Plants 2014, 3(1), 177-195; doi:10.3390/plants3010177
Article

Accumulation of TIP2;2 Aquaporin during Dark Adaptation Is Partially PhyA Dependent in Roots of Arabidopsis Seedlings

1,†email, 2,‡, 3email, 1,§email, 1email, 3email and 2,* email
Received: 15 November 2013; in revised form: 2 February 2014 / Accepted: 21 February 2014 / Published: 5 March 2014
(This article belongs to the Special Issue Plant Light Signalling)
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Abstract: Light regulates the expression and function of aquaporins, which are involved in water and solute transport. In Arabidopsis thaliana, mRNA levels of one of the aquaporin genes, TIP2;2, increase during dark adaptation and decrease under far-red light illumination, but the effects of light at the protein level and on the mechanism of light regulation remain unknown. Numerous studies have described the light regulation of aquaporin genes, but none have identified the regulatory mechanisms behind this regulation via specific photoreceptor signaling. In this paper, we focus on the role of phytochrome A (phyA) signaling in the regulation of the TIP2;2 protein. We generated Arabidopsis transgenic plants expressing a TIP2;2-GFP fusion protein driven by its own promoter, and showed several differences in TIP2;2 behavior between wild type and the phyA mutant. Fluorescence of TIP2;2-GFP protein in the endodermis of roots in the wild-type seedlings increased during dark adaptation, but not in the phyA mutant. The amount of the TIP2;2-GFP protein in wild-type seedlings decreased rapidly under far-red light illumination, and a delay in reduction of TIP2;2-GFP was observed in the phyA mutant. Our results imply that phyA, cooperating with other photoreceptors, modulates the level of TIP2;2 in Arabidopsis roots.
Keywords: Arabidopsis thaliana; aquaporin; dark adaptation; endodermis; light regulation; phytochrome; root; water transport
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Uenishi, Y.; Nakabayashi, Y.; Tsuchihira, A.; Takusagawa, M.; Hashimoto, K.; Maeshima, M.; Sato-Nara, K. Accumulation of TIP2;2 Aquaporin during Dark Adaptation Is Partially PhyA Dependent in Roots of Arabidopsis Seedlings. Plants 2014, 3, 177-195.

AMA Style

Uenishi Y, Nakabayashi Y, Tsuchihira A, Takusagawa M, Hashimoto K, Maeshima M, Sato-Nara K. Accumulation of TIP2;2 Aquaporin during Dark Adaptation Is Partially PhyA Dependent in Roots of Arabidopsis Seedlings. Plants. 2014; 3(1):177-195.

Chicago/Turabian Style

Uenishi, Yumi; Nakabayashi, Yukari; Tsuchihira, Ayako; Takusagawa, Mari; Hashimoto, Kayo; Maeshima, Masayoshi; Sato-Nara, Kumi. 2014. "Accumulation of TIP2;2 Aquaporin during Dark Adaptation Is Partially PhyA Dependent in Roots of Arabidopsis Seedlings." Plants 3, no. 1: 177-195.

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