Next Article in Journal
Stress Waves and Characteristics of Zigzag and Armchair Silicene Nanoribbons
Next Article in Special Issue
Nanomaterials for Cardiac Myocyte Tissue Engineering
Previous Article in Journal
Nanoparticles for Catalysis
Previous Article in Special Issue
The Influence of Modified Silica Nanomaterials on Adult Stem Cell Culture
Article Menu

Export Article

Open AccessArticle
Nanomaterials 2016, 6(7), 124; doi:10.3390/nano6070124

Nanostructured TiO2 Surfaces Promote Human Bone Marrow Mesenchymal Stem Cells Differentiation to Osteoblasts

1
Department of Molecular Medicine, Center for Health Technologies (CHT), UdR INSTM, University of Pavia, Viale Taramelli 3/b, Pavia 27100, Italy
2
Department of Public Health, Experimental Medicine and Forensic, Human Anatomy Unit, Center of Health Technologies (CHT), University of Pavia, Viale Forlanini 8, Pavia 27100, Italy
3
Department of Chemistry-Physical-Chemistry Section, University of Pavia, Viale Taramelli 16, Pavia 27100, Italy
4
Laboratory of Transplant Immunology/Cell Factory, Fondazione IRCCS Policlinico “San Matteo”, P.le Golgi 19, Pavia 27100, Italy
5
Department of Occupational Medicine, Toxicology and Environmental Risks, S. Maugeri Foundation, IRCCS, Via S.Boezio 28, Pavia 27100, Italy
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Ilaria Armentano
Received: 11 May 2016 / Revised: 13 June 2016 / Accepted: 15 June 2016 / Published: 24 June 2016
(This article belongs to the Special Issue Nanomaterials for Tissue Engineering)
View Full-Text   |   Download PDF [3267 KB, uploaded 24 June 2016]   |  

Abstract

Micro- and nano-patterning/modification are emerging strategies to improve surfaces properties that may influence critically cells adherence and differentiation. Aim of this work was to study the in vitro biological reactivity of human bone marrow mesenchymal stem cells (hBMSCs) to a nanostructured titanium dioxide (TiO2) surface in comparison to a coverglass (Glass) in two different culture conditions: with (osteogenic medium (OM)) and without (proliferative medium (PM)) osteogenic factors. To evaluate cell adhesion, hBMSCs phosphorylated focal adhesion kinase (pFAK) foci were analyzed by confocal laser scanning microscopy (CLSM) at 24 h: the TiO2 surface showed a higher number of pFAK foci with respect to Glass. The hBMSCs differentiation to osteoblasts was evaluated in both PM and OM culture conditions by enzyme-linked immunosorbent assay (ELISA), CLSM and real-time quantitative reverse transcription PCR (qRT-PCR) at 28 days. In comparison with Glass, TiO2 surface in combination with OM conditions increased the content of extracellular bone proteins, calcium deposition and alkaline phosphatase activity. The qRT-PCR analysis revealed, both in PM and OM, that TiO2 surface increased at seven and 28 days the expression of osteogenic genes. All together, these results demonstrate the capability of TiO2 nanostructured surface to promote hBMSCs osteoblast differentiation and its potentiality in biomedical applications. View Full-Text
Keywords: human bone marrow mesenchymal stem cells; bone tissue engineering and regeneration; nano-patterning modification; titanium dioxide surface; biomedical applications human bone marrow mesenchymal stem cells; bone tissue engineering and regeneration; nano-patterning modification; titanium dioxide surface; biomedical applications
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Vercellino, M.; Ceccarelli, G.; Cristofaro, F.; Balli, M.; Bertoglio, F.; Bruni, G.; Benedetti, L.; Avanzini, M.A.; Imbriani, M.; Visai, L. Nanostructured TiO2 Surfaces Promote Human Bone Marrow Mesenchymal Stem Cells Differentiation to Osteoblasts. Nanomaterials 2016, 6, 124.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Nanomaterials EISSN 2079-4991 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top