Combinations of Mutations Sufficient to Alter Arabidopsis Leaf Dissection
Abstract
:1. Introduction
2. Results
2.1. Leaf Phenotype of Jaw-D, Stip-D, Mir164a-4/CUC2g-m4 Double and Triple Mutants
2.1.1. Jaw-D Stip-D Double Mutant Leaf Phenotype
2.1.2. Jaw-D Mir164a-4 and Jaw-D CUC2g-m4 Double Mutant Leaf Phenotype
2.1.3. Stip-D Mir164a-4 and Stip-D CUC2g-m4 Double Mutant Leaf Phenotype
2.1.4. jaw-D stip-D mir164a-4 and jaw-D stip-D CUC2g-m4 Triple Mutant Leaf Phenotype
2.1.5. Stip-D Promotes the Formation of Petiole
2.2. Floral Phenotype of jaw-D, stip-D, mir164a-4/CUC2g-m4 Double and Triple Mutants
2.3. Leaf Phenotype of TCP2 and TCP4 Insertion Lines
2.4. Expression of KNOX I Reporters
STM:GUS | KNAT1:GUS | KNAT2:GUS | KNAT6:GUS | |
---|---|---|---|---|
tips | ||||
WT | N.D. | N.D. | N.D. | |
trichomes | ||||
tips | ||||
CUC2g-m4 | N.D. | N.D. | N.D. | sinus |
trichomes | ||||
tips | ||||
stip-D | N.D. | N.D. | N.D. | sinus |
trichomes | ||||
tips | ||||
jaw-D | N.D. | N.D. | N.D. | sinus |
trichomes | ||||
tips | tips | |||
jaw-D stip-D | N.D. | N.D. | sinus | |
trichomes | ||||
tips | tips | |||
stip-D CUC2g-m4 | N.D. | sinus | N.D. | sinus |
trichomes | ||||
tips | tips | |||
jaw-D CUC2g-m4 | sinus | sinus | N.D. | sinus |
petiole | vasculature | trichomes | ||
N.D.: Not Detected |
3. Discussion
4. Experimental Section
4.1. Plant Material
4.2. GUS Assay
4.3. Scanning Electron Microscopy
5. Conclusions
Acknowledgments
References
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Blein, T.; Pautot, V.; Laufs, P. Combinations of Mutations Sufficient to Alter Arabidopsis Leaf Dissection. Plants 2013, 2, 230-247. https://doi.org/10.3390/plants2020230
Blein T, Pautot V, Laufs P. Combinations of Mutations Sufficient to Alter Arabidopsis Leaf Dissection. Plants. 2013; 2(2):230-247. https://doi.org/10.3390/plants2020230
Chicago/Turabian StyleBlein, Thomas, Véronique Pautot, and Patrick Laufs. 2013. "Combinations of Mutations Sufficient to Alter Arabidopsis Leaf Dissection" Plants 2, no. 2: 230-247. https://doi.org/10.3390/plants2020230