein (52%) > zeaxanthin (43%) > β-carotene (43%) > α-cryptoxanthin (27%) > β-cryptoxanthin (26%). Bioaccessibility of lutein, zeaxanthin, and β-carotene decreased with increasing content in the grain (p < 0.05). On average, only 43% of the total carotenoids were bioaccessible in commercial maize hybrids tested, which should be considered when formulating an animal diet. Full article

Subsoiling has been acknowledged worldwide to break compacted hardpan, improve soil permeability and water storage capacity, and promote topsoil deepening and root growth. However, there exist certain factors which limit the wide in-field application of subsoiling machines. Of these factors, the main two are poor subsoiling quality and high energy consumption, especially the undesired tillage depth obtained in the field with cover crops. Based on the analysis of global adoption and benefits of subsoiling technology, and application status of subsoiling machines, this article reviewed the research methods, technical characteristics, and developing trends in five key aspects, including subsoiling shovel design, anti-drag technologies, technologies of tillage depth detection and control, and research on soil mechanical interaction. Combined with the research progress and application requirements of subsoiling machines across the globe, current problems and technical difficulties were analyzed and summarized. Aiming to solve these problems, improve subsoiling quality, and reduce energy consumption, this article proposed future directions for the development of subsoiling machines, including optimizing the soil model in computer simulation, strengthening research on the subsoiling mechanism and comprehensive effect, developing new tillage depth monitoring and control systems, and improving wear-resisting properties of subsoiling shovels. Full article

The rapid development of cities in the recent 10 years caused a reduction in the cultivated land area, which only accounts for 14% of the total land area in China. Land development and reclamation have been regarded as an effective way to compensate farmland occupation. However, most of the newly reclaimed land has poor soil fertility and suitability; in some cases, the production capacity is only 10–30% of the occupied farmland. In order to ameliorate the soil quality of the newly reclaimed land, this study evaluated the effects of commercial organic fertilizer (0.75, 1.50, and 2.25 kg/m²), mushroom residue (1.50, 2.25, and 3.00 kg/m²), biogas slurry (150, 225, and 300 kg/m²), vegetable cake (0.30 and 0.60 kg/m²), and chemical compound fertilizer (37.50 g/m²) on the pH, moisture content and organic matter content (OMC), available phosphate, total nitrogen, alkaline hydrolysis nitrogen, microbial biomass carbon and nitrogen, and number of total bacteria and phosphate-solubilizing bacteria, as well as the growth of maize seedlings. The results from this study indicate that the soil quality (OMC is an indicator) was ameliorated by chemical and organic fertilizers, in particular commercial organic fertilizers, which caused a 9.35–16.35% increase in moisture content, a 11.56–18.72% increase in pH, a 1.73–2.15 fold increase in OMC, a 338.44–491.41% increase in available P, a 36.80–48.14% increase in total N, a 95.32–128.34% increase in alkaline hydrolysis N, a 92.57–178.38% increase in total bacterial numbers, and a 7.57–20.87 fold increase in microbial biomass carbon compared with the control. The pot experiment further indicated that soil amended with commercial organic fertilizers caused a 20.35–30.55% increase in the height and a 12.50–16.67% increase in the total dry weight of maize seedlings. In addition, representative strains with the ability to dissolve phosphorus and fix nitrogen were successfully isolated using the culture method, and were then identified based on colony morphological observation and 16S rDNA sequence analysis, which help us to not only understand why organic fertilizer has great effect on soil improvement, but also provides beneficial microbial resources for further study. Full article

Rice yield is a complex trait that is strongly affected by environment and genotype × environment interaction (GEI) effects. Consideration of GEI in diverse environments facilitates the accurate identification of optimal genotypes with high yield performance, which are adaptable to specific or diverse environments. In this study, multiple environment trials were conducted to evaluate grain yield (GY) and four yield-component traits: panicle length, panicle number, spikelet number per panicle, and thousand-grain weight. Eighty-nine rice varieties were cultivated in temperate, subtropical, and tropical regions for two years. The effects of both GEI (12.4–19.6%) and environment (23.6–69.6%) significantly contributed to the variation of all yield-component traits. In addition, 37.1% of GY variation was explained by GEI, indicating that GY performance was strongly affected by the different environmental conditions. GY performance and genotype stability were evaluated using simultaneous selection indexing, and 19 desirable genotypes were identified with high productivity and broad adaptability across temperate, subtropical, and tropical conditions. These optimal genotypes could be recommended for cultivation and as elite parents for rice breeding programs to improve yield potential and general adaptability to climates. Full article

Rice straw is a byproduct of agricultural production and an important agricultural resource. However, rice straw has not yet been effectively used, and incorrect treatment methods (such as burning in the field) can cause serious damage to the environment. Studies have shown that straw returning is beneficial to soil, but there have been few studies focused on the effect of the amount of short-term straw returned on the soil microbial community. This study evaluates 0%, 50%, 75%, and 100% rice straw returned to the field on whether returning different amounts of straw in the short term would affect the diversity and composition of the soil microbial community and the correlation between bacteria and fungi. The results show that the amount of straw returned to the field is the main factor that triggers the changes in the abundance and composition of the microbial community in the paddy soil. A small amount of added straw (≤50% straw added) mainly affects the composition of the bacterial community, while a larger amount of added straw (>50% straw added) mainly affects the composition of the fungal community. Returning a large amount of straw increases the microbial abundance related to carbon and iron cycles in the paddy soil, thus promoting the carbon and iron cycle processes to a certain extent. In addition, network analysis shows that returning a large amount of straw also increases the complexity of the microbial network, which may encourage more microbes to be niche-sharing and comprehensively improve the ecological environment of paddy soil. This study may provide some useful guidance for rice straw returning in northeast China. Full article

Water deficit is one of the most problematic stressors worldwide. In this context, the use of biostimulants represents an increasingly ecological practice aimed to improve crop tolerance and mitigate the negative effects on the productivity. Here, the effect derived from the foliar application of ERANTHIS^®®, a biostimulant based on seaweed (Ascophyllum nodosum and Laminaria digitata) and yeast extracts, was tested on tomato plants grown under mild water-stress conditions. The potential stress mitigation action was evaluated by monitoring morphometric (fresh weight and dry matter content), physiological (stem water potential) and biochemical (ROS scavenger enzymes activity, proline, abscisic acid, hydrogen peroxide and photosynthetic pigment content) parameters closely related to the occurrence and response to stress at both flowering and fruit-set timing. In general, we observed that plants grown under drought conditions and treated with the biostimulant had a lower amount of ABA, and MDA and proline correlated to a lower activity of ROS scavenger enzymes compared to untreated plants. These data, together with the higher stem water potential and photosynthetic pigment levels recorded for the treated plants, suggest that ERANTHIS^®® may mitigate water stress effects on tomato. Full article

The need for a transition to sustainable food systems is widely recognised. Over the last three decades, movements have been demanding and proposing a radical transformation, foregrounding the social values of food. Experiences inspired by solidarity economy have given rise to highly innovative pathways, grounded on the redefinition of the food-related values and practices and the reconstruction of local, community-based food systems by referring to social and ecological sustainability. One can usefully draw from these experiences for identifying challenges, opportunities and benefits and for analysing the most effective modes of action leading to the creation of alternatives. Capturing and supporting this innovation is particularly important when looking at the opportunities offered by local food policies. This significantly involves the meanings, goals and forms that food governance takes on. The paper aims at investigating these aspects, reading the initiatives inspired by SE principles as an example of social innovation. Their engagement in re-signifying food in terms of “commons” and in “commoning” food systems constitutes a complementary key of analysis. Focusing on the Italian context, the paper draws on many years of qualitative research and direct involvement in these initiatives. The analysis provides useful insights about the potential for change existing in society and invites us to develop reflexivity on how local food policies capture the opportunity for a re-politicisation of food-related issues. Full article

Late pear cultivars, such as ‘Conference’, can be stored for a long period if kept in good storage conditions. A three-year study (2011–2013) compared the impact of six-month storage using four technologies—normal atmosphere, normal atmosphere + 1-methylcyclopropene (1-MCP), controlled atmosphere, and controlled atmosphere + 1-MCP—on the quality parameters of ‘Conference’ pears, such as mass loss, firmness, total soluble solids, acidity, antioxidant capacity, and the incidence of diseases and disorders. Additionally, the study analysed different storage conditions in terms of profitability, based on the market prices for pears in the seasons during which the pears were stored. The storage conditions had a very strong influence on the fruit quality parameters, and were found to affect most visibly the mass loss and the incidence of postharvest diseases and disorders. The storage of ‘Conference’ pears for 180 days in normal atmosphere is not economically viable, even if the fruit is subjected to 1-MCP treatment; at the same time, it is profitable to store ‘Conference’ pears in controlled atmosphere for the same period, no matter whether 1-MCP was applied or not. Full article

Lipases are enzymes that catalyze various types of reactions and have versatile applications. Additionally, lipases are the most widely used class of enzymes in biotechnology and organic chemistry. Lipases can be produced by a wide range of organisms including animals, plants and microorganisms. Microbial lipases are more stable, they have substrate specificity and a lower production cost as compared to other sources of these enzymes. Although commercially available lipases are widely used as biocatalysts, there are still many challenges concerning the production of microbial lipases with the use of renewable sources as the main component of microbial growth medium such as straw, bran, oil cakes and industrial effluents. Submerged fermentation (SmF) and solid-state fermentation (SSF) are the two important technologies for the production of lipases by microorganisms. Therefore, this review focuses on microbial lipases, especially their function, specificity, types and technology production, including the use of renewable agro-industrial residues and waste materials. Full article

In light of foreseen global climatic changes, we can expect crops to be subjected to several stresses that may occur at the same time, but information concerning the effect of long-term exposure to a combination of stresses on fruit yield and quality is scarce. This work looks at the effect of a long-term combination of salinity and high temperature stresses on tomato yield and fruit quality. Salinity decreased yield but had positive effects on fruit quality, increasing TSS, acidity, glucose, fructose and flavonols. High temperatures increased the vitamin C content but significantly decreased the concentration of some phenolic compounds (hydroxycinnamic acids and flavanones) and some carotenoids (phytoene, phytofluene and violaxanthin). An idiosyncrasy was observed in the effect of a combination of stresses on the content of homovanillic acid O-hexoside, lycopene and lutein, being different than the effect of salinity or high temperature when applied separately. The effect of a combination of stresses may differ from the effects of a single stress, underlining the importance of studying how stress interactions may affect the yield and quality of crops. The results show the viability of exploiting abiotic stresses and their combination to obtain tomatoes with increased levels of health-promoting compounds. Full article

In the past decade Colletotrichum gloeosporioides, C. karstii, and Alternaria alternata represent emerging fungal pathogens on citrus in the Mediterranean basin. Selection of tolerant Citrus germplasm offers evaluable long-term solution and should be considered as promising alternative to limit synthetic fungicide application to manage Alternaria and Colletotrichum infections in Citrus groves. In this study, the high variability of pre-harvest disease symptoms among 37 Citrus accessions was investigated in Italy in the most representative Sicilian production districts covering 1500 ha and including 20 homogeneous areas over an eleven-year survey period (2010–2020). Early fruit drop, brown spot, and anthracnose on fruit and leaves, pre-harvest fruit drop, and twig blight and defoliation associated to Alternaria and Colletotrichum spp. were identified on oranges, mandarins, and lemons. Comprehensively, first extensive data obtained herein on field susceptibility within “Tarocco” blood orange group to above disease symptoms clearly indicate as “Tarocco Nucellare 57-1E-1,” “Tarocco Tapi,” “Tarocco Sant’Alfio,” and “Tarocco Catania” accessions should be preferred to remaining Tarocco ones. A broad degree of tolerance or susceptibility was also observed within other Citrus group as it happens for tolerant lemon “Femminello Zagara Bianca,” thus demonstrating a putative resource for further studies to employ in a breeding program for genetic improvement of Citrus. Full article