Metabolites 2014, 4(3), 599-611; doi:10.3390/metabo4030599
Metabolite Profiling of Root Exudates of Common Bean under Phosphorus Deficiency
Keitaro Tawaraya 1,*
, Ryota Horie 1, Saki Saito 1, Tadao Wagatsuma 1, Kazuki Saito 2
and Akira Oikawa 1,2
and Akira Oikawa 1,21
Faculty of Agriculture, Yamagata University, Tsuruoka 997-8555, Japan
2
RIKEN Center for Sustainable Resource Science, Yokohama 230-0045, Japan
*
Author to whom correspondence should be addressed.
Received: 24 April 2014 / Revised: 29 June 2014 / Accepted: 3 July 2014 / Published: 16 July 2014
(This article belongs to the Special Issue Metabolomics and Biotechnology)
Abstract
Root exudates improve the nutrient acquisition of plants and affect rhizosphere microbial communities. The plant nutrient status affects the composition of root exudates. The purpose of this study was to examine common bean (Phaseolus vulgaris L.) root exudates under phosphorus (P) deficiency using a metabolite profiling technique. Common bean plants were grown in a culture solution at P concentrations of 0 (P0), 1 (P1) and 8 (P8) mg P L−1 for 1, 10 and 20 days after transplanting (DAT). Root exudates were collected, and their metabolites were determined by capillary electrophoresis time-of-flight mass spectrometry (CE-TOF MS). The shoot P concentration and dry weight of common bean plants grown at P0 were lower than those grown at P8. One hundred and fifty-nine, 203 and 212 metabolites were identified in the root exudates, and 16% (26/159), 13% (26/203) and 9% (20/212) of metabolites showed a P0/P8 ratio higher than 2.0 at 1, 10 and 20 DAT, respectively. The relative peak areas of several metabolites, including organic acids and amino acids, in root exudates were higher at P0 than at P8. These results suggest that more than 10% of primary and secondary metabolites are induced to exude from roots of common bean by P deficiency. View Full-TextKeywords:
Phaseolus vulgaris; phosphorus deficiency; rhizosphere; root exudates
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Tawaraya, K.; Horie, R.; Saito, S.; Wagatsuma, T.; Saito, K.; Oikawa, A. Metabolite Profiling of Root Exudates of Common Bean under Phosphorus Deficiency. Metabolites 2014, 4, 599-611.
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