Epicardium-Derived Heart Repair
Abstract
:1. Cardiovascular Disease
2. Cardiac Regeneration
3. The Epicardium as a Source of Endogenous Progenitor Cells
4. Mouse Models to Identify Epicardial Cells in Vivo
5. The Post-Natal Epicardium
5.1. The Intact Heart
5.2. The Injured Adult Heart
6. Epicardium Derived Cell Differentiation into Cardiovascular Cell Types
6.1. Cardiomyocyte Formation
6.2. Neovascularisation
6.3. Fibrotic Response
7. Discussion
Model | Activation | EPDC Differentiation | Markers Used for Identification | Time-points | Details | Reference |
---|---|---|---|---|---|---|
WT1CreERT2/+; R26RmTmG | Expansion | SMC Fibro Sporadic EC | SM-MHC, αSMA, SM22α FN1, ColIII, FSP1, ProCol1 PECAM | 14d # | Tamoxifen: twice weekly for 2-3 weeks, MI one week after final injection | 33 |
Ad:Msln-Cre; R26RmTmG | n.a. | Fibro | FSP1 | 3d-4wks | Ultrasound-guided virus delivery | 33 |
WT1CreERT2/+; R26REYFP | Expansion Migration | CM* | cTnT, sαActin, Cx43, N-Cad, Ca2+ transients Functional coupling | 14d | Tβ4: Pre- and post-MI Tamoxifen: 5 and 3 days pre- MI | 34 |
Gata5-Cre; R26REYFP | Expansion Migration | SMC$ EC$ Fibro | αSMA PECAM ProColI | 7d | Tβ4: Pre- and post-MI | 36 |
WT1CreERT2/+; R26RmTmG | Expansion | SMC Fibro | αSMA DDR2, ProCol1, desmin, FSP1, ColIII | 14d | Tamoxifen: twice weekly for 2-3 weeks, MI one week after final injection Tβ4: Post-MI | 38 |
(BAC)WT1EGFPCre; R26R | Expansion Migration | SMC EC CM^ Fibro | αSMA PECAM + location in vessel wall cTnI, SERCA2 via exclusion of other markers | 1mo, 3mo (CM) | 43 | |
LV-CMVGFP | Migration | CM* | sαActin, morphology | 7d, 21d | Sub-pericardial virus injection | 44 |
8. Conclusion
Acknowledgements
Author Contributions
Conflicts of Interest
References
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Smits, A.M.; Riley, P.R. Epicardium-Derived Heart Repair. J. Dev. Biol. 2014, 2, 84-100. https://doi.org/10.3390/jdb2020084
Smits AM, Riley PR. Epicardium-Derived Heart Repair. Journal of Developmental Biology. 2014; 2(2):84-100. https://doi.org/10.3390/jdb2020084
Chicago/Turabian StyleSmits, Anke M., and Paul R. Riley. 2014. "Epicardium-Derived Heart Repair" Journal of Developmental Biology 2, no. 2: 84-100. https://doi.org/10.3390/jdb2020084