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A correction was published on 17 March 2014, see J. Funct. Biomater. 2014, 5(1), 27-28.

J. Funct. Biomater. 2013, 4(3), 162-177; doi:10.3390/jfb4030162
Article

Epoxy Cross-Linked Collagen and Collagen-Laminin Peptide Hydrogels as Corneal Substitutes

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Received: 7 April 2013; in revised form: 3 July 2013 / Accepted: 6 August 2013 / Published: 28 August 2013
(This article belongs to the Special Issue Advances in Ophthalmic Biomaterials)
View Full-Text   |   Download PDF [574 KB, updated 2 September 2013; original version uploaded 28 August 2013]
Abstract: A bi-functional epoxy-based cross-linker, 1,4-Butanediol diglycidyl ether (BDDGE), was investigated in the fabrication of collagen based corneal substitutes. Two synthetic strategies were explored in the preparation of the cross-linked collagen scaffolds. The lysine residues of Type 1 porcine collagen were directly cross-linked using l,4-Butanediol diglycidyl ether (BDDGE) under basic conditions at pH 11. Alternatively, under conventional methodology, using both BDDGE and 1-Ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) as cross-linkers, hydrogels were fabricated under acidic conditions. In this latter strategy, Cu(BF4)2·XH2O was used to catalyze the formation of secondary amine bonds. To date, we have demonstrated that both methods of chemical cross-linking improved the elasticity and tensile strength of the collagen implants. Differential scanning calorimetry and biocompatibility studies indicate comparable, and in some cases, enhanced properties compared to that of the EDC/NHS controls. In vitro studies showed that human corneal epithelial cells and neuronal progenitor cell lines proliferated on these hydrogels. In addition, improvement of cell proliferation on the surfaces of the materials was observed when neurite promoting laminin epitope, IKVAV, and adhesion peptide, YIGSR, were incorporated. However, the elasticity decreased with peptide incorporation and will require further optimization. Nevertheless, we have shown that epoxy cross-linkers should be further explored in the fabrication of collagen-based hydrogels, as alternatives to or in conjunction with carbodiimide cross-linkers.
Keywords: biomimetic materials; cross-linking; collagen; cornea; tissue engineering biomimetic materials; cross-linking; collagen; cornea; tissue engineering
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • Correction

    Correction (PDF, 201 KB)

    A correction was published on 17 March 2014 (http://www.mdpi.com/2079-4983/5/1/27)

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MDPI and ACS Style

Koh, L.B.; Islam, M.M.; Mitra, D.; Noel, C.W.; Merrett, K.; Odorcic, S.; Fagerholm, P.; Jackson, W.B.; Liedberg, B.; Phopase, J.; Griffith, M. Epoxy Cross-Linked Collagen and Collagen-Laminin Peptide Hydrogels as Corneal Substitutes. J. Funct. Biomater. 2013, 4, 162-177.

AMA Style

Koh LB, Islam MM, Mitra D, Noel CW, Merrett K, Odorcic S, Fagerholm P, Jackson WB, Liedberg B, Phopase J, Griffith M. Epoxy Cross-Linked Collagen and Collagen-Laminin Peptide Hydrogels as Corneal Substitutes. Journal of Functional Biomaterials. 2013; 4(3):162-177.

Chicago/Turabian Style

Koh, Li B.; Islam, Mohammad M.; Mitra, Debbie; Noel, Christopher W.; Merrett, Kimberley; Odorcic, Silvia; Fagerholm, Per; Jackson, William. B.; Liedberg, Bo; Phopase, Jaywant; Griffith, May. 2013. "Epoxy Cross-Linked Collagen and Collagen-Laminin Peptide Hydrogels as Corneal Substitutes." J. Funct. Biomater. 4, no. 3: 162-177.



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