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Amino Acid and Peptide Immobilization on Oxidized Nanocellulose: Spectroscopic Characterization
Centre de Recherche sur les Matériaux Lignocellulosiques, Université du Québec à Trois-Rivières, Trois-Rivières, QC, G9A 5H7, Canada
* Author to whom correspondence should be addressed.
Received: 18 April 2012; in revised form: 24 May 2012 / Accepted: 5 June 2012 / Published: 12 June 2012
Abstract: In this work, oxidized nanocellulose (ONC) was synthesized and chemically coupled with amino acids and peptides using a two step coupling method at room temperature. First, ONC was activated by N-ethyl-N’-(3-dimethylaminopropyl) carbodiimide hydrochloride, forming a stable active ester in the presence of N-hydroxysuccinimide. Second, the active ester was reacted with the amino group of the amino acid or peptide, forming an amide bond between ONC and the grafted molecule. Using this method, the intermolecular interaction of amino acids and peptides was avoided and uniform coupling of these molecules on ONC was achieved. The coupling reaction was very fast in mild conditions and without alteration of the polysaccharide. The coupling products (ONC-amino acids and ONC-peptides) were characterized by transmission electron microscopy and by the absorption, emission, Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) spectroscopic techniques.
Keywords: oxidized nanocellulose; amino acids; peptides; amide bond; spectroscopic characterization
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MDPI and ACS Style
Barazzouk, S.; Daneault, C. Amino Acid and Peptide Immobilization on Oxidized Nanocellulose: Spectroscopic Characterization. Nanomaterials 2012, 2, 187-205.
Barazzouk S, Daneault C. Amino Acid and Peptide Immobilization on Oxidized Nanocellulose: Spectroscopic Characterization. Nanomaterials. 2012; 2(2):187-205.
Barazzouk, Saïd; Daneault, Claude. 2012. "Amino Acid and Peptide Immobilization on Oxidized Nanocellulose: Spectroscopic Characterization." Nanomaterials 2, no. 2: 187-205.