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Plants 2013, 2(4), 750-768; doi:10.3390/plants2040750
Article

Experimental Measurements and Mathematical Modeling of Cytosolic Ca2+ Signatures upon Elicitation by Penta-N-acetylchitopentaose Oligosaccharides in Nicotiana tabacum Cell Cultures

,
 and
*
Department of Proteome and Metabolome Research, Faculty of Biology, Bielefeld University, Universitätsstr. 25, Bielefeld 33615, Germany
* Author to whom correspondence should be addressed.
Received: 29 September 2013 / Revised: 4 November 2013 / Accepted: 8 November 2013 / Published: 27 November 2013
(This article belongs to the Special Issue Calcium Signaling in Plants)
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Abstract

Plants have developed sophisticated recognition systems for different kinds of pathogens. Pathogen-associated molecular patterns (PAMPs) can induce various defense mechanisms, e.g., the production of reactive oxygen species (ROS) as an early event. Plant defense reactions are initiated by a signal transduction cascade involving the release of calcium ions (Ca2+) from both external and internal stores to the plant cytoplasm. This work focuses on the analysis of cytosolic Ca2+ signatures, experimentally and theoretically. Cytosolic Ca2+ signals were measured in Nicotiana tabacum plant cell cultures after elicitation with penta-N-acetylchitopentaose oligosaccharides (Ch5). In order to allow a mathematical simulation of the elicitor-triggered Ca2+ release, the Li and Rinzel model was adapted to the situation in plants. The main features of the Ca2+ response, like the specific shape of the Ca2+ transient and the dose-response relationship, could be reproduced very well. Repeated elicitation of the same cell culture revealed a refractory behavior with respect to the Ca2+ transients for this condition. Detailed analysis of the obtained data resulted in further modifications of the mathematical model, allowing a predictive simulation of Ch5-induced Ca2+ transients. The promising results may contribute to a deeper understanding of the underlying mechanisms governing plant defense.
Keywords: plant defense; calcium; modeling plant defense; calcium; modeling
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Mrozek, K.; Niehaus, K.; Lutter, P. Experimental Measurements and Mathematical Modeling of Cytosolic Ca2+ Signatures upon Elicitation by Penta-N-acetylchitopentaose Oligosaccharides in Nicotiana tabacum Cell Cultures. Plants 2013, 2, 750-768.

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