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Vaccines 2016, 4(1), 7; doi:10.3390/vaccines4010007

Intranasal Vaccination Affords Localization and Persistence of Antigen-Specific CD8+ T Lymphocytes in the Female Reproductive Tract

1
Department of Immunology, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77054, USA
2
Immunology Graduate Program, The University of Texas Graduate School of Biomedical Sciences at Houston, Houston, TX 77030, USA
3
Department of Internal Medicine, Mayo Clinic, Rochester, MN 55905, USA
4
Department of Molecular Medicine, Mayo Clinic, Rochester, MN 55905, USA
5
Division of Infectious Diseases, Mayo Clinic, Rochester, MN 55905, USA
6
Translational Immunovirology Program, Mayo Clinic, Rochester, MN 55905, USA
7
Department of Immunology, Mayo Clinic, Rochester, MN 55905, USA
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Diane M. Harper
Received: 21 January 2016 / Revised: 4 March 2016 / Accepted: 11 March 2016 / Published: 17 March 2016
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Abstract

Immunization strategies generating large numbers of antigen-specific T cells in the female reproductive tract (FRT) can provide barrier protection against sexually-transmitted pathogens, such as the human immunodeficiency virus (HIV) and human papillomaviruses (HPV). The kinetics and mechanisms of regulation of vaccine-induced adaptive T cell-mediated immune responses in FRT are less well defined. We present here evidence for intranasal delivery of the model antigen ovalbumin (OVA) along with alpha-galactosylceramide adjuvant as a protein vaccine to induce significantly higher levels of antigen-specific effector and memory CD8+ T cells in the FRT, relative to other systemic and mucosal tissues. Antibody blocking of the CXCR3 receptor significantly reduced antigen-specific CD8+ T cells subsequent to intranasal delivery of the protein vaccine suggesting an important role for the CXCR3 chemokine-receptor signaling for T cell trafficking. Further, intranasal vaccination with an adenoviral vector expressing OVA or HIV-1 envelope was as effective as intramuscular vaccination for generating OVA- or ENV-specific immunity in the FRT. These results support the application of the needle-free intranasal route as a practical approach to delivering protein as well as DNA/virus vector-based vaccines for efficient induction of effector and memory T cell immunity in the FRT. View Full-Text
Keywords: Female reproductive tract; CD8+ T cells; intranasal immunization Female reproductive tract; CD8+ T cells; intranasal immunization
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Singh, S.; Schluns, K.S.; Yang, G.; Anthony, S.M.; Barry, M.A.; Sastry, K.J. Intranasal Vaccination Affords Localization and Persistence of Antigen-Specific CD8+ T Lymphocytes in the Female Reproductive Tract. Vaccines 2016, 4, 7.

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