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Vaccines 2016, 4(2), 15; doi:10.3390/vaccines4020015

Coupling Peptide Antigens to Virus-Like Particles or to Protein Carriers Influences the Th1/Th2 Polarity of the Resulting Immune Response

1
Department of Microbiology, School of Medicine, Prince of Songkla University, Hadyai, Songkla 90110, Thailand
2
Faculty of Allied Health Sciences, Burapha University, Bangsaen, Chonburi 20131, Thailand
3
Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, USA
4
Department of Plant Sciences, Montana State University, Bozeman, MT 59717, USA
5
Departments of Pediatrics and Microbiology, School of Medicine, Louisianna State University, New Orleans, LA 70118, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Katherine A. Wall
Received: 25 February 2016 / Revised: 22 April 2016 / Accepted: 26 April 2016 / Published: 5 May 2016
(This article belongs to the Special Issue Glycopeptide-based and Related Vaccines)
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Abstract

We have conjugated the S9 peptide, a mimic of the group B streptococcal type III capsular polysaccharide, to different carriers in an effort to elicit an optimal immune response. As carriers, we utilized the soluble protein keyhole limpet hemocyanin and virus-like particles (VLPs) from two plant viruses, Cowpea Chlorotic Mottle Virus and Cowpea Mosaic Virus. We have found that coupling the peptide to the soluble protein elicits a Th2 immune response, as evidenced by the production of the peptide-specific IgG1 antibody and IL-4/IL-10 production in response to antigen stimulation, whereas the peptide conjugated to VLPs elicited a Th1 response (IgG2a, IFN-γ). Because the VLPs used as carriers package RNA during the assembly process, we hypothesize that this effect may result from the presence of nucleic acid in the immunogen, which affects the Th1/Th2 polarity of the response. View Full-Text
Keywords: group B streptococcus; Streptococcus agalactiae; capsular polysaccharide; antigen; mimotope; synthetic peptide group B streptococcus; Streptococcus agalactiae; capsular polysaccharide; antigen; mimotope; synthetic peptide
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Pomwised, R.; Intamaso, U.; Teintze, M.; Young, M.; Pincus, S.H. Coupling Peptide Antigens to Virus-Like Particles or to Protein Carriers Influences the Th1/Th2 Polarity of the Resulting Immune Response. Vaccines 2016, 4, 15.

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