Next Article in Journal
Experimental Measurements and Mathematical Modeling of Cytosolic Ca2+ Signatures upon Elicitation by Penta-N-acetylchitopentaose Oligosaccharides in Nicotiana tabacum Cell Cultures
Next Article in Special Issue
Cellular Auxin Transport in Algae
Previous Article in Journal
Responses of African Grasses in the Genus Sporobolus to Defoliation and Sodium Stress: Tradeoffs, Cross-Tolerance, or Independent Responses?
Article Menu

Export Article

Open AccessArticle
Plants 2013, 2(4), 726-749; doi:10.3390/plants2040726

The Clubroot Pathogen (Plasmodiophora brassicae) Influences Auxin Signaling to Regulate Auxin Homeostasis in Arabidopsis

1
Institut für Botanik, Technische Universität Dresden, 01062 Dresden, Germany
2
Department of Agronomy and Horticulture, University of Nebraska, 379 Keim, Lincoln, NE 68521 USA
3
Botanisches Institut, Universität Kiel, Am Botanischen Garten 5, 24118 Kiel, Germany
These authors contributed equally to this work.
Present address: Humboldt Universität, 10099 Berlin, Germany.
*
Author to whom correspondence should be addressed.
Received: 16 September 2013 / Revised: 17 October 2013 / Accepted: 18 November 2013 / Published: 27 November 2013
(This article belongs to the Special Issue Auxin Signaling, Transport, and Metabolism)
View Full-Text   |   Download PDF [1503 KB, uploaded 27 November 2013]   |  

Abstract

The clubroot disease, caused by the obligate biotrophic protist Plasmodiophora brassicae, affects cruciferous crops worldwide. It is characterized by root swellings as symptoms, which are dependent on the alteration of auxin and cytokinin metabolism. Here, we describe that two different classes of auxin receptors, the TIR family and the auxin binding protein 1 (ABP1) in Arabidopsis thaliana are transcriptionally upregulated upon gall formation. Mutations in the TIR family resulted in more susceptible reactions to the root pathogen. As target genes for the different pathways we have investigated the transcriptional regulation of selected transcriptional repressors (Aux/IAA) and transcription factors (ARF). As the TIR pathway controls auxin homeostasis via the upregulation of some auxin conjugate synthetases (GH3), the expression of selected GH3 genes was also investigated, showing in most cases upregulation. A double gh3 mutant showed also slightly higher susceptibility to P. brassicae infection, while all tested single mutants did not show any alteration in the clubroot phenotype. As targets for the ABP1-induced cell elongation the effect of potassium channel blockers on clubroot formation was investigated. Treatment with tetraethylammonium (TEA) resulted in less severe clubroot symptoms. This research provides evidence for the involvement of two auxin signaling pathways in Arabidopsis needed for the establishment of the root galls by P. brassicae. View Full-Text
Keywords: ABP1; Arabidopsis thaliana; auxin homeostasis; auxin receptors; clubroot disease; GH3 proteins; Plasmodiophora brassicae; potassium channel inhibitors; tetraethylammonium; TIR1 ABP1; Arabidopsis thaliana; auxin homeostasis; auxin receptors; clubroot disease; GH3 proteins; Plasmodiophora brassicae; potassium channel inhibitors; tetraethylammonium; TIR1
Figures

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Jahn, L.; Mucha, S.; Bergmann, S.; Horn, C.; Staswick, P.; Steffens, B.; Siemens, J.; Ludwig-Müller, J. The Clubroot Pathogen (Plasmodiophora brassicae) Influences Auxin Signaling to Regulate Auxin Homeostasis in Arabidopsis. Plants 2013, 2, 726-749.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Plants EISSN 2223-7747 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top