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Identification of Chimeric Repressors that Confer Salt and Osmotic Stress Tolerance in Arabidopsis
Graduate School of Bionics, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan
School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-0053, Japan
Institute for Environmental Science and Technology, Saitama University, Saitama-shi, Saitama 338-8570, Japan
* Author to whom correspondence should be addressed.
Received: 23 September 2013; in revised form: 9 October 2013 / Accepted: 22 November 2013 / Published: 5 December 2013
Abstract: We produced transgenic Arabidopsis plants that express chimeric genes for transcription factors converted to dominant repressors, using Chimeric REpressor gene-Silencing Technology (CRES-T), and evaluated the salt tolerance of each line. The seeds of the CRES-T lines for ADA2b, Msantd, DDF1, DREB26, AtGeBP, and ATHB23 exhibited higher germination rates than Wild type (WT) and developed rosette plants under up to 200 mM NaCl or 400 mM mannitol. WT plants did not grow under these conditions. In these CRES-T lines, the expression patterns of stress-related genes such as RD29A, RD22, DREB1A, and P5CS differed from those in WT plants, suggesting the involvement of the six transcription factors identified here in the stress response pathways regulated by the products of these stress-related genes. Our results demonstrate additional proof that CRES-T is a superior tool for revealing the function of transcription factors.
Keywords: CRES-T; osmotic stress; repressor; salt stress; stress tolerance; transcription factors
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Kazama, D.; Itakura, M.; Kurusu, T.; Mitsuda, N.; Ohme-Takagi, M.; Tada, Y. Identification of Chimeric Repressors that Confer Salt and Osmotic Stress Tolerance in Arabidopsis. Plants 2013, 2, 769-785.
Kazama D, Itakura M, Kurusu T, Mitsuda N, Ohme-Takagi M, Tada Y. Identification of Chimeric Repressors that Confer Salt and Osmotic Stress Tolerance in Arabidopsis. Plants. 2013; 2(4):769-785.
Kazama, Daisuke; Itakura, Masateru; Kurusu, Takamitsu; Mitsuda, Nobutaka; Ohme-Takagi, Masaru; Tada, Yuichi. 2013. "Identification of Chimeric Repressors that Confer Salt and Osmotic Stress Tolerance in Arabidopsis." Plants 2, no. 4: 769-785.