Next Article in Journal
Quantification of Carbonyl Compounds Generated from Ozone-Based Food Colorants Decomposition Using On-Fiber Derivatization-SPME-GC-MS
Next Article in Special Issue
Multivariate DoE Optimization of Asymmetric Flow Field Flow Fractionation Coupled to Quantitative LC-MS/MS for Analysis of Lipoprotein Subclasses
Previous Article in Journal
Electrophoretic Extraction and Proteomic Characterization of Proteins Buried in Marine Sediments
Previous Article in Special Issue
Cu Purification Using an Extraction Resin for Determination of Isotope Ratios by Multicollector ICP-MS
Article Menu

Export Article

From the first issue of 2016, Chromatography has changed its name to Separations.

Open AccessArticle
Chromatography 2014, 1(4), 211-226; doi:10.3390/chromatography1040211

Analyses of Phytohormones in Coconut (Cocos Nucifera L.) Water Using Capillary Electrophoresis-Tandem Mass Spectrometry

1
Natural Sciences and Science Education Academic Group, Nanyang Technological University, 1 Nanyang Walk, Singapore 637616, Singapore
2
Singapore University of Technology and Design, 20 Dover Road, Singapore 279623, Singapore
3
Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore
*
Author to whom correspondence should be addressed.
Received: 8 October 2014 / Revised: 8 December 2014 / Accepted: 12 December 2014 / Published: 22 December 2014
(This article belongs to the Special Issue Advances in Hyphenated Methods in Separation)
View Full-Text   |   Download PDF [1228 KB, uploaded 22 December 2014]   |  

Abstract

Capillary electrophoresis (CE) coupled with mass spectrometry (MS) or tandem mass spectrometry (MS/MS) is reported as an alternative and potentially useful method for the simultaneous analysis of various classes of phytohormones with diversified structures, including indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), abscisic acid (ABA), gibberellic acid (GA), zeatin (Z), N6-benzyladenine (BA), α-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). The key to the CE-MS/MS analysis was based on electroosmotic flow reversal using a cationic polymer-coated capillary. Under optimum conditions, a baseline separation of eight phytohormones was accomplished within 30 min using 60 mM ammonium formate/formic acid buffer of pH 3.8 with −20 kV as the separation voltage. The accessibility of MS/MS together with the characterization by migration properties obtained by CE allows for the development of CE-MS/MS as an emerging potential method for the analysis of different classes of phytohormones in a single run. The utility of the CE-MS/MS method was demonstrated by the comprehensive screening of phytohormones in coconut (Cocos nucifera L.) water after pre-concentration and purification through solid-phase extraction (SPE) cartridge. IAA, ABA, GA and Z were detected and quantified in the purified coconut water extract sample. View Full-Text
Keywords: capillary electrophoresis; tandem mass spectrometry; phytohormones; cationic polymer-coated capillary; solid-phase extraction capillary electrophoresis; tandem mass spectrometry; phytohormones; cationic polymer-coated capillary; solid-phase extraction
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Tan, S.N.; Yong, J.W.H.; Ge, L. Analyses of Phytohormones in Coconut (Cocos Nucifera L.) Water Using Capillary Electrophoresis-Tandem Mass Spectrometry. Chromatography 2014, 1, 211-226.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Chromatography EISSN 2227-9075 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top