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Antibiotics 2018, 7(1), 21; https://doi.org/10.3390/antibiotics7010021

Protein Expression Modifications in Phage-Resistant Mutants of Aeromonas salmonicida after AS-A Phage Treatment

1
Departament of Biology & CESAM, Campus Universitário de Santiago, Universidade de Aveiro, 3810-193 Aveiro, Portugal
2
Escola Superior de Tecnologia da Saúde, Rua 5 de Outubro, SM Bispo. Instituto Politécnico de Coimbra, Apartado 7006, 3046-854 Coimbra, Portugal
3
Departament of Chemistry, QOPNA, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 31 January 2018 / Revised: 1 March 2018 / Accepted: 6 March 2018 / Published: 8 March 2018
(This article belongs to the Special Issue Bacteriophages: Alternatives to Antibiotics and Beyond)
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Abstract

The occurrence of infections by pathogenic bacteria is one of the main sources of financial loss for the aquaculture industry. This problem often cannot be solved with antibiotic treatment or vaccination. Phage therapy seems to be an alternative environmentally-friendly strategy to control infections. Recognizing the cellular modifications that bacteriophage therapy may cause to the host is essential in order to confirm microbial inactivation, while understanding the mechanisms that drive the development of phage-resistant strains. The aim of this work was to detect cellular modifications that occur after phage AS-A treatment in A. salmonicida, an important fish pathogen. Phage-resistant and susceptible cells were subjected to five successive streak-plating steps and analysed with infrared spectroscopy, a fast and powerful tool for cell study. The spectral differences of both populations were investigated and compared with a phage sensitivity profile, obtained through the spot test and efficiency of plating. Changes in protein associated peaks were found, and these results were corroborated by 1-D electrophoresis of intracellular proteins analysis and by phage sensitivity profiles. Phage AS-A treatment before the first streaking-plate step clearly affected the intracellular proteins expression levels of phage-resistant clones, altering the expression of distinct proteins during the subsequent five successive streak-plating steps, making these clones recover and be phenotypically more similar to the sensitive cells. View Full-Text
Keywords: phage therapy; Aeromonas salmonicida; furunculosis; phage-resistant mutants; proteins; infrared spectroscopy phage therapy; Aeromonas salmonicida; furunculosis; phage-resistant mutants; proteins; infrared spectroscopy
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Moreirinha, C.; Osório, N.; Pereira, C.; Simões, S.; Delgadillo, I.; Almeida, A. Protein Expression Modifications in Phage-Resistant Mutants of Aeromonas salmonicida after AS-A Phage Treatment. Antibiotics 2018, 7, 21.

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