Next Article in Journal
Previous Article in Journal
Proteomes 2014, 2(2), 191-207; doi:10.3390/proteomes2020191
Article

Developmentally-Dynamic Murine Brain Proteomes and Phosphoproteomes Revealed by Quantitative Proteomics

 and *
Received: 31 December 2013; in revised form: 14 March 2014 / Accepted: 26 March 2014 / Published: 3 April 2014
View Full-Text   |   Download PDF [1797 KB, uploaded 3 April 2014]
Abstract: Developmental processes are governed by a diverse suite of signaling pathways employing reversible phosphorylation. Recent advances in large-scale phosphoproteomic methodologies have made possible the identification and quantification of hundreds to thousands of phosphorylation sites from primary tissues. Towards a global characterization of proteomic changes across brain development, we present the results of a large-scale quantitative mass spectrometry study comparing embryonic, newborn and adult murine brain. Using anti-phosphotyrosine immuno-affinity chromatography and strong cation exchange (SCX) chromatography, coupled to immobilized metal affinity chromatography (IMAC), we identified and quantified over 1,750 phosphorylation sites and over 1,300 proteins between three developmental states. Bioinformatic analyses highlight functions associated with the identified proteins and phosphoproteins and their enrichment at distinct developmental stages. These results serve as a primary reference resource and reveal dynamic developmental profiles of proteins and phosphoproteins from the developing murine brain.
Keywords: brain development; phosphoproteomics; phosphorylation; quantitative mass spectrometry; reductive amination brain development; phosphoproteomics; phosphorylation; quantitative mass spectrometry; reductive amination
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Doubleday, P.F.; Ballif, B.A. Developmentally-Dynamic Murine Brain Proteomes and Phosphoproteomes Revealed by Quantitative Proteomics. Proteomes 2014, 2, 191-207.

AMA Style

Doubleday PF, Ballif BA. Developmentally-Dynamic Murine Brain Proteomes and Phosphoproteomes Revealed by Quantitative Proteomics. Proteomes. 2014; 2(2):191-207.

Chicago/Turabian Style

Doubleday, Peter F.; Ballif, Bryan A. 2014. "Developmentally-Dynamic Murine Brain Proteomes and Phosphoproteomes Revealed by Quantitative Proteomics." Proteomes 2, no. 2: 191-207.

Proteomes EISSN 2227-7382 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert