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Nanomaterials 2017, 7(2), 30; doi:10.3390/nano7020030

Magnetic Nanovectors for the Development of DNA Blood-Stage Malaria Vaccines

1
Department of Chemical Engineering, Monash University, 18 Alliance Lane, Clayton, VIC 3800, Australia
2
Department of Immunology and Pathology, Central Clinical School, Faculty of Medicine, Nursing and Health Sciences, Monash University, 89 Commercial Road, Melbourne, VIC 3004, Australia
3
Department of Microbiology, Monash University, Wellington Road, Clayton, VIC 3800, Australia
Current Address: Department of Animal resources, College of Agriculture, Kirkuk University, Kirkuk, Iraq
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Neil OBrien-Simpson
Received: 1 November 2016 / Revised: 18 January 2017 / Accepted: 25 January 2017 / Published: 10 February 2017
(This article belongs to the Special Issue Nanoparticles in Immunology)
View Full-Text   |   Download PDF [960 KB, uploaded 13 February 2017]   |  

Abstract

DNA vaccines offer cost, flexibility, and stability advantages, but administered alone have limited immunogenicity. Previously, we identified optimal configurations of magnetic vectors comprising superparamagnetic iron oxide nanoparticles (SPIONs), polyethylenimine (PEI), and hyaluronic acid (HA) to deliver malaria DNA encoding Plasmodium yoelii (Py) merozoite surface protein MSP119 (SPIONs/PEI/DNA + HA gene complex) to dendritic cells and transfect them with high efficiency in vitro. Herein, we evaluate their immunogenicity in vivo by administering these potential vaccine complexes into BALB/c mice. The complexes induced antibodies against PyMSP119, with higher responses induced intraperitoneally than intramuscularly, and antibody levels further enhanced by applying an external magnetic field. The predominant IgG subclasses induced were IgG2a followed by IgG1 and IgG2b. The complexes further elicited high levels of interferon gamma (IFN-γ), and moderate levels of interleukin (IL)-4 and IL-17 antigen-specific splenocytes, indicating induction of T helper 1 (Th1), Th2, and Th17 cell mediated immunity. The ability of such DNA/nanoparticle complexes to induce cytophilic antibodies together with broad spectrum cellular immunity may benefit malaria vaccines. View Full-Text
Keywords: hyaluronic acid; MSP119; superparamagnetic iron oxide nanoparticles (SPIONs); magnetic gene vector; malaria DNA vaccine; antibody; immune response hyaluronic acid; MSP119; superparamagnetic iron oxide nanoparticles (SPIONs); magnetic gene vector; malaria DNA vaccine; antibody; immune response
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Al-Deen, F.M.N.; Xiang, S.D.; Ma, C.; Wilson, K.; Coppel, R.L.; Selomulya, C.; Plebanski, M. Magnetic Nanovectors for the Development of DNA Blood-Stage Malaria Vaccines. Nanomaterials 2017, 7, 30.

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