A Novel Organo-Selenium Bandage that Inhibits Biofilm Development in a Wound by Gram-Positive and Gram-Negative Wound Pathogens
Abstract
:1. Introduction
2. Results and Discussion
2.1. Organo-Selenium Bandage, Coated with Different Organo-Selenium Concentrations, was Tested for Its Ability to Block Biofilm Formation in Vitro for Laboratory Strains of Staphylococcus aureus and Pseudomonas aeruginosa
2.2. Confocal Visualization of the Biofilm Inhibition on a Bandage by Organo-Selenium in Vitro
2.3. Selenium Bandage Inhibition of Clinical Isolates of S. aureus, P. aeruginosa and S. epidermidis
2.4. Stability of the Selenium Bandage in Preventing Biofilm Formation
2.5. Stability of the Organo-Selenium Bandage to Boiling Water
2.6. In Vivo Study of the Ability of a 1% Selenium Coated Bandage to Inhibit Biofilm in Both the Bandage and the Underlying Wound
2.7. Mammalian Cell Toxicity Assay
3. Experimental
3.1. Coating Cellulose Gauze with Polymerized Se-AAEMA
3.2. Bacterial Strains
3.3. Colony-Forming Unit Assays—in Vitro
3.4. Mouse Wound Infection Model
3.5. Biofilm Analysis by Fluorescence Microscopy
3.6. Analysis of the Long-term Stability of the Organo-Selenium-Containing Gauze Bandage
3.7. Accessing the Stability of the Organo-Selenium-Containing Gauze Bandage at Elevated Temperature
3.8. Analysis of Selenium Toxicity to Mammalian Cells
3.9. Statistical Analysis
4. Conclusions
Author Contributions
Conflicts of Interest
References
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Tran, P.L.; Patel, S.; Hamood, A.N.; Enos, T.; Mosley, T.; Jarvis, C.; Desai, A.; Lin, P.; Reid, T.W. A Novel Organo-Selenium Bandage that Inhibits Biofilm Development in a Wound by Gram-Positive and Gram-Negative Wound Pathogens. Antibiotics 2014, 3, 435-449. https://doi.org/10.3390/antibiotics3030435
Tran PL, Patel S, Hamood AN, Enos T, Mosley T, Jarvis C, Desai A, Lin P, Reid TW. A Novel Organo-Selenium Bandage that Inhibits Biofilm Development in a Wound by Gram-Positive and Gram-Negative Wound Pathogens. Antibiotics. 2014; 3(3):435-449. https://doi.org/10.3390/antibiotics3030435
Chicago/Turabian StyleTran, Phat L., Saurabh Patel, Abdul N. Hamood, Tyler Enos, Thomas Mosley, Courtney Jarvis, Akash Desai, Pamela Lin, and Ted W. Reid. 2014. "A Novel Organo-Selenium Bandage that Inhibits Biofilm Development in a Wound by Gram-Positive and Gram-Negative Wound Pathogens" Antibiotics 3, no. 3: 435-449. https://doi.org/10.3390/antibiotics3030435