Next Article in Journal
Potentials of Chitosan-Based Delivery Systems in Wound Therapy: Bioadhesion Study
Next Article in Special Issue
Finite-Element Modeling of Viscoelastic Cells During High-Frequency Cyclic Strain
Previous Article in Journal
A Path to Soluble Molecularly Imprinted Polymers
J. Funct. Biomater. 2012, 3(1), 23-36; doi:10.3390/jfb3010023
Article

Biomechanical Conditioning Enhanced Matrix Synthesis in Nucleus Pulposus Cells Cultured in Agarose Constructs with TGFβ

1
, 1,2
 and 1,*
Received: 30 November 2011; in revised form: 23 December 2011 / Accepted: 28 December 2011 / Published: 5 January 2012
(This article belongs to the Special Issue Mechanics of Cells in Context with Biomaterials)
View Full-Text   |   Download PDF [368 KB, uploaded 5 January 2012]   |   Browse Figures
Abstract: Biomechanical signals play an important role in normal disc metabolism and pathology. For instance, nucleus pulposus (NP) cells will regulate metabolic activities and maintain a balance between the anabolic and catabolic cascades. The former involves factors such as transforming growth factor-β (TGFβ) and mechanical stimuli, both of which are known to regulate matrix production through autocrine and paracrine mechanisms. The present study examined the combined effect of TGFβ and mechanical loading on anabolic activities in NP cells cultured in agarose constructs. Stimulation with TGFβ and dynamic compression reduced nitrite release and increased matrix synthesis and gene expression of aggrecan and collagen type II. The findings from this work has the potential for developing regenerative treatment strategies which could either slow down or stop the degenerative process and/or promote healing mechanisms in the intervertebral disc.
Keywords: TGFβ; mechanical loading; matrix synthesis; nucleus pulposus; intervertebral disc TGFβ; mechanical loading; matrix synthesis; nucleus pulposus; intervertebral disc
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Tilwani, R.K.; Bader, D.L.; Chowdhury, T.T. Biomechanical Conditioning Enhanced Matrix Synthesis in Nucleus Pulposus Cells Cultured in Agarose Constructs with TGFβ. J. Funct. Biomater. 2012, 3, 23-36.

AMA Style

Tilwani RK, Bader DL, Chowdhury TT. Biomechanical Conditioning Enhanced Matrix Synthesis in Nucleus Pulposus Cells Cultured in Agarose Constructs with TGFβ. Journal of Functional Biomaterials. 2012; 3(1):23-36.

Chicago/Turabian Style

Tilwani, Reshma K.; Bader, Dan L.; Chowdhury, Tina T. 2012. "Biomechanical Conditioning Enhanced Matrix Synthesis in Nucleus Pulposus Cells Cultured in Agarose Constructs with TGFβ." J. Funct. Biomater. 3, no. 1: 23-36.


J. Funct. Biomater. EISSN 2079-4983 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert