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Cosmetics 2014, 1(2), 82-93; doi:10.3390/cosmetics1020082
Article

Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry Measurement of Caffeine in Caffeine-Laced Pants and in Urine and Skin of a Pants User

1
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2
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1
 and
1,*
1 Department of Therapeutic Research and Medicines Evaluation, National Institute of Health, V.le Regina Elena 299, 00161 Rome, Italy 2 Centro Nazionale Organismo Notificato Dispositivi e Cosmetici (ONDICO), National Institute of Health, V.le Regina Elena 299, 00161 Rome, Italy
* Author to whom correspondence should be addressed.
Received: 31 January 2014 / Revised: 31 March 2014 / Accepted: 6 April 2014 / Published: 15 April 2014
(This article belongs to the Special Issue Analytical Methods for Quality Control of Cosmetics)
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Abstract

A fast and sensitive ultra-performance liquid chromatography tandem mass spectrometry method was developed for the measurement of caffeine in caffeine-laced pants and in urine and skin of a pants user. The substance and its internal standard (N-ethylnorcotinine) were separated by reversed phase chromatography with 5 mM ammonium formate pH 3.0 and 0.3% formic acid in acetonitrile mobile phase (83:17 v/v) by isocratic elution and detected by tandem mass spectrometry operated in multiple reaction monitoring mode via positive electrospray ionization. Linearity was studied from 1.4 to100 ng/mL range for urine, from 5 to 100 ng/cotton swab for skin caffeine and from 1.3 to 100 µg/samples for 4 cm2 textile samples. Good determination coefficients (r2 = 0.99) were found in all cases. At three concentrations spanning the linear dynamic ranges of different samples mean recoveries of caffeine were always higher than 80% and intra-assay and inter-assay imprecision and inaccuracy were always better than 105%. For the first time, caffeine content in this cosmetotextile was determined together with the measurement of caffeine released on the user skin, the absorbed amount with resulting urinary concentrations.
Keywords: cosmetotextile; caffeine laced pants; ultra-performance liquid chromatography; tandem mass spectrometry cosmetotextile; caffeine laced pants; ultra-performance liquid chromatography; tandem mass spectrometry
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Pellegrini, M.; De Orsi, D.; Guarino, C.; Rotolo, M.C.; di Giovannandrea, R.; Pacifici, R.; Pichini, S. Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry Measurement of Caffeine in Caffeine-Laced Pants and in Urine and Skin of a Pants User. Cosmetics 2014, 1, 82-93.

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