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Biosensors 2018, 8(3), 77; https://doi.org/10.3390/bios8030077

Development of MoSe2 Nano-Urchins as a Sensing Platform for a Selective Bio-Capturing of Escherichia coli Shiga Toxin DNA

1
Amity Institute of Nanotechnology, Amity University, Noida 201313, India
2
CNR-IC, Area della Ricercadi RM1, Via Salaria km 29.3, Monterotondo, I-00015 Rome, Italy
3
Department of Biochemistry, MD University, Rohtak 124001, India
4
Centre for Nanoscience and Nanotechnology, Jamia Millia Islamia University, New Delhi 110025, India
*
Authors to whom correspondence should be addressed.
Received: 14 June 2018 / Revised: 9 August 2018 / Accepted: 10 August 2018 / Published: 14 August 2018
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Abstract

The present study was aimed to develop “fluorine doped” tin oxide glass electrode with a MoSe2 nano-urchin based electrochemical biosensor for detection of Escherichia coli Shiga toxin DNA. The study comprises two conductive electrodes, and the working electrodes were drop deposited using MoSe2 nano-urchin, and DNA sequences specific to Shiga toxin Escherichia coli. Morphological characterizations were performed using Fourier transforms infrared spectrophotometer; X-ray diffraction technique and scanning electron microscopy. All measurements were done using methylene blue as an electrochemical indicator. The proposed electrochemical geno-sensor showed good linear detection range of 1 fM–100 µM with a low detection limit of 1 fM where the current response increased linearly with Escherichia coli Shiga toxin dsDNA concentration with R2 = 0.99. Additionally, the real sample was spiked with the dsDNA that shows insignificant interference. The results revealed that the developed sensing platform significantly improved the sensitivity and can provide a promising platform for effective detection of biomolecules using minute samples due to its stability and sensitivity. View Full-Text
Keywords: fluorine doped tin oxide (FTO); molybdenum diselenide nano-urchin; geno-sensor; methylene blue (MB) fluorine doped tin oxide (FTO); molybdenum diselenide nano-urchin; geno-sensor; methylene blue (MB)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Narang, J.; Mishra, A.; Pilloton, R.; VV, A.; Wadhwa, S.; Pundir, C.S.; Khanuja, M. Development of MoSe2 Nano-Urchins as a Sensing Platform for a Selective Bio-Capturing of Escherichia coli Shiga Toxin DNA. Biosensors 2018, 8, 77.

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