Nanomaterials 2012, 2(2), 187-205; doi:10.3390/nano2020187
Article

Amino Acid and Peptide Immobilization on Oxidized Nanocellulose: Spectroscopic Characterization

* email and email
Received: 18 April 2012; in revised form: 24 May 2012 / Accepted: 5 June 2012 / Published: 12 June 2012
(This article belongs to the Special Issue Composite Nanomaterials)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: In this work, oxidized nanocellulose (ONC) was synthesized and chemically coupled with amino acids and peptides using a two step coupling method at room temperature. First, ONC was activated by N-ethyl-N’-(3-dimethylaminopropyl) carbodiimide hydrochloride, forming a stable active ester in the presence of N-hydroxysuccinimide. Second, the active ester was reacted with the amino group of the amino acid or peptide, forming an amide bond between ONC and the grafted molecule. Using this method, the intermolecular interaction of amino acids and peptides was avoided and uniform coupling of these molecules on ONC was achieved. The coupling reaction was very fast in mild conditions and without alteration of the polysaccharide. The coupling products (ONC-amino acids and ONC-peptides) were characterized by transmission electron microscopy and by the absorption, emission, Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) spectroscopic techniques.
Keywords: oxidized nanocellulose; amino acids; peptides; amide bond; spectroscopic characterization
PDF Full-text Download PDF Full-Text [421 KB, uploaded 12 June 2012 08:33 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Barazzouk, S.; Daneault, C. Amino Acid and Peptide Immobilization on Oxidized Nanocellulose: Spectroscopic Characterization. Nanomaterials 2012, 2, 187-205.

AMA Style

Barazzouk S, Daneault C. Amino Acid and Peptide Immobilization on Oxidized Nanocellulose: Spectroscopic Characterization. Nanomaterials. 2012; 2(2):187-205.

Chicago/Turabian Style

Barazzouk, Saïd; Daneault, Claude. 2012. "Amino Acid and Peptide Immobilization on Oxidized Nanocellulose: Spectroscopic Characterization." Nanomaterials 2, no. 2: 187-205.

Nanomaterials EISSN 2079-4991 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert