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PiSCP1 and PiCDPK2 Localize to Peroxisomes and Are Involved in Pollen Tube Growth in Petunia Inflata
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
Department of Biology University of North Carolina, Chapel Hill, NC 27599, USA
School of Biological Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA 99164, USA
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 6 January 2013; in revised form: 19 February 2013 / Accepted: 26 February 2013 / Published: 4 March 2013
Abstract: Petunia inflata small CDPK-interacting protein 1 (PiSCP1) was identified as a pollen expressed PiCDPK1 interacting protein using the yeast two hybrid system and the interaction confirmed using pull-down and phosphorylation assays. PiSCP1 is pollen specific and shares amino acid homology with uncharacterized proteins from diverse species of higher plants, but no protein of known function. Expression of PiSCP1-GFP in vivo inhibited pollen tube growth and was shown to localize to peroxisomes in growing pollen tubes. As PiCDPK1 is plasma membrane localized, we investigated the localization of a second isoform, PiCDPK2, and show that it co-localizes to peroxisomes with PiSCP1 and that the two proteins interact in the yeast 2 hybrid interaction assay, suggesting that interaction with the latter CDPK isoform is likely the one of biological relevance. Both PiCDPK2 and PiSCP1 affect pollen tube growth, presumably by mediating peroxisome function, however how they do so is currently not clear.
Keywords: pollen tube growth; calcium dependent protein kinase; peroxisome
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Cite This Article
MDPI and ACS Style
Guo, F.; Yoon, G.M.; McCubbin, A.G. PiSCP1 and PiCDPK2 Localize to Peroxisomes and Are Involved in Pollen Tube Growth in Petunia Inflata. Plants 2013, 2, 72-86.
Guo F, Yoon GM, McCubbin AG. PiSCP1 and PiCDPK2 Localize to Peroxisomes and Are Involved in Pollen Tube Growth in Petunia Inflata. Plants. 2013; 2(1):72-86.
Guo, Feng; Yoon, Gyeong M.; McCubbin, Andrew G. 2013. "PiSCP1 and PiCDPK2 Localize to Peroxisomes and Are Involved in Pollen Tube Growth in Petunia Inflata." Plants 2, no. 1: 72-86.