Metabolites 2014, 4(2), 499-516; doi:10.3390/metabo4020499
Article

Molar-Based Targeted Metabolic Profiling of Cyanobacterial Strains with Potential for Biological Production

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Received: 17 April 2014; in revised form: 5 June 2014 / Accepted: 12 June 2014 / Published: 20 June 2014
(This article belongs to the Special Issue Metabolomics and Biotechnology)
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Abstract: Recently, cyanobacteria have become one of the most attractive hosts for biochemical production due to its high proliferative ability and ease of genetic manipulation. Several researches aimed at biological production using modified cyanobacteria have been reported previously. However, to improve the yield of bioproducts, a thorough understanding of the intercellular metabolism of cyanobacteria is necessary. Metabolic profiling techniques have proven to be powerful tools for monitoring cellular metabolism of various organisms and can be applied to elucidate the details of cyanobacterial metabolism. In this study, we constructed a metabolic profiling method for cyanobacteria using 13C-labeled cell extracts as internal standards. Using this method, absolute concentrations of 84 metabolites were successfully determined in three cyanobacterial strains which are commonly used as background strains for metabolic engineering. By comparing the differences in basic metabolic potentials of the three cyanobacterial strains, we found a well-correlated relationship between intracellular energy state and growth in cyanobacteria. By integrating our results with the previously reported biological production pathways in cyanobacteria, we found putative limiting step of carbon flux. The information obtained from this study will not only help gain insights in cyanobacterial physiology but also serve as a foundation for future metabolic engineering studies using cyanobacteria.
Keywords: metabolomics; cyanobacteria; biological production; absolute quantitation; triple quadrupole mass spectrometry
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Dempo, Y.; Ohta, E.; Nakayama, Y.; Bamba, T.; Fukusaki, E. Molar-Based Targeted Metabolic Profiling of Cyanobacterial Strains with Potential for Biological Production. Metabolites 2014, 4, 499-516.

AMA Style

Dempo Y, Ohta E, Nakayama Y, Bamba T, Fukusaki E. Molar-Based Targeted Metabolic Profiling of Cyanobacterial Strains with Potential for Biological Production. Metabolites. 2014; 4(2):499-516.

Chicago/Turabian Style

Dempo, Yudai; Ohta, Erika; Nakayama, Yasumune; Bamba, Takeshi; Fukusaki, Eiichiro. 2014. "Molar-Based Targeted Metabolic Profiling of Cyanobacterial Strains with Potential for Biological Production." Metabolites 4, no. 2: 499-516.


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