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Proteomes 2014, 2(2), 169-190; doi:10.3390/proteomes2020169
Article

Effect of Aluminum Treatment on Proteomes of Radicles of Seeds Derived from Al-Treated Tomato Plants

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Received: 18 February 2014; in revised form: 4 March 2014 / Accepted: 6 March 2014 / Published: 28 March 2014
(This article belongs to the Special Issue Plant Proteomics)
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Abstract: Aluminum (Al) toxicity is a major constraint to plant growth and crop yield in acid soils. Tomato cultivars are especially susceptible to excessive Al3+ accumulated in the root zone. In this study, tomato plants were grown in a hydroponic culture system supplemented with 50 µM AlK(SO4)2. Seeds harvested from Al-treated plants contained a significantly higher Al content than those grown in the control hydroponic solution. In this study, these Al-enriched tomato seeds (harvested from Al-treated tomato plants) were germinated in 50 µM AlK(SO4)2 solution in a homopiperazine-1,4-bis(2-ethanesulfonic acid) buffer (pH 4.0), and the control solution which contained the buffer only. Proteomes of radicles were analyzed quantitatively by mass spectrometry employing isobaric tags for relative and absolute quantitation (iTRAQ®). The proteins identified were assigned to molecular functional groups and cellular metabolic pathways using MapMan. Among the proteins whose abundance levels changed significantly were: a number of transcription factors; proteins regulating gene silencing and programmed cell death; proteins in primary and secondary signaling pathways, including phytohormone signaling and proteins for enhancing tolerance to abiotic and biotic stress. Among the metabolic pathways, enzymes in glycolysis and fermentation and sucrolytic pathways were repressed. Secondary metabolic pathways including the mevalonate pathway and lignin biosynthesis were induced. Biological reactions in mitochondria seem to be induced due to an increase in the abundance level of mitochondrial ribosomes and enzymes in the TCA cycle, electron transport chains and ATP synthesis.
Keywords: iTRAQ; orbitrap mass spectrometry; Al-enriched tomato seeds; seed germination; root proteomics; functional pathways iTRAQ; orbitrap mass spectrometry; Al-enriched tomato seeds; seed germination; root proteomics; functional pathways
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Okekeogbu, I.; Ye, Z.; Sangireddy, S.R.; Li, H.; Bhatti, S.; Hui, D.; Zhou, S.; Howe, K.J.; Fish, T.; Yang, Y.; Thannhauser, T.W. Effect of Aluminum Treatment on Proteomes of Radicles of Seeds Derived from Al-Treated Tomato Plants. Proteomes 2014, 2, 169-190.

AMA Style

Okekeogbu I, Ye Z, Sangireddy SR, Li H, Bhatti S, Hui D, Zhou S, Howe KJ, Fish T, Yang Y, Thannhauser TW. Effect of Aluminum Treatment on Proteomes of Radicles of Seeds Derived from Al-Treated Tomato Plants. Proteomes. 2014; 2(2):169-190.

Chicago/Turabian Style

Okekeogbu, Ikenna; Ye, Zhujia; Sangireddy, Sasikiran R.; Li, Hui; Bhatti, Sarabjit; Hui, Dafeng; Zhou, Suping; Howe, Kevin J.; Fish, Tara; Yang, Yong; Thannhauser, Theodore W. 2014. "Effect of Aluminum Treatment on Proteomes of Radicles of Seeds Derived from Al-Treated Tomato Plants." Proteomes 2, no. 2: 169-190.

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