Effects of Different Levels of Echinostoma caproni Miracidial Dose on Glucose and Maltose Composition of Biomphalaria glabrata Snails as Determined by High Performance Thin-Layer Chromatography-Densitometry
Abstract
:1. Introduction
2. Materials and Methods
2.1. Snail Collection and Maintenance
2.2. Experimental Design
2.3. Snail Exposure
2.4. Sample Collection and Preparation
2.5. Standard Solution Preparation
2.6. HPTLC Analysis
2.7. Statistical Significance
3. Results and Discussion
3.1. Young Adult Snails
Maltose a | Glucose | |||
---|---|---|---|---|
Group | 2 Week Necropsy | 4 Week Necropsy | 2 Week Necropsy | 4 Week Necropsy |
A | 0.250 ± 0.13 | 0.318 ± 0.20 | 0.429 ± 0.30 | 0.485 ± 0.11 |
B | 0.332 ± 0.065 b | 0.603 ± 0.10 | 0.384 ± 0.10 | 0.708 ± 0.26 |
C | 0.105 ± 0.047 | 0.380 ± 0.20 | 0.475 ± 0.24 | 0.428 ± 0.074 |
D | 0.142 ± 0.039 | 0.136 ± 0.061 c | 0.151 ± 0.024 d | 0.178 ± 0.066 e |
3.2. Mature Adult Snails
Maltose a b | Glucose c | |||
---|---|---|---|---|
Group | 2 Week Necropsy | 4 Week Necropsy | 2 Week Necropsy | 4 Week Necropsy |
A | 0.364 ± 0.068 | 0.585 ± 0.092 | 0.465 ± 0.20 | 0.643 ± 0.035 |
B | 0.324 ± 0.065 | 0.448 ± 0.13 | 0.560 ± 0.020 | 0.584 ± 0.048 |
C | 0.325 ± 0.052 | 0.483 ± 0.11 | 0.616 ± 0.17 | 0.470 ± 0.16 |
D | 0.0835 ± 0.017 | 0.140 ± 0.022 | 0.106 ± 0.044 | 0.110 ± 0.040 |
3.3. Discussion
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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DeGrandchamp, D.; Hartlaub, S.; Fried, B.; Sherma, J. Effects of Different Levels of Echinostoma caproni Miracidial Dose on Glucose and Maltose Composition of Biomphalaria glabrata Snails as Determined by High Performance Thin-Layer Chromatography-Densitometry. Chromatography 2015, 2, 188-194. https://doi.org/10.3390/chromatography2020188
DeGrandchamp D, Hartlaub S, Fried B, Sherma J. Effects of Different Levels of Echinostoma caproni Miracidial Dose on Glucose and Maltose Composition of Biomphalaria glabrata Snails as Determined by High Performance Thin-Layer Chromatography-Densitometry. Chromatography. 2015; 2(2):188-194. https://doi.org/10.3390/chromatography2020188
Chicago/Turabian StyleDeGrandchamp, Dolcie, Sage Hartlaub, Bernard Fried, and Joseph Sherma. 2015. "Effects of Different Levels of Echinostoma caproni Miracidial Dose on Glucose and Maltose Composition of Biomphalaria glabrata Snails as Determined by High Performance Thin-Layer Chromatography-Densitometry" Chromatography 2, no. 2: 188-194. https://doi.org/10.3390/chromatography2020188