Nanomaterials 2014, 4(2), 522-534; doi:10.3390/nano4020522
Article

Reducing X-Ray Induced Oxidative Damages in Fibroblasts with Graphene Oxide

Received: 1 April 2014; in revised form: 17 June 2014 / Accepted: 19 June 2014 / Published: 24 June 2014
(This article belongs to the Special Issue Nanotoxicology)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: A major issue of X-ray radiation therapy is that normal cells can be damaged, limiting the amount of X-rays that can be safely delivered to a tumor. This paper describes a new method based on graphene oxide (GO) to protect normal cells from oxidative damage by removing free radicals generated by X-ray radiation using grapheme oxide (GO). A variety of techniques such as cytotoxicity, genotoxicity, oxidative assay, apoptosis, γ-H2AX expression, and micro-nucleus assay have been used to assess the protective effect of GO in cultured fibroblast cells. It is found that although GO at higher concentration (100 and 500 µg/mL) can cause cell death and DNA damage, it can effectively remove oxygen free radicals at a lower concentration of 10 µg/mL. The level of DNA damage and cell death is reduced by 48%, and 39%, respectively. Thus, low concentration GO can be used as an effective radio-protective agent in occupational and therapeutic settings.
Keywords: X-ray; graphene oxide; DNA damage; oxidative; reactive oxygen species (ROS)
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MDPI and ACS Style

Qiao, Y.; Zhang, P.; Wang, C.; Ma, L.; Su, M. Reducing X-Ray Induced Oxidative Damages in Fibroblasts with Graphene Oxide. Nanomaterials 2014, 4, 522-534.

AMA Style

Qiao Y, Zhang P, Wang C, Ma L, Su M. Reducing X-Ray Induced Oxidative Damages in Fibroblasts with Graphene Oxide. Nanomaterials. 2014; 4(2):522-534.

Chicago/Turabian Style

Qiao, Yong; Zhang, Peipei; Wang, Chaoming; Ma, Liyuan; Su, Ming. 2014. "Reducing X-Ray Induced Oxidative Damages in Fibroblasts with Graphene Oxide." Nanomaterials 4, no. 2: 522-534.

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