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Biosensors 2018, 8(2), 32; https://doi.org/10.3390/bios8020032

Development of a β-Lactoglobulin Sensor Based on SPR for Milk Allergens Detection

1
Advanced Diagnostics and Sensors Group, Cranfield University, Cranfield MK43 0AL, UK
2
Department of Micro- and Nanotechnology, Denmark Technical University, 2800 Lyngby, Denmark
3
Safety and Environmental Assurance Centre, Colworth Science Park, Unilever plc, Sharnbrook, Bedford MK44 1LQ, UK
4
School of Chemical Engineering and Analytical Science, University of Manchester, Oxford Road, Manchester M13 9PL, UK
*
Authors to whom correspondence should be addressed.
Received: 21 January 2018 / Revised: 14 February 2018 / Accepted: 20 March 2018 / Published: 27 March 2018
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Abstract

A sensitive and label-free surface plasmon resonance (SPR) based sensor was developed in this work for the detection of milk allergens. β-lactoglobulin (BLG) protein was used as the biomarker for cow milk detection. This is to be used directly in final rinse samples of cleaning in-place (CIP) systems of food manufacturers. The affinity assay was optimised and characterised before a standard curve was performed in pure buffer conditions, giving a detection limit of 0.164 µg mL−1 as a direct binding assay. The detection limit can be further enhanced through the use of a sandwich assay and amplification with nanomaterials. However, this was not required here, as the detection limit achieved exceeded the required allergen detection levels of 2 µg mL−1 for β-lactoglobulin. The binding affinities of the polyclonal antibody for BLG, expressed by the dissociation constant (KD), were equal to 2.59 × 10−9 M. The developed SPR-based sensor offers several advantages in terms of label-free detection, real-time measurements, potential on-line system and superior sensitivity when compared to ELISA-based techniques. The method is novel for this application and could be applied to wider food allergen risk management decision(s) in food manufacturing. View Full-Text
Keywords: allergen; milk protein; β-lactoglobulin (BLG); surface plasmon resonance (SPR); biosensor allergen; milk protein; β-lactoglobulin (BLG); surface plasmon resonance (SPR); biosensor
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Ashley, J.; D’Aurelio, R.; Piekarska, M.; Temblay, J.; Pleasants, M.; Trinh, L.; Rodgers, T.L.; Tothill, I.E. Development of a β-Lactoglobulin Sensor Based on SPR for Milk Allergens Detection. Biosensors 2018, 8, 32.

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