J. Funct. Biomater. 2011, 2(3), 213-229; doi:10.3390/jfb2030213
Article

Self-Assembled Matrix by Umbilical Cord Stem Cells

1email, 2email, 1email, 2email, 3email, 2email and 1,* email
Received: 3 August 2011; in revised form: 23 August 2011 / Accepted: 25 August 2011 / Published: 1 September 2011
(This article belongs to the Special Issue Biomaterials for Wound Healing)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Corneal integrity is critical for vision. Corneal wounds frequently heal with scarring that impairs vision. Recently, human umbilical cord mesenchymal stem cells (cord stem cells) have been investigated for tissue engineering and therapy due to their availability and differentiation potential. In this study, we used cord stem cells in a 3-dimensional (3D) stroma-like model to observe extracellular matrix organization, with human corneal fibroblasts acting as a control. For 4 weeks, the cells were stimulated with a stable Vitamin C (VitC) derivative ±TGF-b1. After 4 weeks, the mean thickness of the constructs was ~30 mm; however, cord stem cell constructs had 50% less cells per unit volume, indicating the formation of a dense matrix. We found minimal change in decorin and lumican mRNA, and a significant increase in perlecan mRNA in the presence of TGF-b1. Keratocan on the other hand decreased with TGF-b1 in both cell lineages. With both cell types, the constructs possessed aligned collagen fibrils and associated glycosaminoglycans. Fibril diameters did not change with TGF-b1 stimulation or cell lineage; however, highly sulfated glycosaminoglycans associated with the collagen fibrils significantly increased with TGF-b1. Overall, we have shown that cord stem cells can secrete their own extracellular matrix and promote the deposition and sulfation of various proteoglycans. Furthermore, these cells are at least comparable to commonly used corneal fibroblasts and present an alternative for the 3D in vitro tissue engineered model.
Keywords: stem cells; extracellular matrix; cornea; glycosaminoglycans
PDF Full-text Download PDF Full-Text [1002 KB, Updated Version, uploaded 2 September 2011 10:17 CEST]
The original version is still available [1002 KB, uploaded 1 September 2011 14:26 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Karamichos, D.; Rich, C.B.; Hutcheon, A.E.; Ren, R.; Saitta, B.; Trinkaus-Randall, V.; Zieske, J.D. Self-Assembled Matrix by Umbilical Cord Stem Cells. J. Funct. Biomater. 2011, 2, 213-229.

AMA Style

Karamichos D, Rich CB, Hutcheon AE, Ren R, Saitta B, Trinkaus-Randall V, Zieske JD. Self-Assembled Matrix by Umbilical Cord Stem Cells. Journal of Functional Biomaterials. 2011; 2(3):213-229.

Chicago/Turabian Style

Karamichos, Dimitrios; Rich, Celeste B.; Hutcheon, Audrey E.K.; Ren, Ruiyi; Saitta, Biagio; Trinkaus-Randall, Vickery; Zieske, James D. 2011. "Self-Assembled Matrix by Umbilical Cord Stem Cells." J. Funct. Biomater. 2, no. 3: 213-229.

J. Funct. Biomater. EISSN 2079-4983 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert