Sphingosine Phosphate Lyase Regulates Murine Embryonic Stem Cell Proliferation and Pluripotency through an S1P2/STAT3 Signaling Pathway
Abstract
:1. Introduction
2. Results and Discussion
2.1. Silencing of SPL in mESCs
2.2. SPL Silencing Enhances mESC Proliferation and Pluripotency
2.3. SPL Silencing Acts via STAT3 Signaling to Enhance mESC Proliferation and Pluripotency
2.4. SPL Silencing Acts via S1P2 to Enhance mESC Proliferation and Pluripotency
2.5. S1P2 Activates STAT3 Signaling in mESCs
2.6. SPL-KD Cells are Capable of Generating Tissue Stem Cells
2.7. Discussion
3. Experimental Section
3.1. Embryonic Stem Cell Culture
3.2. SPL Knockdown
3.3. SPL Assay
3.4. Mass Spectrometry
3.5. Western Blotting
3.6. Cell Proliferation Assays
3.7. RT-PCR
3.8. Isolation of Satellite Cells from mESC Embryoid Bodies
3.9. Statistical Analysis
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Smith, G.S.; Kumar, A.; Saba, J.D. Sphingosine Phosphate Lyase Regulates Murine Embryonic Stem Cell Proliferation and Pluripotency through an S1P2/STAT3 Signaling Pathway. Biomolecules 2013, 3, 351-368. https://doi.org/10.3390/biom3030351
Smith GS, Kumar A, Saba JD. Sphingosine Phosphate Lyase Regulates Murine Embryonic Stem Cell Proliferation and Pluripotency through an S1P2/STAT3 Signaling Pathway. Biomolecules. 2013; 3(3):351-368. https://doi.org/10.3390/biom3030351
Chicago/Turabian StyleSmith, Gaelen S., Ashok Kumar, and Julie D. Saba. 2013. "Sphingosine Phosphate Lyase Regulates Murine Embryonic Stem Cell Proliferation and Pluripotency through an S1P2/STAT3 Signaling Pathway" Biomolecules 3, no. 3: 351-368. https://doi.org/10.3390/biom3030351