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Fluxomics of the Eastern Oyster for Environmental Stress Studies
Joint Department of Biomedical Engineering, NC State University and UNC Chapel Hill, Chapel Hill, NC 27599, USA
Environmental Medicine Consortium, NC State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA
Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA
* Author to whom correspondence should be addressed.
Received: 21 August 2013; in revised form: 27 November 2013 / Accepted: 19 December 2013 / Published: 7 January 2014
Abstract: The metabolism of 2-13C/15N-glycine and U-13C-glucose was determined in four tissue blocks (adductor muscle, stomach and digestive gland, mantle, and gills) of the Eastern oyster (Crassostrea virginica) using proton (1H) and carbon-13 (13C) nuclear magnetic resonance (NMR) spectroscopy. The oysters were treated in aerated seawater with three treatments (5.5 mM U-13C-glucose, 2.7 mM 2-13C/15N-glycine, and 5.5 mM U-13C-glucose plus 2.7 mM 2-13C/15N-glycine) and the relative mass balance and 13C fractional enrichments were determined in the four tissue blocks. In all tissues, glycine was metabolized by the glycine cycle forming serine exclusively in the mitochondria by the glycine cleavage system forming 2,3-13C-serine. In muscle, a minor amount of serine-derived pyruvate entered the Krebs cycle as substantiated by detection of a trace of 2,3-13C-aspartate. In all tissues, U-13C-glucose formed glycogen by glycogen synthesis, alanine by glycolysis, and glutamate and aspartate through the Krebs cycle. Alanine was formed exclusively from glucose via alanine transaminase and not glycine via alanine-glyoxylate transaminase. Based on isotopomer analysis, pyruvate carboxylase and pyruvate dehydrogenase appeared to be equal points for pyruvate entry into the Krebs cycle. In the 5.5 mM U-13C-glucose plus 2.7 mM 2-13C/15N-glycine emergence treatment used to simulate 12 h of “low tide”, oysters accumulated more 13C-labeled metabolites, including both anaerobic glycolytic and aerobic Krebs cycle intermediates. The aerobic metabolites could be the biochemical result of the gaping behavior of mollusks during emergence. The change in tissue distribution and mass balance of 13C-labeled nutrients (U-13C-glucose and 2-13C/15N-glycine) provides the basis for a new quantitative fluxomic method for elucidating sub-lethal environmental effects in marine organisms called whole body mass balance phenotyping (WoMBaP).
Keywords: 1H & 13C NMR; metabolomic; fluxomic; oyster; mass balance; 2-13C/15N-glycine; U-13C-glucose
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MDPI and ACS Style
Tikunov, A.P.; Stoskopf, M.K.; Macdonald, J.M. Fluxomics of the Eastern Oyster for Environmental Stress Studies. Metabolites 2014, 4, 53-70.
Tikunov AP, Stoskopf MK, Macdonald JM. Fluxomics of the Eastern Oyster for Environmental Stress Studies. Metabolites. 2014; 4(1):53-70.
Tikunov, Andrey P.; Stoskopf, Michael K.; Macdonald, Jeffrey M. 2014. "Fluxomics of the Eastern Oyster for Environmental Stress Studies." Metabolites 4, no. 1: 53-70.