Metal Binding Properties of the N-Terminus of the Functional Amyloid Orb2
AbstractThe cytoplasmic polyadenylation element binding protein (CPEB) homologue Orb2 is a functional amyloid that plays a key regulatory role for long-term memory in Drosophila. Orb2 has a glutamine, histidine-rich (Q/H-rich) domain that resembles the Q/H-rich, metal binding domain of the Hpn-like protein (Hpnl) found in Helicobacter pylori. In the present study, we used chromatography and isothermal titration calorimetry (ITC) to show that the Q/H-rich domain of Orb2 binds Ni2+ and other transition metals ions with μM affinity. Using site directed mutagenesis, we show that several histidine residues are important for binding. In particular, the H61Y mutation, which was previously shown to affect the aggregation of Orb2 in cell culture, completely inhibited metal binding of Orb2. Finally, we used thioflavin T fluorescence and electron microscopy images to show that Ni2+ binding induces the aggregating of Orb2 into structures that are distinct from the amyloid fibrils formed in the absence of Ni2+. These data suggest that transition metal binding might be important for the function of Orb2 and potentially long-term memory in Drosophila. View Full-Text
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Bajakian, T.H.; Cervantes, S.A.; Soria, M.A.; Beaugrand, M.; Kim, J.Y.; Service, R.J.; Siemer, A.B. Metal Binding Properties of the N-Terminus of the Functional Amyloid Orb2. Biomolecules 2017, 7, 57.
Bajakian TH, Cervantes SA, Soria MA, Beaugrand M, Kim JY, Service RJ, Siemer AB. Metal Binding Properties of the N-Terminus of the Functional Amyloid Orb2. Biomolecules. 2017; 7(3):57.Chicago/Turabian Style
Bajakian, Thalia H.; Cervantes, Silvia A.; Soria, Maria A.; Beaugrand, Maïwenn; Kim, Ji Yun; Service, Rachel J.; Siemer, Ansgar B. 2017. "Metal Binding Properties of the N-Terminus of the Functional Amyloid Orb2." Biomolecules 7, no. 3: 57.
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