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From the first issue of 2016, Chromatography has changed its name to Separations.

Open AccessArticle
Chromatography 2015, 2(3), 410-421; doi:10.3390/chromatography2030410

Determination of Sesquiterpenes in Wines by HS-SPME Coupled with GC-MS

Department of Chemical Science, University of Messina, Viale F. Stagno d'Alcontres, 31 98168 Messina, Italy
Author to whom correspondence should be addressed.
Academic Editor: Mary Boyce
Received: 26 March 2015 / Revised: 24 June 2015 / Accepted: 3 July 2015 / Published: 9 July 2015
(This article belongs to the Special Issue Solid Phase Micro-Extraction)
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The sesquiterpene compounds present in red wines were characterized and quantified by Headspace Solid-Phase Microextraction in combination with Gas Chromatography–Mass Spectrometry (HS-SPME-GC-MS). Sixteen sesquiterpenes were identified, mainly hydrocarbons but also derived oxygenated compounds. Sesquiterpenes were acyclic, monocyclic, byciclic and tryciclic. Sesquiterpenes were detected in SIM (selected ion monitoring) mode using their characteristics ions. All the sesquiterpenes were identified by mass spectral data, linear retention indices (LRI), literature data and injection of standards where available. Quantitative results were obtained using the method of standard additions. The method showed an average LOD = 0.05 µg L−1 and LOQ = 0.15 µg L−1. The monocyclic sesquiterpene with the germacrene skeleton, Germacrene D and the bicyclic sesquiterpene with the muurolane skeleton, α-muurolene were present in all the wine samples analysed. Syrah wines were the samples richest in sesquiterpenes in agreement with their typical spicy and woody notes. The results evidenced the possibility to use sesquiterpenes for wine authenticity and traceability. View Full-Text
Keywords: sesquiterpenes; red wines; HS-SPME-GC-MS sesquiterpenes; red wines; HS-SPME-GC-MS

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Cincotta, F.; Verzera, A.; Tripodi, G.; Condurso, C. Determination of Sesquiterpenes in Wines by HS-SPME Coupled with GC-MS. Chromatography 2015, 2, 410-421.

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