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From the first issue of 2016, Chromatography has changed its name to Separations.

Open AccessArticle
Chromatography 2014, 1(4), 194-210; doi:10.3390/chromatography1040194

Description of the Retention and Peak Profile for Chromolith Columns in Isocratic and Gradient Elution Using Mobile Phase Composition and Flow Rate as Factors

Departament de Química Analítica, Universitat de València, c/Dr. Moliner 50, 46100 Burjassot, Spain
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Received: 22 October 2014 / Revised: 13 November 2014 / Accepted: 14 November 2014 / Published: 19 November 2014
(This article belongs to the Special Issue Monolithic Columns in Separation Sciences)
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Abstract

The effect of the modifier concentration and flow rate on the chromatographic performance of a second generation Chromolith® RP-18e column, under isocratic and gradient elution with acetonitrile-water mixtures, was examined using four sulphonamides as probe compounds. The acetonitrile concentration was varied between 5 and 55% (v/v), and the flow rate between 0.1 and 5.0 mL/min, keeping the other factors constant. The changes in both retention and peak profile were modelled, and used to build simple plots, where the logarithm of the retention factor was represented against the modifier concentration (in gradient elution, against the initial modifier concentration), and the half-widths or widths against the retention time (in gradient elution, against the time at the column outlet). A particular plot was needed for describing the retention of each sulphonamide, but due to the similar interaction kinetics, a unique plot described the changes in the half-widths for all four sulphonamides. The changes in retention with the flow showed that allegedly in the second generation Chromolith, the column deformation observed for the first generation Chromolith, with the applied pressure at increasing flow, is decreased. View Full-Text
Keywords: reversed-phase liquid chromatography; Chromolith; isocratic elution; gradient elution; flow programming; modelling reversed-phase liquid chromatography; Chromolith; isocratic elution; gradient elution; flow programming; modelling
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Cabo-Calvet, E.; Ortiz-Bolsico, C.; Baeza-Baeza, J.J.; García-Alvarez-Coque, M.C. Description of the Retention and Peak Profile for Chromolith Columns in Isocratic and Gradient Elution Using Mobile Phase Composition and Flow Rate as Factors. Chromatography 2014, 1, 194-210.

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