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Nanomaterials 2016, 6(6), 104; doi:10.3390/nano6060104

The Influence of Modified Silica Nanomaterials on Adult Stem Cell Culture

1
Department of Chemistry, Biology and Biotechnology, University of Perugia, Via Elce di Sotto, 8, 06123 Perugia, Italy
2
Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123 Perugia, Italy
*
Author to whom correspondence should be addressed.
Academic Editors: Ilaria Armentano, Luigi Torre and Josè Maria Kenny
Received: 2 May 2016 / Revised: 23 May 2016 / Accepted: 24 May 2016 / Published: 4 June 2016
(This article belongs to the Special Issue Nanomaterials for Tissue Engineering)
View Full-Text   |   Download PDF [1751 KB, uploaded 4 June 2016]   |  

Abstract

The preparation of tailored nanomaterials able to support cell growth and viability is mandatory for tissue engineering applications. In the present work, silica nanoparticles were prepared by a sol-gel procedure and were then functionalized by condensation of amino groups and by adsorption of silver nanoparticles. Transmission electron microscopy (TEM) imaging was used to establish the morphology and the average dimensions of about 130 nm, which were not affected by the functionalization. The three silica samples were deposited (1 mg/mL) on cover glasses, which were used as a substrate to culture adult human bone marrow-mesenchymal stem cells (hBM-MSCs) and human adipose-derived stem cells (hASCs). The good cell viability over the different silica surfaces was evaluated by monitoring the mitochondrial dehydrogenase activity. The analysis of the morphological parameters (aspect ratio, cell length, and nuclear shape Index) yielded information about the interactions of stem cells with the surface of three different nanoparticles. The data are discussed in terms of chemical properties of the surface of silica nanoparticles. View Full-Text
Keywords: silica nanoparticles; surface functionalization; adult stem cell; stem cell viability; cell morphology; fluorescence imaging silica nanoparticles; surface functionalization; adult stem cell; stem cell viability; cell morphology; fluorescence imaging
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Tarpani, L.; Morena, F.; Gambucci, M.; Zampini, G.; Massaro, G.; Argentati, C.; Emiliani, C.; Martino, S.; Latterini, L. The Influence of Modified Silica Nanomaterials on Adult Stem Cell Culture. Nanomaterials 2016, 6, 104.

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