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Proteomes 2016, 4(1), 1; doi:10.3390/proteomes4010001

Quantitative Proteomic Analysis of the Response to Zinc, Magnesium, and Calcium Deficiency in Specific Cell Types of Arabidopsis Roots

1
Department of Bioinformatics, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan
2
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma 630-0192, Japan
3
Centre for Plant Biotechnology, Division of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar, Jammu and Kashmir 190025, India
4
Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan
5
Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Ganesh Kumar Agrawal, Sun Tae Kim and Randeep Rakwal
Received: 22 October 2015 / Revised: 14 December 2015 / Accepted: 23 December 2015 / Published: 12 January 2016
(This article belongs to the Special Issue Advances in Plant Proteomics: Methodology to Biology)
View Full-Text   |   Download PDF [1360 KB, uploaded 12 January 2016]   |  

Abstract

The proteome profiles of specific cell types have recently been investigated using techniques such as fluorescence activated cell sorting and laser capture microdissection. However, quantitative proteomic analysis of specific cell types has not yet been performed. In this study, to investigate the response of the proteome to zinc, magnesium, and calcium deficiency in specific cell types of Arabidopsis thaliana roots, we performed isobaric tags for relative and absolute quantification (iTRAQ)-based quantitative proteomics using GFP-expressing protoplasts collected by fluorescence-activated cell sorting. Protoplasts were collected from the pGL2-GFPer and pMGP-GFPer marker lines for epidermis or inner cell lines (pericycle, endodermis, and cortex), respectively. To increase the number of proteins identified, iTRAQ-labeled peptides were separated into 24 fractions by OFFGFEL electrophoresis prior to high-performance liquid chromatography coupled with mass spectrometry analysis. Overall, 1039 and 737 proteins were identified and quantified in the epidermal and inner cell lines, respectively. Interestingly, the expression of many proteins was decreased in the epidermis by mineral deficiency, although a weaker effect was observed in inner cell lines such as the pericycle, endodermis, and cortex. Here, we report for the first time the quantitative proteomics of specific cell types in Arabidopsis roots. View Full-Text
Keywords: FACS; iTRAQ; OFFGEL electrophoresis; root specific cell types; 14-3-3 protein family; Arabidopsis thaliana FACS; iTRAQ; OFFGEL electrophoresis; root specific cell types; 14-3-3 protein family; Arabidopsis thaliana
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Fukao, Y.; Kobayashi, M.; Zargar, S.M.; Kurata, R.; Fukui, R.; Mori, I.C.; Ogata, Y. Quantitative Proteomic Analysis of the Response to Zinc, Magnesium, and Calcium Deficiency in Specific Cell Types of Arabidopsis Roots. Proteomes 2016, 4, 1.

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