Next Article in Journal
A Point Prevalence Survey of Antibiotic Use in 18 Hospitals in Egypt
Previous Article in Journal
Acquired Class D β-Lactamases
Previous Article in Special Issue
Foreign Body Infection Models to Study Host-Pathogen Response and Antimicrobial Tolerance of Bacterial Biofilm
Antibiotics 2014, 3(3), 435-449; doi:10.3390/antibiotics3030435

A Novel Organo-Selenium Bandage that Inhibits Biofilm Development in a Wound by Gram-Positive and Gram-Negative Wound Pathogens

Received: 30 April 2014 / Revised: 7 August 2014 / Accepted: 19 August 2014 / Published: 25 August 2014
(This article belongs to the Special Issue Biofilm Infection)
View Full-Text   |   Download PDF [1807 KB, uploaded 25 August 2014]   |   Browse Figures


Biofilm formation in wounds is a serious problem which inhibits proper wound healing. One possible contributor to biofilm formation in a wound is the bacteria growing within the overlying bandage. To test this mechanism, we used bandages that contained a coating of organo-selenium that was covalently attached to the bandage. We tested the ability of this coating to kill bacteria on the bandage and in the underlying tissue. The bandage material was tested with both lab strains and clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa and Staphylococcus epidermidis. It was found that the organo-selenium coated bandage showed inhibition, of biofilm formation on the bandage in vitro (7–8 logs), with all the different bacteria tested, at selenium concentrations in the coating of less than 1.0%. These coatings were found to remain stable for over one month in aqueous solution, 15 min in boiling water, and over 6 years at room temperature. The bandages were also tested on a mouse wound model where the bacteria were injected between the bandage and the wound. Not only did the selenium bandage inhibit biofilm formation in the bandage, but it also inhibited biofilm formation in the wound tissue. Since selenium does not leave the bandage, this would appear to support the idea that a major player in wound biofilm formation is bacteria which grows in the overlying bandage.
Keywords: biofilm formation; wound healing; organo-selenium; bandage biofilm formation; wound healing; organo-selenium; bandage
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
MDPI and ACS Style

Tran, P.L.; Patel, S.; Hamood, A.N.; Enos, T.; Mosley, T.; Jarvis, C.; Desai, A.; Lin, P.; Reid, T.W. A Novel Organo-Selenium Bandage that Inhibits Biofilm Development in a Wound by Gram-Positive and Gram-Negative Wound Pathogens. Antibiotics 2014, 3, 435-449.

View more citation formats

Article Metrics


Cited By

[Return to top]
Antibiotics EISSN 2079-6382 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert