Interleukin-10 Conjugation to Carboxylated PVP-Coated Silver Nanoparticles for Improved Stability and Therapeutic Efficacy
Abstract
:1. Introduction
2. Results
2.1. Activation of PVP with Carboxylic Acid Groups
2.2. IL-10 Conjugation to Carboxylated PVP-Coated Silver Nanoparticles
2.3. Cytotoxicity Effect of rmIL-10 Conjugated Ag-PVPs
2.4. rmIL-10 Conjugated Ag-PVPs Reduced LPS-Induced Inflammatory Responses
3. Discussion
4. Materials and Methods
4.1. Carboxylation of PVP
4.2. Synthesis of Carboxylated PVP-Coated Silver Nanoparticles
4.3. IL-10 Conjugation and Characterization of Nanoparticles
4.4. Cell Culture
4.5. Cytotoxicity Study
4.6. Cell Activation and Analysis of IL-10 Activity
4.7. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Baganizi, D.R.; Nyairo, E.; Duncan, S.A.; Singh, S.R.; Dennis, V.A. Interleukin-10 Conjugation to Carboxylated PVP-Coated Silver Nanoparticles for Improved Stability and Therapeutic Efficacy. Nanomaterials 2017, 7, 165. https://doi.org/10.3390/nano7070165
Baganizi DR, Nyairo E, Duncan SA, Singh SR, Dennis VA. Interleukin-10 Conjugation to Carboxylated PVP-Coated Silver Nanoparticles for Improved Stability and Therapeutic Efficacy. Nanomaterials. 2017; 7(7):165. https://doi.org/10.3390/nano7070165
Chicago/Turabian StyleBaganizi, Dieudonné R., Elijah Nyairo, Skyla A. Duncan, Shree R. Singh, and Vida A. Dennis. 2017. "Interleukin-10 Conjugation to Carboxylated PVP-Coated Silver Nanoparticles for Improved Stability and Therapeutic Efficacy" Nanomaterials 7, no. 7: 165. https://doi.org/10.3390/nano7070165