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Proteomes 2016, 4(1), 2; doi:10.3390/proteomes4010002

Will Quantitative Proteomics Redefine Some of the Key Concepts in Skeletal Muscle Physiology?

Department of Animal Molecular Physiology, Wroclaw University, Cybulskiego 30, 50-205 Wroclaw, Poland
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Author to whom correspondence should be addressed.
Academic Editors: Jacek R. Wisniewski and Jatin G. Burniston
Received: 15 October 2015 / Revised: 21 December 2015 / Accepted: 5 January 2016 / Published: 11 January 2016
(This article belongs to the Special Issue Striated Muscle Proteomics)
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Abstract

Molecular and cellular biology methodology is traditionally based on the reasoning called “the mechanistic explanation”. In practice, this means identifying and selecting correlations between biological processes which result from our manipulation of a biological system. In theory, a successful application of this approach requires precise knowledge about all parameters of a studied system. However, in practice, due to the systems’ complexity, this requirement is rarely, if ever, accomplished. Typically, it is limited to a quantitative or semi-quantitative measurements of selected parameters (e.g., concentrations of some metabolites), and a qualitative or semi-quantitative description of expression/post-translational modifications changes within selected proteins. A quantitative proteomics approach gives a possibility of quantitative characterization of the entire proteome of a biological system, in the context of the titer of proteins as well as their post-translational modifications. This enables not only more accurate testing of novel hypotheses but also provides tools that can be used to verify some of the most fundamental dogmas of modern biology. In this short review, we discuss some of the consequences of using quantitative proteomics to verify several key concepts in skeletal muscle physiology. View Full-Text
Keywords: C2C12 cells; kcat; glyconeogenesis; glycolysis regulation; lysine acetylation; skeletal muscle C2C12 cells; kcat; glyconeogenesis; glycolysis regulation; lysine acetylation; skeletal muscle
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Gizak, A.; Rakus, D. Will Quantitative Proteomics Redefine Some of the Key Concepts in Skeletal Muscle Physiology? Proteomes 2016, 4, 2.

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