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Antioxidants 2013, 2(4), 340-369; doi:10.3390/antiox2040340
Published: 26 November 2013
Abstract: This short review briefly introduces the formation of reactive oxygen species (ROS) as by-products of oxidation/reduction (redox) reactions, and the ways in which the antioxidant defense machinery is involved directly or indirectly in ROS scavenging. Major antioxidants, both enzymatic and non enzymatic, that protect higher plant cells from oxidative stress damage are described. Biochemical and molecular features of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) are discussed because they play crucial roles in scavenging ROS in the different cell compartments and in response to stress conditions. Among the non enzymatic defenses, particular attention is paid to ascorbic acid, glutathione, flavonoids, carotenoids, and tocopherols. The operation of ROS scavenging systems during the seasonal cycle and specific developmental events, such as fruit ripening and senescence, are discussed in relation to the intense ROS formation during these processes that impact fruit quality. Particular attention is paid to Prunus and Citrus species because of the nutritional and antioxidant properties contained in these commonly consumed fruits.
Antioxidants are compounds produced by aerobic organisms to counteract oxidative stress caused by an imbalance of reactive oxygen species (ROS). ROS include the superoxide anion radicals and the hydroxyl radicals produced as by-products of oxidation/reduction (redox) reactions as a consequence of aerobic metabolism .
At the very beginning, the earth’s atmosphere was essentially free of oxygen. Presumably, the most primitive organisms were anaerobic heterotrophs, living protected from solar ionizing radiation damage in the ocean abysses. The earliest, relatively low levels of oxygen probably resulted from photolytic dissociation of water caused by the sun’s ionizing radiation. Later, photosynthetic activities of cyanobacteria and plants steadily boosted oxygen levels, and aerobic metabolism evolved. As a consequence of increasing oxygen levels, all organisms exposed to an aerobic environment are subjected to the toxic effects of ROS.
Molecular oxygen is relatively non-reactive in its normal configuration. During normal metabolic activity and as a consequence of environmental variations, O2 can generate reactive excited states such as free radicals and derivatives: the singlet oxygen (1O2), superoxide radical (O2•−), hydrogen peroxide (H2O2), and the hydroxyl radical (OH•). The latter is the most potent oxidant known. ROS attack biomolecules causing DNA mutation, protein denaturation and membrane lipid peroxidation; these oxidations disturb normal cellular metabolism and cause molecular damage, and if sufficiently severe can result in cell death.
In plants, the two major sources of ROS are the photosynthetic and respiratory electron transport chains. Chloroplasts and peroxisomes are the main source of ROS in the light, while in darkness the mitochondria seem to be the main ROS producers. ROS are detected in virtually all intracellular organelles, as well as at the plasma membrane and, extra-cellularly, in the apoplast.
Environmental stresses including intense light, UV, temperature stress, heat/cold treatments, drought, and herbicides have all been demonstrated to increase ROS production [2,3]. In acute or prolonged treatments, the ROS generated can overwhelm the constitutive systems necessitating additional defenses. Thus, ironically, oxygen that it is essential for aerobic life—in its reduced forms—is one of the most toxic substances with which all organisms must cope. During the course of evolution, plants gained a high degree of control over ROS toxicity and are utilizing ROS as signal molecules for initiating defense mechanisms to sustain ROS homeostasis. Moreover, ROS play crucial roles in developmental processes in all living organisms . These molecules are also important in plant responses to pathogens and immune defenses in mammals . Controlling ROS toxicity, while modulating ROS as signals, requires a large gene network that in Arabidopsis is composed by at least 152 genes .
Regulation of multiple redox reactions and ROS signals in plants requires a high degree of coordination and balance between signaling and metabolic pathways during a stress response in which cellular redox homeostasis of the cell is disrupted. This disturbance in equilibrium leads to sudden increase of intracellular level of ROS, and it has been estimated that 1%–2% of O2 consumed by plants is diverted to produce ROS in various subcellular loci. Given the challenge imposed by plant oxygen-evolving capability, each organelle or compartment has evolved mechanisms for the elimination of excess ROS accumulation (Figure 1).
ROS are not the only reactive molecules generated as by-products of enzymatic reactions. Recently, great attention has been paid to reactive nitrogen species (RNS) produced by a group of enzymes called nitric oxide (NO) synthases. As with ROS, the RNS cause biological damages because of their reactivity. Here, I will focus on ROS only.
2. Defense against ROS
ROS homeostasis in cells is reached at the balance between ROS production and ROS scavenging by antioxidant compounds and enzymes. Under normal growth conditions, the production of ROS in cells is low (240 µM s−1 O2− and a steady state level of 0.5 H2O2 µM in chloroplast)  and ROS are normally in balance with antioxidant molecules. Non-enzymatic defenses include compounds of intrinsic antioxidant properties, water soluble such as ascorbate (vitamin C), glutathione, phenolic compounds and flavonoids, and lipid-soluble metabolites such as carotenoids and a-tocopherols (vitamin E). Antioxidants act as electron donors, reducing ROS to less harmful molecules; the oxidized products formed in the process are not very reactive or harmful. Purely enzymatic defenses include a variety of scavengers. Here, I have focused mainly on superoxide dismutase (SOD), catalase (CAT) ascorbate peroxidase (APX) and glutathione peroxidase, (GPX), which protect by directly scavenging superoxide radicals and hydrogen peroxide, converting them to less reactive species (Table 1). Homeostatic defenses can be overwhelmed in stress conditions, which increase ROS production (240–720 µM s−1 O2− and a steady state level of 5–15 H2O2 µM) . To reduce the damaging effects of ROS, aerobic organisms up-regulate both the non enzymatic and enzymatic antioxidant defenses .
2.1. Enzymatic Component of ROS Scavenging Defenses
2.1.1. Superoxide Dismutase (SODs)
Superoxide dismutase (SOD: EC 184.108.40.206) belongs to a class of metalloproteins, which catalyze the dismutation of superoxide (O2−) into molecular oxygen (O2) and H2O2 [2O2− + 2H+→O2 + H2O2]. The peroxide product must then be destroyed by CAT and/or peroxidases:
|O2•− + O2•− + 2H+||→||O2 + H2O2 (K2 = 2.4 × 109 M−1 s−1)|
|H2O2 + H2O2||→||2H2O + O2 (K1 = 1.7 × 107 M−1 s−1)|
|H2O2 + R(OH)2||→||2H2O + R(O)2 (K4 = 0.2–1 × 103 M−1 s−1)|
Thus, SOD and CAT serve in tandem as front-line antioxidant defenses. Therefore, it is not surprising to find that SOD and CAT are present in multiple subcellular locations (Figure 1).
Three classes of SODs can be distinguished on the basis of the metal co-factor at the catalytic site: Cu/ZnSOD, MnSOD, and FeSOD . Plant cells generally contain Cu/ZnSODs in the cytosol and chloroplasts and possibly the extracellular space, FeSODs in chloroplasts, and MnSODs in the mitochondrial matrix and peroxisomes (Figure 1). Both Cu/ZnSODs and FeSODs are dimers, whereas MnSODs of mitochondria are tetramers. The analysis of deduced amino acid sequences suggests that the three types of SOD fall into two phylogenetic families: the Fe-MnSODs and the Cu/ZnSODs. Mn and FeSODs are the more ancient type; they probably evolved from the same ancestral enzyme, whereas Cu/Zn-SODs have no detectable sequence similarity to Mn and FeSODs. They might have evolved separately in eukaryotes  and have been selected in response to a common environmental stress: the oxygenation of the biosphere by photosynthetic organisms .
Unlike most other organisms, which have only one of each type of SOD in the different cell compartments, plants have multiple forms of each type encoded by more than one gene. The expansion of the gene families reflects the complex antioxidant defenses of plants. The three SOD isoenzymes are nuclear-encoded by small multigene families. SOD multigene families have been widely studied and several SOD genes have been cloned in a variety of plant species such as A. thaliana , maize , tobacco [13,14], tomato , and rice [16,17,18]. In the A. thaliana genome, the Cu/ZnSOD family includes three genes (CSD1, CSD2 and CSD3), the FeSOD family includes three genes (FSD1, FSD2 and FSD3), while only one gene encodes MnSOD .
In maize, the SOD multigene family consists of at least ten different isoforms: five are Cu/ZnSOD (Sod1, Sod2, Sod4/Sod4A, Sod5 and Sod9), one is FeSOD (SodB) and four are MnSOD (Sod-3.1, Sod-3.2, Sod-3.3 and Sod-3.4) . The SOD gene family in maize seems more complex than it was earlier suggested and contains at least four additional not previously described genes: three FeSOD and one Cu/ZnSOD gene .
|Non enzymatic antioxidant molecules||Location||Primary ROS|
|Ascorbate (vitamin C)||Chl, Cyt, Mit, Per, Apo||H2O2, O2•−|
|Glutathione reduced (GSH)||Mit, Nuc, Per, Chl, Cyt, Apo Vac,||H2O2|
|α-tocopherol (vitamin E)||Cell and plastid membrane||ROOH, 1O2|
|Superoxide dismutase (SOD)||220.127.116.11||Cyt, Apo, (Cu/ZnSOD);Chl, (Cu/ZnSOD; FeSOD); Mit, (MnSOD); Per, (Cu/ZnSOD)||O2•−|
|Ascorbate peroxidase (APX)||18.104.22.168||Chl, Cyt, Mit, Per, Apo||H2O2|
|Peroxidase (non-specific)||22.214.171.124||Cyt; CW||H2O2|
|Glutathione peroxidase (GPX)||126.96.36.199||Cyt, Mit,||H2O2, ROOH|
|Glutathione reductase (GR)||1. 6.4.2||Mit, Cyt, Chl, Per||ROOH|
Abbreviations: Apo, apoplast; Chl, chloroplast; CW, cell wall; Cyt, cytosol; Mit, mitochondria; Nuc, nucleus; 1O2, singlet oxygen; Per, peroxisome; Vac, vacuole.
In poplar, 12 genes were identified in the Populus trichocarpa genome (Phytozome) by BLAST using as queries Arabidopsis and poplar sequence functionally annotated as SODs in the NCBI data base. Seven SODs were classified as Cu/ZnSODs in three strongly supported sub-groups (PtCSD1, PtCSD2 and PtCSD3) corresponding to their putative A. thaliana orthologs. The MnSOD group is the smallest of the three, with two members, PtMSD1 and PtMSD2, derived from genome-wide duplication. The FeSOD group of Populus contains three members as in Arabidopsis .
Recently, molecular studies have evidenced that SOD gene expression can be changed by alternative splicing [21,22] and microRNAs . Specialization of function among the different SOD isoenzymes could result from a combination of different subcellular locations and the features of gene regulation including promoter type and splicing.
To date, SODs have been characterized extensively for their physiological roles in protecting cells from toxic oxygen species in response to environmental stress such as ozone, drought, salinity, cold, etc. [13,24,25]. Increased transcript abundance is observed in response to abiotic and biotic stress to contrast oxidative stress exerts a significant role in stress tolerance. Transgenic plants over-expressing various SOD isoforms exhibit enhanced tolerance to oxidative stress and to various environmental stress factors; these results have been observed in many crops and model species including rice, potato, alfalfa, poplar, Arabidopsis, tobacco , etc. Moreover, SODs have been proved to be very sensitive to metabolic changes during cell life and respond to developmental stimuli. Induction of specific isoforms occurs in peroxisome during senescence [26,27], and transcription of MnSOD genes varies with both the ontogenetic stage and tissue growth rate [28,29].
Plant recalcitrance during in vitro culture might be associated with oxidative stress and ROS production. Strong induction of MnSOD and FeSOD enzymes was observed during somatic embryogenesis in horse chestnut , similarly high levels of SOD activity were necessary for embryogenic competence and development of somatic embryos of banana  and over-expression of Cu/ZnSOD enhanced in vitro shoot multiplication in transgenic plum .
2.1.2. Catalase (CATs)
Catalase (CAT: EC 188.8.131.52; 2H2O2 = 2H2O + O2) enzyme is ubiquitous in the peroxisome, where it dismutates H2O2 into water and oxygen. Together with SOD and hydroxyperoxidases, CATs are part of a defense system for scavenging superoxide radicals . The active CAT enzyme is a tetrameric iron porphyrin protein. In most eukaryotes, including mammals and several fungi, a single gene encodes the monomeric CAT subunits. In contrast, plant CATs are encoded by a small gene family, usually three or at most four isoenzyme genes in one species . In Nicotiana plumbaginifolia  and maize , each CAT isoenzyme shows a distinct spatial and temporal pattern of expression throughout the entire life cycle of the plant. Different classes of CATs may be determined on the basis of their expression profile and a specific nomenclature has been adopted for this classification . Class I CATs are expressed in photosynthetic tissues, synthesis is light-dependent, and the enzyme is involved in removal of H2O2. The class I includes the SU2 of cotton, Cat1 of N. plumbaginifolia, CAT2 of A. thaliana , Cat2 of Zea mays  and Cat1 of Prunus persica . Class II catalases are mainly found in vascular tissues and include the Cat2 of N. plumbaginifolia, Cat2 of castor bean , CatA of rice a homolog to the maize Cat3 , a tomato catalase , Cat2St from potato , CAT1 from A. thaliana  and Cat2 of peach . Class III CATs are mainly involved in the removal of H2O2 from glyoxysomes, and they are highly abundant in seeds and in young seedlings, while being almost absent at later stages of development (Table 2).
|Table 2. Classification of plant catalases based on expression properties a. Modified from Willekens et al. .|
|Species||Class I||Class II||Class III|
|Gossypium hirsutum (cotton)||SU2||SU1|
|Ricinus communis L. (castor bean)||CAT2||CAT1|
|Zea mays (maize)||CAT-2||CAT-3||CAT-1|
|Lycopersicon esculentum (tomato)||TOMCAT1|
|Solanum tuberosum (potato)||Cat2St|
|Prunus persica (peach)||Cat1||Cat2|
a Class I, expression in photosynthetic tissues, positively regulated by light; Class II, highest expression in vascular tissues; Class III, expression in seeds and young seedlings. Catalase names are reported as originally defined by the authors. Roman letters indicate a protein, and italics indicate a gene name.
Biochemical and molecular aspects of the complex roles of CATs have been thoroughly studied in herbaceous plants, both monocotyledons [36,39,42] and dicotyledons such as tomato , potato , cotton , Nicotiana plumbaginifolia [46,47], castor bean , barley , and A. thaliana . In woody plants, the biochemical aspects of catalase have been studied in gymnosperms [50,51], horse chestnut , oak , and peach . The spatiotemporal specificity that characterizes CAT isoform expression provided the rationale for using them as indicators of crucial developmental processes in woody species, such as somatic embryo conversion or rooting ability of microcuttings. In cypress, different catalase isoenzymes are linked to foliar dimorphisms of juvenile and mature shoots  and, similarly, in oak a specific catalase isoform was found specifically related to rooting, thus working as sensitive indicators of stress conditions during micro-propagation . Plants use catalases in pathways other than those common to other eukaryotes. Catalase activity is a determining factor for the protection of photosynthetic cells against oxidative stress induced during abiotic stress conditions such as chilling, drought, salt and ozone [37,46,53]. Catalases (Figure 2D), together with ascorbate peroxidase, are a key enzyme in modulating the level of H2O2, which acts downstream of salicylic acid as a second messenger implicated in the signal transduction pathway that in plants leads to the development of systemic acquired resistance (SAR). The different affinities of the two enzymes for H2O2 (µm range for APX and mM range for CAT) suggest that they belong to two different classes of H2O2 scavenging enzymes: CATs might be responsible for removal of ROS during stress while APX might be responsible for the fine modulation of ROS during signaling .
2.1.3. Ascorbate Peroxidase (APX)
Ascorbate peroxidases (EC: 184.108.40.206) catalyze the reduction of hydrogen peroxide to water by using ascorbate as the electron donor. Consequently, APX plays a role similar to that of CAT; however, the two enzymes exhibit distinctive features. APX utilizes ascorbate (AsA) as specific electron donor to reduce H2O2 to water with the parallel generation of monodehydroascorbate (MDHA), a univalent oxidant of ascorbate. MDHA is spontaneously disproportionated to AsA and dehydroascorbate (DHA). DHA reductase (DHAR) utilizes glutathione (GSH) as an electron donor to regenerate AsA from its oxidized form, DAsA (Figure 2C). Thus, the ascorbate–glutathione cycle located in the stroma of the chloroplast represents the main pathway to prevent the accumulation of toxic levels of H2O2 in photosynthetic organisms [55,56]. In addition to AsA–GSH, the water–water cycle, because of the electron flow from water in photosystem II (PSII) to water in PSI that occurs in this process across thylakoid membranes, participates in ROS detoxification and the dissipation of excess photon energy (Figure 2A).
Many studies reporting molecular cloning and physiological function of APX isoenzymes highlight the crucial role of APX in antioxidant protection in photosynthetic organisms. Furthermore, recent expression studies in response to stress conditions and pathogen attack indicate the importance of APX activity in intracellular signaling by controlling the H2O2 concentration. APX isoenzymes are distributed in four different cell compartments: soluble isoforms are found in the cytosol (cAPX), mitochondria (mitAPX) and chloroplast stroma (sAPX), while membrane-bound isoforms are found in peroxisomes and glyoxisomes (mAPX) and in chloroplast thylakoids (tAPX).
These enzymes are encoded by small gene families and expression of APX encoding genes is spatially and developmentally regulated and modulated by various environmental stimuli. Genomic and cDNA APX sequences are available from many plant species, showing that APXs are widely distributed in the vegetal kingdom. In the A. thaliana genome, the APX gene family includes nine genes encoding for isoenzymes found in the cytosol (APX1, APX2, APX6), chloroplast (APX3, APX4, APX6), mitochondrion (APX6, APX7), and peroxisome (APX3, APX5) plus one thylakoid-bound APX and one APX whose product is targeted to both chloroplast stroma and mitochondria . In rice, the APX gene family includes eight members, namely: two cytosolic, two peroxisomal, two chloroplastic (stromal and thylakoid-bound), and two mitochondrial ones [58,59].
APX genes from spinach, pea, tomato, strawberry, and eucalyptus have been isolated and characterized. Particular attention has focused on the plant chloroplastic APX (tAPX) genes that are divided into two groups according the regulatory mechanism of expression. The first group comprises single genes from spinach (Spinacia oleracea), tobacco (Nicotiana tabacum), pumpkin (Cucurbita spp.), and ice plant (Mesembryantheum crystallinum) encoding two isoenzymes through a post-transcriptional alternative splicing regulation of the two final exons . In the second group, single genes codify different isoenzymes, which are individually regulated. Genes from A. thaliana, rice and tomato belong to this group. APX plays a cooperative role in protection of each organelle. High and low temperatures, high light, drought and salt stress, heavy metals and pathogen attack modulate APX gene transcription . The over-expression in transgenic plant of different APX genes has defined the specific action of single genes in stress tolerance. Transgenic rice plants over-expressing a cytosolic APX1 gene (OsAPXa), exhibited enhanced cold tolerance at the booting stage . The over-expression in tobacco of a tomato chloroplastic tAPX gene was effective in minimizing photo-oxidative damage during high and low temperature stress [63,64]. The APX1 gene encoding cytoplasmic enzyme appears to be important in the acclimation of plants to combined stresses such as drought and heat . Indeed, when cytosolic APX was over-expressed in tobacco chloroplasts, the plant was protected from several stresses, including osmotic . The overexpression in transgenic tobacco of a peroxisomal ascorbate peroxidase poplar gene (PpAPX) improved plant drought resistance . In transgenic tobacco plants, over-expression of the thylakoid-bound APX (StAPX) gene of tomato enhanced the tolerance to salt and osmotic stress . A. thaliana mutants lacking cytosolic APX1 or tAPX and double mutants, i.e., tylapx/apx1 were generated to study how different ROS signals, generated in different cellular compartments, are integrated in cells. The plants deficient in two APX genes were late flowering, and exhibited low protein oxidation during light stress and enhanced anthocyanin synthesis. These results suggest that the lack of conventional ROS removal enzymes such as cytosolic and chloroplastic APXs is compensated by a high degree of plasticity in ROS signaling and the existence of redundant pathways for ROS protection .
Other enzymatic defenses, such as peroxiredoxins (PRXs), which are a family of thioredoxin-dependent peroxidases, are also widely distributed in plant cells. They represent another antioxidant enzyme system that would join catalase and the ascorbate-glutathione cycle in the control of H2O2, particularly in the plant exposed to different types of biotic and abiotic stresses. In addition to their role in antioxidant defense mechanisms, PRXs also modulate redox signaling during development and acclimation. The most updated information on the structure and catalysis of Prx and their functional significance in plant metabolism has been recently reviewed by Dietz .
2.2. Antioxidant Molecules
Antioxidants inhibit the oxidation of other molecules and thus prevent them from causing oxidative damage. According to Halliwell and Gutteridge , mechanisms of antioxidant action can include: (1) suppressing ROS formation either by inhibition of enzymes or chelating trace elements involved in free radical production; (2) scavenging ROS and up-regulating antioxidant defenses . Antioxidant molecules, which represent a second line of defense against ROS, include hydrophilic molecules (ascorbic acid, glutathione, phenolic compounds) and lipid-soluble metabolites (carotenoids and α-tocopherol) . Ascorbic acid (AsA) and glutathione (GSH), which are coupled to each other through the ascorbate glutathione cycle, as above described, are major water-soluble antioxidants and Redox buffers in plant cells. These key metabolites, which are present at high (millimolar) concentration in chloroplasts and other cellular compartments, have also crucial functions in stress responses and organ development [71,72].
Ascorbic acid (vitamin C) is synthesized in the mitochondria and is transported to the other cell compartments through a proton-electrochemical gradient or facilitated diffusion. Ascorbic acid is considered as the most powerful plant cell antioxidant because of its ability to donate electrons in a number of enzymatic and non enzymatic reactions. It can also directly scavenge O2•− and OH• and regenerate oxidized carotenoids or α-tocopherol, thus providing membrane protection  and minimizing the damage caused by oxidative process through synergic action with other antioxidants. High concentration is maintained by an efficient recycling system that makes AsA a convenient antioxidant. Ascorbate is present in all plant tissues, generally being higher in photosynthetic cells, meristems, and in some fruits. The analysis of subcellular distribution of reduced and oxidized ascorbate in leaf cells of A. thaliana and N. tabacum was recently performed by high-resolution immune electron microscopy . The nuclei and the cytosol of both plant species showed the highest concentration of ascorbate labeling, whereas mitochondria and plastids contained intermediate levels. The lowest ascorbate levels were found in the vacuoles. Lastly, the apoplastic ascorbate pool is important for control of cell elongation and triggering signal transduction cascades in response to external stimuli .
Glutathione is a thiol-containing tripeptide (glutamic acid-cysteine-glycine) that occurs in reduced form in all plant cell compartments: cytosol, endoplasmic reticulum, vacuole, chloroplast, mitochondria, and apoplast. It is the main non-protein thiol cofactor for various detoxifying enzymes and participates in several physiological processes such as the regulation of sulphur transport; the storage, detoxification of xenobiotics; signal transduction; and the expression of stress defense genes [72,75]. The main mechanisms controlling its concentration are the regulation of biosynthesis by γ-glutamylcysteine synthetase (γECS), the enzyme catalyzing the first step of glutathione biosynthesis [76,77,78]. GSH is essential for the antioxidant defense system particularly in chloroplasts, because it protects the photosynthetic apparatus counteracting the damaging effects of ROS. The cellular concentration of GSH varies considerably under biotic stresses and strong evidences correlate the ability of plant to withstand metal induced oxidative stress with high GSH concentrations [79,80].
GSH reacts chemically with a range of ROS while enzyme-catalyzed reactions link GSH to detoxification of H2O2 in the ascorbate-glutathione cycle (Figure 2C). Glutathione furthermore prevents denaturation of proteins caused by oxidation of thiol groups during stress. These functions results in the oxidation of GSH to form glutathione disulphide (GSSH). The ratio of GSH:GSSH is maintained by glutathione reductase (GR), which uses NAPDH to reduce GSSH to two GSH (Figure 2B,C). Beyond functioning in the ascorbate–glutathione cycle, GSH acts as direct electron donor to peroxides in reactions catalyzed by glutathione peroxidase (GPX). Unlike the constitutive GPXs of animals, plant GPXs are a large family of diverse isoenzymes, that are not constitutive but are stress induced; they do not contain selenium and catalyze GSH-dependent reduction of H2O2 at very low rates if compared to high rates of hydrogen peroxide production in plants . Consequently, while APXs and CATs are predominant in H2O2 detoxification, GPXs may play a role in other parts of antioxidant metabolism, including the removal of lipid peroxides. GPXs generally increase in plants subjected to environmental constraints such as during fungal infection, water deflicit, and metal stress, and decreases during photo-oxidative stress. This finding demonstrates that GPXs are involved in the response to both biotic and abiotic stress conditions .
Among the many antioxidant molecules, flavonoids and carotenoids are particularly interesting because they are common in fruits and relevant for human health. Phenolic compounds represent an important class of water soluble antioxidants. They are widespread, often at high levels, in almost all plant foods as flavonoids, tannins, and lignans. The most highly studied of these compounds are the flavonoids—a large class of more than 9000 structurally diverse compounds—which are synthesized by plants via the phenylpropanoid pathway. They include chalcones, flavones, flavonols, flavanones, anthocyanins, and isoflavonoids . The flavonoid basic structure is relatively simple and consists of 15 carbon atoms arranged in two aromatic C6 rings connected by a C3 unit; the rings are labeled A, B and C (Figure 3).
Flavonoid biosynthesis has been intensively studied in genetically tractable species as maize, snapdragon, and petunia; all the genes encoding the biosynthetic enzymes and transcription factors have been isolated and characterized. Many studies have been performed to verify the relationship between flavonoid structure and activity as ROS scavenger. Radical scavenging activity relies on the structure and the substituent of the heterocyclic ring. The catechol structure in the B ring, in particular, is the major determinant for flavonoid scavenging capacity .
Flavonoids commonly occur in plants, and many are glycosylated derivatives stored in the vacuole; besides vegetables and fruits, these compounds are found in seeds, nuts, grains, spices and different medicinal plants. The flavones apigenin and lutoelin are found in cereals and aromatic herbs, while the flavanones including hesperetin and naringenin are mainly found in citrus fruits. The flavonols quercitin and kaempferol are widespread in vegetables and in the skin of fruits (Figure 4). Antocyanidins and their glycosides antocyanins are abundant in berries and red grape.
Distribution among plants, structural aspects and antioxidant activity of most common flavonoids are extensively reviewed by Pietta . Flavonoids are involved in the resistance to plant pathogens and also provide a number of unrelated functions in the ecology of plants. Because of their attractive colors, flavones, flavonols and anthocyanidins play an important role in the attraction of pollinator insects and fruit eating animals. The catechins and other flavanols are bitter and may play a role in defence mechanisms against insects and predators, and furthermore they act as scavengers of ROS produced by the photosynthetic electron transport system in plant cells.
Several flavonoids proficiently chelate trace metals that play a role in oxygen metabolism. Many flavonoids biosynthetic genes are induced under stress conditions. Drought, chilling, wounding, metal toxicity and nutrient deficiency increase flavonoids levels . Moreover, due to their favorable UV-absorbing capacity, flavonoid compounds scavenge UV-generated ROS and protect plants against harmful UV radiation by absorption of this harmful radiation .
Carotenoids, which represent the main lipophilic antioxidants, are isoprenoid-derived molecules synthesized by plants, bacteria, fungi, and prokaryotic algae. Carotenoids are 40-carbon isoprenoids with polyene chains that may contain up to 15 conjugated double bonds. Based on their composition, carotenoids are divided into two classes: carotenes contain only carbon and hydrogen atoms while xanthophylls are the oxygenated forms of carotenes . Plant carotenoids are generally synthesized and located in plastids, and their production is driven by nuclear-encoded enzymes. The synthesis in plants follows this biosynthetic sequence: phytoene→phytofluene→ζ-carotene→neurosporene→lycopene→α-carotene and β-carotene (Figure 5).
Each enzymatic step from phytoene to lycopene adds one double bound to the molecule. Lycopene is a symmetrical molecule containing 13 double bonds. Enzymatic cyclization of lycopene marks a pathway branching point: one branch leads to β-carotene and its derivative xanthophylls, while the other leads to α-carotene and lutein . The carotenoid biosynthetic pathway has been extensively studied, and all the genes encoding the biosynthetic enzymes have been isolated and characterized from various organisms .
There are hundreds of carotenoids and most of them exhibit antioxidant activity. The best documented antioxidant actions of carotenoids are their abilities to quench singlet molecular oxygen and to trap peroxyl radicals. The antioxidant activity of carotenoids is influenced by the pattern of conjugated double bonds in the polyene backbone. In fact, it is the number of conjugated double bonds present in the molecule that determines the efficiency for physical quenching of 1O2. β-Carotene, zeaxanthin, cryptoxanthin, and α-carotene belong to the group of highly active quenchers of singlet molecular oxygen .
Because of its importance in human health by preventing a range of diseases, the bioavailability of provitamin A carotenoids and their bio-efficacy have been extensively studied. In particular, stable isotope methodology has advanced our knowledge. Presently, the relative mean conversion factors of β carotene into vitamin A by mammals are calculated by weight as 12:1 for orange fruit and that of β carotene from vegetables as 26:1. Nevertheless, the food matrix plays an important role in carotenoid bioavailability. For example, a certain amount of fat is needed to ensure the absorption of fat-soluble provitamin A. Furthermore, a large variation in bioconversion of dietary β-carotene was observed in relation to genetic variability in β carotene metabolism on individual human subjects .
Approximately 50 carotenoids are called “provitamin A” compounds, because the body can convert them into retinol, an active form of vitamin A. These (particularly β-carotene) represent the major dietary source of vitamin A. β-Carotene and lycopene have been best studied because they are the most common carotenoids in fruits and vegetables. These pigments cover a wide range of functions: photo-protective function against oxidative damage during photosynthesis, contribution to plant cross-talk with symbiotic organisms, and modulation of interactions with pests and pathogens .
More than 800 carotenoid species with a linear or cyclic structure have been discovered in plants. The carotenoid composition varies from species to species, and the accumulation of specific carotenoids in fruit and flower chromoplasts is a highly, developmentally regulated process . Consequently, their concentrations in fruits and vegetables vary with plant variety, degree of ripeness, time of harvest, growing and storage conditions. The orange-colored fruits and vegetables including carrots, pepper, apricots, mangoes, squash, papaya, and sweet potatoes contain significant amounts of β-carotene, α-carotene, and β-cryptoxanthin. Green vegetables, especially spinach, contain β-carotene and are the best sources of lutein. Lycopene produces the red color of tomatoes, and significant carotenoid variations are observed during fruit ripening .
Tocopherols are lipid-soluble antioxidant synthesized by all plants. They protect lipids and other membrane components by scavenging and quenching various ROS and lipid by-products of oxidative stress. Out of four isomers of tocopherols present in plants, α-tocopherol has the highest antioxidant activity and represents the major vitamin E compound; it is the only tocopherol absorbed efficiently by humans. α-Tocopherol is located in the chloroplast envelope and thylakoid membranes; high levels have been found in leaves of many plant species. Moreover, given that α-tocopherol increases membrane rigidity, its concentration, together with that of the other membrane components, may be regulated to afford adequate fluidity for membrane function. α-Tocopherol levels change significantly during plant growth and development, and in response to environmental stress. A number of reports deal with the increase in tocopherol in relation to chilling tolerance and water stress. Recently, activation of synthesis during oxidative stress was demonstrated to reflect activation of the pathway related genes. Furthermore, recent studies on tocopherol-deficient plants indicate that α-tocopherol may affect cellular signaling in plants [94,95,96,97].
Ascorbic acid, flavonoids, carotenoids, and tocopherols, because of their antioxidant properties and health promoting effects, represent attractive targets for bio-fortification programs. The increase of specific antioxidant products, both with molecular or conventional approaches, is an important and interesting topic for plant biotechnology and breeding. A variety of genetic strategies can be used to modify plant metabolism to enhance the availability of these compounds. The most popular are the over-expression of a known rate-limiting enzyme or the repression of a competitive pathway to canalize flux toward the desired metabolite. Furthermore, bioaccumulation (the amount of a particular nutrient that can be accumulated in plant tissues) represents an important but not ultimate goal. In fact, bioavailability (the amount that can be absorbed after consumption) of nutrients in crops is a more important indicator of its nutritional quality. Reducing anti-nutritional compounds and/or increasing the quantities of nutritional enhancers can therefore increase the bioavailability of nutrients. The bio-fortification strategies, which exceed the aims of this article, have been recently reviewed by Zhu and co-workers .
3. Antioxidant System during Development, Maturation and Fruit Ripening with Particular Attention to Prunus and Citrus spp.
The critical role exerted by ROS in processes involved in protecting plants from biotic and abiotic stresses has been the subject of extensive studies. Moreover, ROS are also acknowledged as important regulators of plant growth and development. For example, proper maintenance of ROS homeostasis is a crucial step for successful regeneration in the early stages of shoot organogenesis in sweet orange (Citrus sinensis) . There is a tremendous range of diverse processes in which ROS have been implicated acting as regulators of cytoplasmic signaling or as modulators of cell wall properties [100,101,102,103]. Recent insights into ROS biogenesis in a developmental context are discussed by Swanson and Gilroy using as examples the elongation of the root and the tip growing systems .
Fruit ripening, maturation, and senescence are developmental processes strongly related to agricultural production and are consequently of great interest to both crop physiologists and geneticists. Fruit development and ripening are dynamic events that involve a complex series of molecular and biochemical changes that result in modifying the complex network of metabolites and proteins. Biochemical processes include the degradation of chlorophyll and starch, the biosynthesis of pigments and volatile compounds, the accumulation of sugars and organic acids, as well as cell wall softening [104,105]. Nowadays, particularly through proteomic and metabolomic analysis during ripening stages, the first high-resolution picture of the metabolic dynamics during fruit development is available. Differential proteomic studies at different ripening stages, from immature to mature fruits, at the level of the whole fruit, but also with isolated flesh, seeds and skin, have been accomplished. Both climacteric fruit as tomato (L. esculentum) [106,107], peach (P. persica L. Batsch) [108,109,110,111,112,113,114], apple (Malus domestica)  and non-climacteric fruits, as grape (Vitis vinifera) [116,117,118,119,120,121,122,123,124], strawberry (Fragaria spp.) [125,126] and Citrus spp.  have been investigated with proteomics. The combination of protein separation methods (2DE, 1-DE, LC, DIGE and 2-DE plus western blotting) accompanied by MS identification provides a dynamic picture of fruit biology . These investigations offered significant information on molecular components involved in fruit ripening, including sugar metabolism, ethylene biosynthesis and perception, cell wall depolymerisation, signal transduction, stress responses, and enhanced accumulation of reactive oxygen species (ROS). Fruit ripening is an aerobic process generating ROS; these molecules initiate and enhance degenerative processes associated with fruit maturation, which is considered a protracted form of senescence . During ripening and senescence, some of the changes in the physical and chemical characteristics of fruit are not so easily observable, but impact fruit quality, while others, in addition to the perceivable alterations in fruit aroma, pigmentation, texture, and sweetness . Proteomic studies are contributing a deeper understanding of fruit maturation also in relation to post-harvest quality and shelf-life feature of the fruit. This is particularly important for fleshy fruits, such as peaches and apricots that are extremely susceptible to handling and physical injuries and require a long storage period before consumption of the ripe fruit. During post-harvest, the proteins related to stress response, cellular homeostasis, carbohydrate and amino acid metabolism were affected and some of them changed after low temperature storage . Ripening and senescence result in ROS accumulation and changes in antioxidant activity in climacteric fruits, i.e., tomato  and peach , characterized by a burst of ethylene production and respiratory activity. The involvement of ROS in ripening and post-harvest senescence in peaches was confirmed by studies using 2-DE and immunoblotting performed with isolated mitochondria. The results indicated that oxidative damage to mitochondria caused by ROS resulted in protein carbonylation of specific mitochondrial proteins such as MnSOD. The oxidative modification and loss of catalytic activity of the antioxidative enzyme, in turn, facilitated the release of superoxide radicals in mitochondria thus linking oxidative damage to fruit senescence . In orange (C. sinensis), several protein changes characterize stage III (the ripening and maturation stage). In this later stage of development, beside the many proteins related to sugar metabolism, those involved with ROS metabolism including GSH, PX, GST, CAT, SOD and APX were also increased . This last enzyme participates in the metabolism of ascorbate (vitamin C), an important constituent of orange fruits . Proteins related to antioxidant defense were also found particularly expressed in ripening blood sweet oranges (C. sinensis cv. Moro), which are characterized by red pigmentation of flesh and rind, in comparison with the common cultivar, highlighting their value for human health and nutrition .
The study of antioxidants in fruits is important for several reasons. Fruits are a significant part of our daily diet and increasing fruit consumption is an easy and logical strategy to increase antioxidant intake and decrease oxidative stress. Adding antioxidants to the human diet could reduce diseases such as cancer. The growing interest in nutraceuticals and functional foods has driven plant breeders to consider selection of crops with higher antioxidant contents in various common fruits. In addition to their relevance in human health, antioxidants also contribute in the protection of fruit tissues from potentially toxic ROS , thus increasing crop stress tolerance.
Fruits contain many different antioxidant components in different mixture according the species. Vitamin C, vitamin E, or β carotene and phenolic compounds, in particular flavonoids, constitute the primary antioxidant resources of fruits. Therefore, it was of enormous interest to measure the content of bioactive compounds and the total antioxidant capacity in a fruit or vegetable. Consequently, studies on commonly consumed fruits supply information useful for breeders, nutritionists, and consumers [134,135,136,137]. Several methods have been developed to assess “total” antioxidant activity of fruits; these assays differ in their chemistries and sensitivity. A common assay is iron-based assays FRAP (ferric reducing antioxidant power) . ABTS+ [azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid)] assay and DDPH (1,1-diphenyl-2-picrylhydrazyl), which measure free radical capacity, are more sensitive than the iron-based assay [138,139,140]. The results, even if obtained with different methodologies, indicate that antioxidant capacity and the concentrations of bioactive molecules differ greatly among the fruit species. Variability in antioxidant composition exists also among and within cultivars, and it is influenced by climatic conditions, agronomic practices, and pre- and post-harvest conditions . Antioxidant capacity is generally associated with total phenolic content, which is likely a reasonable predictor for the cellular antioxidant activity of fruits . Nevertheless, it has been also suggested that if phenolic compounds could be one of the main components responsible for reducing capability, they could not be main components in free radical scavenging in the fruits analyzed. Because of synergic actions of the different molecules on antioxidant activity, the total content of bioactive compounds is now taken into account by crop breeders in addition to overall fruit quality and organoleptic properties, traditionally used for the selection of new cultivars. Peaches and oranges merit particular attention in this context, because of the importance that these species have for human nutrition and health in addition to their economic relevance for Mediterranean countries and in the Americas. Oranges and peaches plus nectarines are, after apples, the most important fruit crops in Europe with a production in 2011 of 6,273,864 and 4,329,917 tons, respectively . Peaches and nectarines have a lower antioxidant capacity than other fruits including strawberry or orange, however, they are nutritionally important because they are a popular summer fruit and two of the mostly widely consumed fruits. Fresh fruits are an excellent source of sucrose, citric and malic acids, carotenoids and lactones, as well as polyphenol and pectic substances, which determine fruit sensory quality and nutritive value [143,144]. The analysis of antioxidant systems and total content of antioxidant molecules—flavonoids, anthocyanins, carotenoids, and vitamin C—has been performed on ripe fruit of peach and nectarine cultivars commonly grown all over the world [145,146,147,148,149,150]. The results obtained with comparable methods are presented in Table 3.
|Table 3. Antioxidant molecule contents and relative antioxidant capacity (RAC) in fruit flesh of peach.|
|Compounds||Means ± SE|
|Abibi et al. ||Cantin et al. |
|Vitamin C||4.0 ± 0.1||3.7 ± 1.0|
|Total phenolics||32.6 ± 0.7||36.4 ± 11.0|
|Flavonoids||12.5 ± 0.6||8.8 ± 0.4|
|Anthocyanins||3.2 ± 0.2||3.0 ± 0.3|
|RAC||464.2 ± 12.5||405.0 ± 4.9|
Anthocyanins (Figure 6) generate the red color present on skin and in flesh strongly characterizing peach and nectarine fruit. In addition to their contribution to aesthetics, they have strong antioxidant properties in relation to their different hydroxylation and glycosilation . The main anthocyanins present in peach are cyanindin-3-glucoside and cyanidin-3–rutinoside .
Phenolic distribution is an important aspect of the overall phenolic composition and antioxidant capacity. These compounds are not uniformly distributed within the fruit tissues, being mainly located in the epidermis and sub-epidermis layers of the fruit . For example, in a detailed study of Californian stone fruits, Gil and co-workers  gave a complete picture of antioxidant capacity and content in phenolics, carotenoids and vitamin C, both in flesh and peel of the ripe fruit (Table 4). This study and those performed by scientists of other countries on different local varieties demonstrated the great variability existing in term of antioxidant capacity among the cultivars.
Phenolics are the only compounds that correlate with total antioxidant capacity; both hydroxycinnamic acid and flavan-3ols are strongly correlated to total phenolic levels. In contrast, anthocyanins and flavonols are primarily in the peel and are therefore not correlated to total phenolic contents. Considering all available data, the antioxidant capacity of a fruit is the result of synergism among the diverse mixture of antioxidant molecules reflecting cultivar and genotype. Camejo and co-workers studied the oxidative parameters and antioxidant enzymatic system, in particular the activities of SOD and the components of the ascorbate-glutathione cycle. They utilized peach fruits suitable for fresh consumption and for the processing industry at two maturation stages . An increased activity of APX, MDHAR, and GR and changes in SOD isoenzyme patterns were observed in mature fruits, illustrating the efficacy of the antioxidant system to cope with the oxidative process accompanying ripening.
The increase in protein, antioxidants, and activities of enzymes involved in antioxidant metabolism together with the decrease of oxidants including both superoxide radical (O2•−) and hydrogen peroxide (H2O2) support the idea that synthesis of antioxidants or redox modulators is induced during ripening to protect the fruit against oxidative damage that might otherwise result during fruit development and maturation. On the other hand, the antioxidant system greatly contributes to delaying the senescence process in harvested fruit. Similar analysis during orange pulp fruit ripening and maturation performed demonstrated a different dynamics in the antioxidant system . In fact, fruit maturation and ripening were accompanied by a general decrease of antioxidant enzyme activities (SOD, CAT, APX and DHAR, except GR) and by a decrease in both the reduced and oxidized forms of ascorbate and glutathione. The decline in the antioxidant system paralleled the decline of antioxidant activity (FRAP values) and consequently was correlated with increased oxidative stress associated with ripening of sweet orange fruits.
|Table 4. Antioxidant molecule contents in ripe fruit (flesh and peel) of peach and nectarine differing for flesh color. Modified from Gil et al. .|
|Ascorbic acid vitamin C
mg/100g fresh weight
|Nectarines white flesh||5–14|
|Nectarines yellow flesh||5–7|
|Peaches white flesh||6–8|
|Peaches yellow flesh||4–13|
mg/100g fresh weight
|Nectarines white flesh||14–102|
|Nectarines yellow flesh||18–54|
|Peaches white flesh||28–111|
|Peaches yellow flesh||21–61|
|Carotenoids||Nectarines white flesh||7–11|
|µg/100g fresh weight||Nectarines yellow flesh||80–157|
|Peaches white flesh||8–17|
|Peaches yellow flesh||95–197|
In Citrus fruit, beside the ROS scavenging enzymes, the non-enzymatic components included carotenoids, polyphenols, and vitamin C [154,155,156]. Carotenoids are the pigments responsible for the external and internal coloration of fruit of most citrus species; therefore, their content and composition have a strong impact on both the commercial and nutritional quality of the fruit. Differences in carotenoid contents and profiles were observed among tissues and species. The main carotenoids present in citrus are β-cryptoxanthin, lutein, zeaxanthin, lycopene, β-carotene, and α carotene. The presence of lycopene characterizes some citrus mutants such as the Star Ruby grapefruit and the orange varieties Vaniglia Sanguigno, Cara Cara, and “Hong Anliu”. Lycopene confers bright red coloration and superior carotenoids contents to the pulp compared to non mutant fruits . The most important flavonoid classes are the flavanones (Figure 4) and the anthocyanins (Figure 6), the latter present specifically and exclusively in red blood oranges. The most significant flavanones are hesperidin, narirutin, and didimin (Figure 7) whose contents in blood oranges are reported to be almost two to three times higher than blond oranges [158,159]. Anthocyanins are evident in rind, leaves and flowers of some citrus (as lemon and Papeda citrus) only during the first developmental stage. In citrus mature fruits, anthocyanins are exclusively expressed in red-blood orange, which are the Tarocco, the Moro (both native to Italy), and the Sanguinello (native to Spain) and their hybrids in which anthocyanins production is strictly linked to genotype and environmental conditions . In the blood orange juices, the most widespread anthocyanins are cyanidin-3-glucoside and cyaniding 3-(6″-malonyl) glucoside [161,162,163].
Vitamin C is one of the main nutrients in citrus fruit and their products are one of the principal suppliers of dietary vitamin C. Many factor influence vitamin C content in the fruit: the species and cultivar, rootstock, climate condition, maturity state and position of fruit on the tree, production factors, and harvest and storage conditions. Thus, because of many horticultural and climatic variables involved in growth, it is not surprising that wide ranges of vitamin C levels were reported in the different citrus fruit. On the basis of the data available, oranges are richest in vitamin C, followed by grapefruit, lemons, tangerines and limes. The approximate ranges for vitamin C of mandarin, expressed as mg/100 mL of juice, are 15–55 (tangerine, USA), 20–60 (Mediterranean) and 20–50 (Satsuma, Japan). In lemons and limes, vitamin C values in juice show ranges of about 20–60 mg/100 mL and about 15–45 mg/100 mL, respectively. The vitamin C content of grapefruit has a range from 25 to 60 mg/100 mL of juice. The wide vitamin C ranges might reflect that lemons and limes are genetically complex biotypes and are regarded as introgressed trihybrids. In oranges, differences in vitamin C level, ranging from 30 to 88 mg/100 mL of juice has been observed among varieties, but also seasonal variations were described. Orange juices from January to March are richer in vitamin C than juices from April to July . Furthermore, bioavailability of vitamin C is enhanced by co-presence with bioflavonoids. In fact, although natural and synthetic Vitamin C is chemically identical, the ascorbate in the citrus extract was found to be more bioavailable in human subjects . Because of the high amounts of vitamin C, anthocyanins and carotenoids in blood orange, particular attention was paid to antioxidant properties of the juice. In vivo studies conducted on healthy people have shown that red orange juice consumption determines a significant increase in plasma vitamin C beta-cryptoxanthin, zeaxanthin, and cyanidin-3-glucoside. These results suggest that blood orange juice, due to synergic effects of its compounds, represents a bioavailable source of antioxidants that positively affect the antioxidant defense system . As a consequence, special attention has been paid by Italian researchers to evaluation of the stability of ascorbic acid in red pigmented blood orange juice and its detrimental effect on color stability. In blood orange juices, vitamin C ranges between 40 and 80 mg/100 mL and in Tarocco cultivar it is about 70–80 mg/100 mL . Fruit storage for long periods could decrease vitamin C content. Lower ascorbic acid content was observed after 65 days in Tarocco and Moro oranges without compromising antioxidant properties of the fruits; hence, these cultivars represent an excellent source of phytochemicals with potential health benefits for dietary supplement.
Antioxidant defense machinery involved directly or indirectly with ROS scavenging in plants represents a powerful tool to counteract oxidative stress at the cellular level. These antioxidant systems, which are essential for plant defense to avoid damage from abiotic and biotic stress, also represent a valuable resource for human health. In fact, although we cannot live healthily forever, we can help our cells to ameliorate oxidative stress and its detrimental effects through consumption of natural antioxidant derived from plants. More than ever, consumers desire attractive and tasteful foods that are safe, natural, and health-promoting. The natural antioxidants present in fruits are the easy, pleasant and logical strategy to increase antioxidant intake simply through increasing fruit consumption in the daily diet.
The author thanks Virginia Walbot for a critical reading of the manuscript and apologizes for the many colleagues’ reports not included in this work.
Conflicts of Interest
The author declares no conflict of interest.
- Halliwell, B. Reactive species and antioxidants. Redox biology is fundamental theme of aerobic life. Plant Physiol. 2006, 141, 312–322. [Google Scholar] [CrossRef]
- Suzuki, N.; Koussevitzky, S.; Mittler, R.; Miller, G. ROS and redox signaling in the response of plants to abiotic stress. Plant Cell Environ. 2012, 35, 259–270. [Google Scholar] [CrossRef]
- Miller, G.; Suzuki, N.; Ciftci-Yilmaz, S.; Mittler, R. Reactive oxygen species homeostasis and signaling during drought and salinity stresses. Plant Cell Environ. 2009, 33, 453–467. [Google Scholar]
- Dowling, D.K.; Simmons, L.W. Life-history evolution reactive oxygen species as universal constraints in life history. Proc. R. Soc. B 2009, 276, 1737–1745. [Google Scholar] [CrossRef]
- Grassmann, J.; Hippeli, S.; Elstner, E.F. Plant’s defence and its benefits for animals and medicine: Role of phenolics and terpenoids in avoiding oxygen stress. Plant Physiol. Biochem. 2002, 40, 471–478. [Google Scholar] [CrossRef]
- Mittler, R.; Vanderauwera, S.; Gollery, M.; van Breusegem, F. The reactive oxygen gene network of plants. Trends Plant Sci. 2004, 9, 490–498. [Google Scholar] [CrossRef]
- Polle, A. Dissecting the superoxide dismutase-ascorbateglutathione-pathway in chloroplasts by metabolic modeling. Computer simulations as a step towards flux analysis. Plant Physiol. 2001, 126, 445–462. [Google Scholar] [CrossRef]
- Apel, K.; Hirt, H. Reactive oxygen species: Metabolism, oxidative stress and signal transduction. Annu. Rev. Plant Biol. 2004, 55, 373–399. [Google Scholar] [CrossRef]
- Alsher, R.; Erturk, N.G.; Heath, L.S. Role of Superoxide dismutase in controlling oxidative stress in plants. J. Exp. Bot. 2002, 53, 1331–1341. [Google Scholar] [CrossRef]
- Scandalios, J.G. Oxidative stress: Molecular perception and transduction of signals triggering antioxidant gene defenses. Br. J. Med. Biol. Res. 2005, 38, 995–1014. [Google Scholar] [CrossRef]
- Kliebenstein, D.J.; Monde, R.A.; Last, R.L. Superoxide dismutase in Arabidopsis: An eclectic enzyme family with disparate regulation and protein localization. Plant Physiol. 1998, 118, 637–650. [Google Scholar] [CrossRef]
- Fink, R.C.; Scandalios, J.G. Molecular evolution and structure: Function relationships of the superoxide dismutase gene families in angiosperms and their relationship to other eukaryotic and prokaryotic superoxide dismutases. Arch. Biochem. Biophys. 2002, 399, 19–36. [Google Scholar] [CrossRef]
- Bowler, C.; van Camp, W.; van Montagu, M.; Inzé, D. Superoxide dismutase in plants. Crit. Rev. Plant Sci. 1994, 13, 199–218. [Google Scholar]
- Van Camp, W.; Inzé, D.; van Montagu, M. The regulation and function of tobacco superoxide dismutases. Free Radic. Biol. Med. 1997, 23, 515–520. [Google Scholar] [CrossRef]
- Perl-Treves, R.; Galun, E. The tomato Cu/Zn superoxide dismutase genes are developmentally regulated and respond to light and stress. Plant Mol. Biol. 1991, 17, 745–760. [Google Scholar] [CrossRef]
- Sakamoto, A.; Ohsuga, H.; Tanaka, K. Nucleotide sequences of two cDNA clones encoding different Cu/Zn-superoxide dismutases expressed in developing rice seed (Oryza sativa L.). Plant Mol. Biol. 1992, 19, 323–327. [Google Scholar] [CrossRef]
- Kaminaka, H.; Morita, S.; Yokoi, H.; Masumura, T.; Tanaka, K. Molecular cloning and characterization of a cDNA for plastidic copper/zinc-superoxide dismutase in rice (Oryza sativa L.). Plant Cell Physiol. 1997, 38, 65–69. [Google Scholar]
- Kaminaka, H.; Morita, S.; Tokumoto, M.; Yokoyama, H.; Masumura, T.; Tanaka, K. Molecular cloning and characterization of a cDNA for an iron-superoxide dismutase in rice (Oryza sativa L.). Biosci. Biotechnol. Biochem. 1999, 63, 302–308. [Google Scholar] [CrossRef]
- Katyshev, A.I.; Rogozin, I.B.; Konstantinov, Y.M. Identification of New Superoxide Dismutase Transcripts in Plants by EST Analysis: Alternative Polyadenylation and Splicing Events in Computational Structural and Functional Genomics and Transcriptomics. In Proceedings of the Fifth International Conference on Bioinformatics of Genome Regulation and Structure BGRS, Novosibirsk, Russia, 16–22 July 2006; pp. 61–64.
- Molina-Rueda, J.J.; Tsai, C.J.; Kirby, E.G. The Populus superoxide dismutase gene family and its responses to drought stress in transgenic poplar overexpressing a pine cytosolic glutamine synthetase (GS1a). PLos One 2012, 8, e564211. [Google Scholar]
- Srivastava, V.; Srivastava, M.K.; Nilsson, R.; Rouhier, N.; Melzer, M.; Wingsle, G. Alternative splicing studies of the reactive oxygen species gene network in populus reveal two isoforms of high-isoelectric-point superoxide dismutase. Plant Physiol. 2009, 149, 1848–1859. [Google Scholar] [CrossRef]
- Feng, W.; Hongbin, W.; Bing, L.; Jinfa, W. Cloning and characterization of a novel splicing isoform of the iron-superoxide dismutase gene in rice (Oryza sativa L.). Plant Cell Rep. 2006, 24, 734–742. [Google Scholar] [CrossRef]
- Sunkar, R.; Kapoor, A.; Zhu, J.K. Posttranscriptional induction of two Cu/Zn superoxide dismutase genes in Arabidopsis is mediated by down regulation of miR398 and important for oxidative stress tolerance. Plant Cell 2006, 18, 2051–2065. [Google Scholar] [CrossRef]
- Gill, S.; Tuteja, N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol. Biochem. 2010, 48, 909–930. [Google Scholar] [CrossRef]
- Van Camp, W.; Willekens, H.; Bowler, C.; Van Montagu, M.; Inzé, D.; Reupold-Popp, P.; Sandermann, H., Jr.; Langebartels, C. Elevated levels of superoxide dismutase protect transgenic plants against ozone damage. Nat. Biotechnol. 1994, 12, 165–168. [Google Scholar] [CrossRef]
- Longa, M.A.; Del Rio, L.A.; Palma, J.M. Superoxide dismutases of chestnut leaves Castanea sativa: Characterization and study of their involvement in natural leaf senescence. Physiol. Plant. 1994, 92, 227–232. [Google Scholar] [CrossRef]
- Pastori, G.M.; Del Rio, L.A. Natural senescence of pea leaves an activated oxygen-mediated function for peroxisomes. Plant Physiol. 1997, 113, 411–418. [Google Scholar]
- Bagnoli, F.; Giannino, D.; Caparrini, S.; Camussi, A.; Mariotti, D.; Racchi, M.L. Molecular cloning, characterization and expression of a Mn-superoxide dismutase gene from peach (Prunus persica [L.] Batsch). Mol. Genet. Genomics 2002, 267, 321–328. [Google Scholar] [CrossRef]
- Swanson, S.; Gilroy, S. ROS in plant development. Physiol. Plant. 2010, 138, 384–392. [Google Scholar] [CrossRef]
- Bagnoli, F.; Capuana, M.; Racchi, M.L. Developmental changes of catalase and superoxide dismutase isoenzymes in zygotic and somatic embryos of horse chestnut. Aust. J. Plant Physiol. 1998, 25, 909–913. [Google Scholar] [CrossRef]
- Ma, L.; Xie, L.; Lin, G.; Jiang, S.; Chen, H.; Li, H.; Takac, T.; Samaj, J.; Xu, C. Histological changes and differences in activities of some antioxidant enzymes and hydrogen peroxide content during somatic embryogenesis of Musa AAA cv. Yueyoukang 1. Sci. Hortic. 2012, 144, 87–92. [Google Scholar] [CrossRef]
- Faize, M.; Faize, L.; Petri, C.; Barba-Espin, G.; Diaz-Vivancos, P.; Clemente-Moreno, M.J.; Koussa, T.; Hernandez, J.A. Cu/Zn superoxide dismutase and ascorbate peroxidase enhance in vitro shoot multiplication in transgenic plum. J. Plant Physiol. 2013, 170, 625–632. [Google Scholar] [CrossRef]
- Mittler, R. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 2002, 7, 405–410. [Google Scholar] [CrossRef]
- Scandalios, J.G. Oxidative Stress and the Molecular Biology of Antioxidant Defenses; Cold Spring Harbor Laboratory Press: New York, NY, USA, 1997. [Google Scholar]
- Willekens, H.; Langebartels, C.; Tiré, C.; van Montagu, M.; Inzé, D.; van Camp, W. Differential expression of catalase genes in Nicotiana plumbaginifolia (L.). Proc. Natl. Acad. Sci. USA 1994, 91, 10450–10454. [Google Scholar] [CrossRef]
- Scandalios, J.G. The Antioxidant Enzyme Genes Cat and Sod of Maize: Regulation, Functional Significance, and Molecular Biology. In Isozymes: Current Topics in Biological and Medical Research, Molecular and Cellular Biology; Rattazzi, M.C., Scandalios, J.G., Whitt, G.S., Eds.; Alan R. Liss: New York, NY, USA, 1987; pp. 19–44. [Google Scholar]
- Willekens, H.; Inzé, D.; van Montagu, M.; van Camp, W. Catalases in plants. Mol. Breed. 1995, 1, 207–228. [Google Scholar] [CrossRef]
- Frugoli, J.A.; Zhong, H.H.; Nuccio, M.L.; McCourt, P.; McPeek, M.A.; Thomas, T.L.; McClung, C. Catalase is encoded by a multigene family in Arabidopsis thaliana (L.) Heynh. Plant Physiol. 1996, 112, 327–336. [Google Scholar]
- Scandalios, J.G.; Guan, L.M.; Polidoros, A. Catalases in Plants: Gene Structure, Properties, Regulation, and Expression. In Oxidative Stress and the Molecular Biology of Antioxidant Defenses; Scandalios, J.G., Ed.; Cold Spring Harbor Laboratory Press: Plainview, NY, USA, 1997; pp. 343–406. [Google Scholar]
- Bagnoli, F.; Danti, S.; Magherini, V.; Cozza, R.; Innocenti, A.M.; Racchi, M.L. Molecular cloning, characterization and expression of two catalase genes from peach (Prunus persica). Funct. Plant Biol. 2004, 3, 349–357. [Google Scholar]
- Suzuki, M.; Ario, T.; Hattori, T.; Nakamura, K.; Asahi, T. Isolation and characterization of two tightly linked catalase genes from castor bean that are differentially regulated. Plant Mol. Biol. 1994, 25, 507–516. [Google Scholar] [CrossRef]
- Higo, K.; Higo, H. Cloning and characterization of the rice CatA catalase gene, a homologue of the maize Cat3 gene. Plant Mol. Biol. 1996, 30, 505–521. [Google Scholar] [CrossRef]
- Drory, A.; Woodson, W.R. Molecular cloning and nucleotide sequence of a cDNA encoding catalase from tomato. Plant Physiol. 1992, 100, 1605–1606. [Google Scholar]
- Niebel, A.; Heungens, K.; Barthels, N.; Inzé, D.; van Montagu, M.; Gheysen, G. Characterization of a pathogen-induced potato catalase and its systemic expression upon nematode and bacterial infection. Mol. Plant Microbe Interact. 1995, 8, 371–378. [Google Scholar] [CrossRef]
- Ni, W.; Trelease, R.N. Post-translational regulation of catalase isozyme expression in cottonseeds. Plant Cell 1991, 3, 737–744. [Google Scholar]
- Willekens, H.; Villarroel, R.; van Montagu, M.; Inzé, D.; van Camp, W. Molecular identification of catalases from Nicotiana plumbaginifolia (L.). FEBS Lett. 1994, 352, 79–83. [Google Scholar] [CrossRef]
- Willekens, H.; Chamnongpol, S.; Davey, M.; Schraudner, M.; Langebartels, C.; van Montagu, M.; Inzé, D.; van Camp, W. Catalase is a sink for H2O2 and is indispensable for stress defence in C3 plants. EMBO J. 1997, 16, 4806–4816. [Google Scholar] [CrossRef]
- Suzuki, M.; Miyamoto, R.; Hattori, T.; Nakamura, K.; Asahi, T. Differential regulation of the expression in transgenic tobacco of the gene for b-glucuronidase under the control of the 5′-upstream regions of two catalase genes from castor bean. Plant Cell Physiol. 1995, 36, 273–279. [Google Scholar]
- Skadsen, R.W.; Schulze-Lefert, P.; Herbst, J.M. Molecular cloning, characterization and expression analysis of two catalase isozyme genes in barley. Plant Mol. Biol. 1995, 29, 1005–1014. [Google Scholar] [CrossRef]
- Mullen, R.T.; Gifford, D.J. Purification and characterization of catalase from loblolly pine (Pinus taeda L.) megagametophytes. Plant Physiol. 1993, 103, 477–483. [Google Scholar]
- Racchi, M.L.; Chiusi, A.P.; Giannini, R. Catalase isozymes as biochemical markers of different developmental stages in cypress (Cupressus sempervirens). Can. J. For. Res. 1996, 26, 1629–1633. [Google Scholar] [CrossRef]
- Racchi, M.L.; Bagnoli, F.; Balla, I.; Danti, S. Differential activity of catalase and superoxide dismutase in seedlings and in vitro micro-propagated oak (Quercus robur L.). Plant Cell Rep. 2001, 20, 169–174. [Google Scholar] [CrossRef]
- Dat, J.; Vandenabeele, S.; Vranová, E.; van Montagu, M.; Inzé, D.; van Breusegem, F. Dual action of the active oxygen species during plant stress responses. Cell. Mol. Life Sci. 2000, 57, 779–795. [Google Scholar] [CrossRef]
- Davletova, S.; Rizhsky, L.; Liang, H.; Shengqiang, D.; Oliver, D.; Coutu, J.; Shulaev, V.; Schlauch, K.; Mittler, R. Cytosolic ascorbate peroxidase 1 is a central component of the reactive oxygen gene network of Arabidopsis. The Plant Cell 2004, 17, 268–281. [Google Scholar]
- Noctor, G.; Foyer, C.H. Ascorbate and glutathione: Keeping active oxygen under control. Annu. Rev. Plant Physiol. Plant Mol. Biol. 1998, 49, 249–279. [Google Scholar] [CrossRef]
- Asada, K. Production and Scavenging of Active Oxygen in Chloroplasts. In Current Communications in Cell and Molecular Biology 5. Molecular Biology of Free Radical Research Scavenging Systems; Scandalios, J.G., Ed.; Cold Spring Harbour Laboratory Press: New York, NY, USA, 1992; pp. 173–192. [Google Scholar]
- Chew, O.; Whelan, J.; Millar, A.H. Molecular definition of the ascorbate-glutathione cycle in Arabidopsis mitochondria reveals dual targeting of antioxidant defenses in plants. J. Biol. Chem. 2003, 278, 46869–46877. [Google Scholar] [CrossRef]
- Teixeira, F.K.; Menezes-Benavente, L.; Margis, R.; Margis-Pinheiro, M. Analysis of the molecular evolutionary history of the ascorbate peroxidase gene family: Inferences from the rice genome. J. Mol. Evol. 2004, 59, 761–770. [Google Scholar] [CrossRef]
- Teixeira, F.K.; Menezes-Benavente, L.; Galvão, V.C.; Margis, R.; Margis-Pinheiro, M. Rice ascorbate peroxidase gene family encodes functionally diverse isoforms localized in different subcellular compartments. Planta 2006, 224, 300–314. [Google Scholar] [CrossRef]
- Ishikawa, T.; Yoshimura, K.; Tamoi, M.; Takeda, T.; Shigeoka, S. Alternative mRNA splicing of 3′-terminal exons generates ascorbate peroxidase isoenzymes in spinach (Spinacia oleracea) chloroplasts. Biochem. J. 1997, 328, 795–800. [Google Scholar]
- Caverzan, A.; Passaia, G.; Barcellos Rosa, S.; Werner Ribeiro, C.; Lazzarotto, F.; Margis-Pinheiro, M. Plant responses to stresses: Role of ascorbate peroxidase in the antioxidant protection. Genet. Mol. Biol. 2012, 35, 1011–1019. [Google Scholar] [CrossRef]
- Sato, Y.; Masuta, Y.; Saito, K.; Murayama, S.; Ozawa, K. Enhanced chilling tolerance at the booting stage in rice by transgenic over-expression of the ascorbate peroxidase gene, OsAPXa. Plant Cell Rep. 2011, 30, 299–406. [Google Scholar]
- Sun, W.H.; Duan, M.; Shu, D.F.; Yang, S.; Meng, Q.W. Over-expression of tomato tAPX gene in tobacco improves tolerance to high or low temperature stress. Biol. Plant. 2010, 54, 614–620. [Google Scholar] [CrossRef]
- Sun, W.H.; Duan, M.; Shu, D.F.; Yang, S.; Meng, Q.W. Over-expression of StAPX in tobacco improves seed germination and increases early seedling tolerance to salinity and osmotic stresses. Plant Cell Rep. 2010, 29, 917–926. [Google Scholar] [CrossRef]
- Koussevitzky, S.; Suzuki, N.; Huntington, S.; Armijo, L.; Sha, W.; Cortes, D.; Shulaev, V.; Mittler, R. Ascorbate peroxidase 1 plays a key role in the response of Arabidopsis thaliana to stress combination. J. Biol. Chem. 2008, 283, 34197–34203. [Google Scholar] [CrossRef]
- Badawi, G.H.; Kawano, N.; Yamauchi, Y.; Shimada, E.; Sasaki, R.; Kubo, A.; Tanaka, K. Over-expression of ascorbate peroxidase in tobacco chloroplasts enhances the tolerance to salt stress and water deficit. Physiol. Plant. 2004, 121, 231–238. [Google Scholar] [CrossRef]
- Li, Y.-J.; Hai, R.-L.; Du, X.-H.; Jiang, X.-N.; Lu, H. Over-expression of a Populus peroxisomal ascorbate peroxidase (PpAPX) gene in tobacco plants enhances stress tolerance. Plant Breed. 2009, 128, 404–410. [Google Scholar] [CrossRef]
- Miller, G.; Suzuki, N.; Rizhsky, L.; Hegie, A.; Koussevitzky, S.; Mittler, R. Double mutants deficient in cytosolic and thylakoid ascorbate peroxidase reveal a complex mode of interaction between reactive oxygen species, plant development, and response to abiotic stresses. Plant Physiol. 2007, 144, 1777–1785. [Google Scholar] [CrossRef]
- Dietz, K.J. Peroxiredoxins in plants and cyanobacteria. Antioxid. Redox. Signal. 2011, 15, 1129–1159. [Google Scholar] [CrossRef]
- Halliwell, B.; Gutteridge, J.M.C. Free Radicals in Biology and Medicine, 4th ed.; Oxford University Press: Oxford, UK, 2007. [Google Scholar]
- Arrigoni, O.; De Tullio, M.C. Ascorbic acid, much more than just an antioxidant. Biochim. Biophys. Acta 2002, 1569, 1–9. [Google Scholar] [CrossRef]
- Noctor, G.; Gomez, L.; Vanacker, H.; Foyer, C.H. Interactions between biosynthesis, compartmentation and transport in the control of glutathione homeostasis and signaling. J. Exp. Bot. 2002, 53, 1283–1304. [Google Scholar] [CrossRef]
- Zechmann, B.; Stumpe, M.; Mauch, F. Immunocytochemical determination of the subcellular distribution of ascorbate in plants. Planta 2011, 233, 1–12. [Google Scholar] [CrossRef]
- Pignocchi, C.; Kiddle, G.; Hernández, I.; Foster, S.J.; Asensi, A.; Taybi, T.; Barnes, J.; Foyer, C.H. Ascorbate oxidase-dependent changes in the redox state of the apoplast modulate gene transcript accumulation leading to modified hormone signaling and orchestration of defence processes in tobacco. Plant Physiol. 2006, 141, 423–435. [Google Scholar] [CrossRef]
- Xiang, C.; Werner, B.L.; Christensen, E.M.; Oliver, D.J. The biological functions of glutathione revisited in Arabidopsis transgenic plants with altered glutathione levels. Plant Physiol. 2001, 126, 564–574. [Google Scholar] [CrossRef]
- May, M.J.; Vernoux, T.; Sánchez-Fernández, R.; van Montagu, M.; Inzé, D. Evidence for posttranscriptional activation of γ-glutamylcysteine synthetase during plant stress responses. Proc. Natl. Acad. Sci. USA 1998, 95, 12049–12054. [Google Scholar] [CrossRef]
- Xiang, C.; Oliver, D.J. Glutathione metabolic genes coordinately respond to heavy metals and jasmonic acid in Arabidopsis. Plant Cell 1998, 10, 1539–1550. [Google Scholar]
- Hicks, L.M.; Cahoon, R.E.; Bonner, E.R.; Rivard, R.S.; Sheffield, J.; Jez, J.M. Thiol-based regulation of redox-active glutamate-cysteine ligase from Arabidopsis thaliana. Plant Cell 2007, 19, 2653–2661. [Google Scholar]
- Pietrini, F.; Iannelli, M.A.; Pasqualini, S.; Massacci, A. Interaction of cadmium with glutathione and photosynthesis in developing leaves and chloroplasts of Phragmites australis (Cav.) Trin. ex Steudel. Plant Physiol. 2003, 133, 829–837. [Google Scholar] [CrossRef]
- Sun, Q.; Yec, Z.H.; Wang, X.R.; Wong, M.H. Cadmium hyperaccumulation leads to an increase of glutathione rather than phytochelatins in the cadmium hyperaccumulator Sedum alfredii. J. Plant Physiol. 2007, 164, 1489–1498. [Google Scholar]
- Navrot, N.; Collin, V.; Gualberto, J.; Gelhaye, E.; Hirasawa, M.; Rey, P.; Knaff, D.B.; Issakidis, E.; Jacquot, J.-P.; Rouhier, N. Plant glutathione peroxidases are functional peroxiredoxins distributed in several subcellular compartments and regulated during biotic and abiotic stresses. Plant Physiol. 2006, 142, 1364–1379. [Google Scholar] [CrossRef]
- Harborne, J.B. Plant Flavonoids in Biology and Medicine; Cody, V., Middleton, E., Harborne, J.B., Eds.; Alan R. Liss: New York, NY, USA, 1986; pp. 15–24. [Google Scholar]
- Bors, W.; Heller, W.; Michel, C.; Saran, M. Flavonoids as antioxidants: Determination of radical-scavenging efficiencies. Methods Enzymol. 1990, 186, 343–355. [Google Scholar] [CrossRef]
- Pietta, P.G. Flavonoids as antioxidants. J. Nat. Prod. 2000, 63, 1035–1042. [Google Scholar] [CrossRef]
- Pourcel, L.; Routaboul, J.M.; Cheynier, V.; Lepiniec, L.; Debeaujon, I. Flavonoid oxidation in plants: From biochemical properties to physiological functions. Trend Plant Sci. 2007, 12, 29–36. [Google Scholar]
- Solovchenko, A. Photoprotection in Plants; Springer-Verlag: Berlin, Heidelberg, Germany, 2010; pp. 143–158. [Google Scholar]
- Hirschberg, J. Carotenoid biosynthesis in flowering plants. Curr. Opin. Plant Biol. 2001, 4, 210–218. [Google Scholar] [CrossRef]
- Lu, S.; Li, L. Carotenoid metabolism: Biosynthesis, regulation, and beyond. J. Integr. Plant Biol. 2008, 50, 778–785. [Google Scholar] [CrossRef]
- Stahl, W.; Sies, H. Antioxidant activity of carotenoids. Mol. Asp. Med. 2003, 24, 345–351. [Google Scholar] [CrossRef]
- Tang, G. Bioconversion of dietary Provitamin A carotenoids to Vitamin A in humans. Am. J. Clin. Nutr. 2010, 91, 1468S–1473S. [Google Scholar] [CrossRef]
- Paiva, S.A.; Russell, R.M. Beta-carotene and other carotenoids as antioxidants. J. Am. Coll. Nutr. 1999, 18, 426–433. [Google Scholar] [CrossRef]
- Giuliano, G.; Bartley, G.E.; Scolnik, P.A. Regulation of carotenoid biosynthesis during tomato development. Plant Cell 1993, 5, 379–387. [Google Scholar]
- Bramley, P.M. Regulation of carotenoid formation during tomato fruit ripening and development. J. Exp. Bot. 2002, 53, 2107–2113. [Google Scholar] [CrossRef]
- Munné-Bosch, S. Alpha-tocopherol: A multifaceted molecule in plants. Vitam. Horm. 2007, 76, 375–392. [Google Scholar] [CrossRef]
- Munné-Bosch, S. The role of alpha-tocopherol in plant stress tolerance. J. Plant Physiol. 2005, 162, 743–748. [Google Scholar] [CrossRef]
- Munné-Bosch, S.; Weiler, E.W.; Alegre, L.; Müller, M.; Düchting, P.; Falk, J. Alpha-tocopherol may influence cellular signaling by modulating jasmonic acid levels in plants. Planta 2007, 225, 681–691. [Google Scholar] [CrossRef]
- Hincha, D.K. Effects of alpha-tocopherol (vitamin E) on the stability and lipid dynamics of model membranes mimicking the lipid composition of plant chloroplast membranes. FEBS Lett. 2008, 582, 3687–3692. [Google Scholar] [CrossRef]
- Zhu, C.; Sanahuja, G.; Yuan, D.; Farre, G.; Arjo, G.; Berman, J.; Zorrilla Lopez, U.; Banakar, R.; Bai, C.; Perez-Massot, E.; et al. Biofortification of plants with altered antioxidant content and composition: Genetic engineering strategies. Plant Biotechnol. J. 2013, 11, 129–141. [Google Scholar] [CrossRef]
- Faltin, Z.; Holland, D.; Velcheva, M.; Tsapovetsky, M.; Roeckel-Drevet, P.; Handa, A.K.; Abu-Abied, M.; Friedman-Einat, M.; Eshdat, Y.; Perl, A. Glutathione peroxidase regulation of reactive oxygen species level is crucial for in vitro plant differentiation. Plant Cell Physiol. 2010, 51, 1151–1162. [Google Scholar] [CrossRef]
- Joo, J.H.; Bae, Y.S.; Lee, J.S. Role of auxin-induced reactive oxygen species in root gravitropism. Plant Physiol. 2001, 126, 1055–1060. [Google Scholar] [CrossRef]
- Muller, K.; Linkies, A.; Vreeburg, R.A.; Fry, S.C.; Krieger-Liszkay, A.; Leubner-Metzger, G. In vivo cell wall loosening by hydroxyl radicals during cress (Lepidium sativum L.) seed germination and elongation growth. Plant Physiol. 2009, 150, 1855–1865. [Google Scholar] [CrossRef]
- Pedreira, J.; Sanz, N.; Pena, M.J.; Sanchez, M.; Queijeiro, E.; Revilla, G.; Zarra, I. Role of apoplastic ascorbate and hydrogen peroxide in the control of cell growth in pine hypocotyls. Plant Cell Physiol. 2004, 45, 530–534. [Google Scholar] [CrossRef]
- Schopfer, P. Hydroxyl radical-induced cell-wall loosening in vitro and in vivo: Implications for the control of elongation growth. Plant J. 2001, 28, 679–688. [Google Scholar] [CrossRef]
- Giovannoni, J. Molecular biology of fruit maturation and ripening. Annu. Rev. Plant Physiol. Plant Mol. Biol. 2001, 57, 735–749. [Google Scholar]
- Giovannoni, J. Genetic regulation of fruit development and ripening. Plant Cell 2004, 16, S170–S180. [Google Scholar] [CrossRef]
- Carrari, F.; Baxter, C.; Usadel, B.; Urbanczyk-Wochniak, E.; Zanor, M.I.; Nunes-Nesi, A.; Nikiforova, V.; Centero, D.; Ratzka, A.; Pauly, M.; et al. Integrated analysis of metabolite and transcript levels reveals the metabolic shifts that underlie tomato fruit development and highlight regulatory aspects of metabolic network behavior. Plant Physiol. 2006, 142, 1380–1396. [Google Scholar] [CrossRef]
- Faurobert, M.; Mihr, C.; Bertin, N.; Pawlowski, T.; Negroni, L.; Sommerer, N.; Causse, M. Major proteome variations associated with cherry tomato pericarp development and ripening. Plant Physiol. 2007, 143, 1327–1346. [Google Scholar] [CrossRef]
- Lara, M.V.; Borsani, J.; Budde, C.O.; Lauxmann, M.A.; Lombardo, V.A.; Murray, R.; Andreo, C.S.; Drincovich, M.F. Biochemical and proteomic analysis of ‘Dixiland’ peach fruit (Prunus persica) upon heat treatment. J. Exp. Bot. 2009, 60, 4315–4333. [Google Scholar] [CrossRef]
- Nilo, R.; Saffie, C.; Lilley, K.; Baeza-Yates, R.; Cambiazo, V.; Campos-Vargas, R.; González, M.; Meisel, L.A.; Retamales, J.; Silva, H.; Orellana, A. Proteomic analysis of peach fruit mesocarp softening and chilling injury using difference gel electrophoresis (DIGE). BMC Genomics 2010, 11, 43. [Google Scholar] [CrossRef]
- Lombardo, V.A.; Osorio, S.; Borsani, J.; Lauxmann, M.A.; Bustament, C.; Budde, C.; Andreo, C.S.; Lara, M.V.; Alisdair, R.; Fernie Drincovich, M.F. Metabolic profiling during peach fruit development and ripening reveals the metabolic networks that underpin each developmental stage. Plant Physiol. 2011, 157, 1696–1710. [Google Scholar] [CrossRef]
- Zhang, L.; Yu, Z.; Jiang, L.; Jiang, J.; Luo, H.; Fu, L. Effect of post-harvest heat treatment on proteome change of peach fruit during ripening. J. Proteomics 2011, 74, 1135–1149. [Google Scholar] [CrossRef]
- Prinsi, B.; Negri, A.S.; Fedeli, C.; Morgutti, S.; Negrini, N.; Cocucci, M.; Espen, L. Peach fruit ripening: A proteomic comparative analysis of the mesocarp of two cultivars with different flesh firmness at two ripening stages. Phytochemistry 2011, 72, 1251–1262. [Google Scholar] [CrossRef]
- Hu, H.; Liu, Y.; Shi, G.L.; Wu, R.J.; Yang, A.Z.; Wang, Y.M.; Hua, B.G.; Wang, Y.N. Proteomic analysis of peach endocarp and mesocarp during early fruit development. Physiol. Plant. 2011, 142, 390–406. [Google Scholar] [CrossRef]
- Nilo, R.P.; Campos-Vargas, R.; Orellana, A. Assessment of Prunus persica fruit softening using a proteomics approach. J. Proteomics 2012, 75, 1618–1638. [Google Scholar] [CrossRef]
- Guarino, C.; Arena, S.; De Simone, L.; D’Ambrosio, C.; Santoro, S.; Rocco, M.; Scaloni, A.; Marra, M. Proteomic analysis of the major soluble components in Annurca apple flesh. Mol. Nutr. Food Res. 2007, 51, 255–262. [Google Scholar] [CrossRef]
- Sarry, J.E.; Sommerer, N.; Sauvage, F.X.; Bergoin, A.; Rossignol, M.; Albagnac, G.; Romieu, C. Grape berry biochemistry revisited upon proteomic analysis of the mesocarp. Proteomics 2004, 4, 201–215. [Google Scholar] [CrossRef]
- Giribaldi, M.; Perugini, I.; Sauvage, F.X.; Schubert, A. Optimization of protein extraction and solubilization for mature grape berry clusters. Proteomics 2007, 7, 3154–3170. [Google Scholar] [CrossRef]
- Deytieux, C.; Geny, L.; Lapaillerie, D.; Claverol, S.; Bonneu, M.; Donèche, B. Proteome analysis of grape skins during ripening. J. Proteomics 2007, 58, 1851–1862. [Google Scholar]
- Deluc, L.G.; Grimplet, J.; Wheatley, M.D.; Tillett, R.L.; Quilici, D.R.; Osborne, C.; Schooley, D.A.; Schlauch, K.A.; Cushman, J.C.; Cramer, G.R. Transcriptomic and metabolite analyses of Cabernet Sauvignon grape berry development. BMC Genomics 2007, 8, 429. [Google Scholar] [CrossRef]
- Zhang, J.W.; Ma, H.Q.; Feng, J.D.; Zheng, L.; Wang, Z.; Chen, S.W. Grape berry plasma membrane proteome analysis and its differential expression during ripening. J. Exp. Bot. 2008, 59, 2979–2990. [Google Scholar] [CrossRef]
- Negri, A.S.; Prinsi, B.; Rossoni, M.; Failla, O.; Scienza, A.; Cocucci, M.; Espen, L. Proteome changes in the skin of the grape cultivar Barbera among different stages of ripening. BMC Genomics 2008, 8, 378. [Google Scholar]
- Lücker, J.; Laszczak, M.; Smith, D.; Lund, S.T. Generation of a predicted protein database from EST data and application to iTRAQ analyses in grape (Vitis vinifera cv. Cabernet Sauvignon) berries at ripening initiation. BMC Genomics 2009, 10, 50. [Google Scholar] [CrossRef]
- Zamboni, A.; Di Carli, M.; Guzzo, F.; Stocchero, M.; Zenoni, S.; Ferrarini, A.; Tononi, P.; Toffali, K.; Desiderio, A.; Lilley, K.S.; et al. Identification of putative stage-specific grapevine berry biomarkers and omics data integration into networks. Plant Physiol. 2010, 154, 1439–1459. [Google Scholar] [CrossRef]
- Martínez-Esteso, M.J.; Sellés-Marchart, S.; Lijavetzky, D.; Pedreño, M.A.; Bru-Martínez, R. A DIGE-based quantitative proteomic analysis of grape berry flesh development and ripening reveals key events in sugar and organic acid metabolism. J. Exp. Bot. 2011, 62, 2521–2569. [Google Scholar] [CrossRef]
- Bianco, L.; Lopez, L.; Scalone, A.G.; Di Carli, M.; Desiderio, A.; Benvenuto, E.; Perrotta, G. Strawberry proteome characterization and its regulation during fruit ripening and in different genotypes. J. Proteomics 2009, 72, 586–607. [Google Scholar] [CrossRef]
- Osorio, S.; Bombarely, A.; Giavalisco, P.; Usadel, B.; Stephens, C.; Araguez, I.; Medina-Escobar, N.; Botella, M.A.; Fernie, A.R.; Valpuesta, V. Demethylation of oligogalacturonides by FaPE1 in the fruits of the wild strawberry Fragaria vesca triggers metabolic and transcriptional changes associated with defence and development of the fruit. J. Exp. Bot. 2011, 62, 2855–2873. [Google Scholar]
- Katz, E.; Fon, M.; Eigenheer, R.A.; Phinney, B.S.; Fass, J.N.; Lin, D.W.; Sakda, A.; Blumwald, E. A label-free differential quantitative mass spectrometry method for the characterization and identification of protein changes during citrus fruit development. Proteome Sci. 2010, 8, 68. [Google Scholar] [CrossRef]
- Palma, J.M.; Corpas, F.J.; Del Río, L.A. Proteomics as an approach to the understanding of the molecular physiology of fruit development and ripening. J. Proteomics 2011, 74, 1230–1243. [Google Scholar] [CrossRef]
- Qin, G.; Meng, X.; Wang, Q.; Tian, S. Oxidative damage of mitochondrial proteins contributes to fruit senescence: A redox proteomics analysis. J. Proteome Res. 2009, 8, 2449–2462. [Google Scholar] [CrossRef]
- Jimenez, A.; Creissen, G.; Kular, B.; Firmin, J.; Robinson, S.; Verhoeyen, M.; Mullineaux, P. Changes in oxidative processes and components of the antioxidant system during tomato fruit ripening. Planta 2002, 214, 751–758. [Google Scholar] [CrossRef]
- Trainotti, L.; Bonghi, C.; Ziliotto, F.; Zanin, D.; Rasori, A.; Casadoro, G.; Ramina, A.; Tonutti, P. The use of microarray µPEACH1.0 to investigate transcriptome changes during transition from preclimacteric to climacteric phase in peach fruit. Plant Sci. 2006, 170, 606–813. [Google Scholar] [CrossRef]
- Kazt, E.; Fon, M.; Lee, Y.J.; Phinney, B.S.; Sadka, A.; Blumwald, E. The citrus fruit proteome: insights into the citrus fruit metabolism. Planta 2007, 226, 989–1005. [Google Scholar] [CrossRef]
- Muccilli, V.; Licciardello, C.; Fontanini, D.; Russo, M.P.; Cunsolo, V.; Saletti, R.; Recupero, G.R.; Foti, S. Proteome analysis of Citrus sinensis L. (Osbeck) flesh at ripening time. J. Proteomics 2009, 73, 134–152. [Google Scholar] [CrossRef]
- Wang, H.; Cao, G.; Prior, R.L. Total antioxidant capacity of fruits. J. Agric. Food Chem. 1996, 44, 701–705. [Google Scholar]
- Wolfe, K.L.; Kang, X.; He, X.; Dong, M.; Zhan, G.Q.; Liu, R.H. Cellular antioxidant activity of common fruits. J. Agric. Food Chem. 2008, 56, 8418–8426. [Google Scholar] [CrossRef]
- Fu, L.; Xu, B.T.; Xu, X.R.; Gan, R.Y.; Zhang, Y.; Xia, E.Q.; Li, H.B. Antioxidant capacities and total phenolic contents of 62 fruits. Food Chem. 2011, 129, 345–350. [Google Scholar] [CrossRef]
- Benzie, I.F.; Strain, J.J. The ferric reducing ability of plasma as a measure of “antioxidant power” the FRAP assay. Anal. Biochem. 1996, 239, 70–76. [Google Scholar] [CrossRef]
- Cao, G.; Prior, R.L. Measurement of oxygen radical absorbance capacity in biological samples. Methods Enzymol. 1999, 299, 50–62. [Google Scholar] [CrossRef]
- Evelson, P.; Travacio, M.; Repetto, M. Evaluation of total reactive antioxidant potential of tissue homogenates and their cytosols. Arch. Biochem. Biophys. 2001, 388, 261–266. [Google Scholar] [CrossRef]
- Ou, B.; Hampsch-Woodill, M.; Prior, R.L. Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. J. Agric. Food Chem. 2001, 49, 4619–4626. [Google Scholar] [CrossRef]
- Scalzo, J.; Politi, A.; Pellegrini, N.; Mezzetti, B.; Battino, M. Plant genotype affects total antioxidant capacity and phenolic contents in fruit. Nutrition 2005, 21, 207–213. [Google Scholar] [CrossRef]
- FAOSTAT. 2013. Available online: http://www.faostat.fao.org (accessed on 9 November 2013).
- Génard, M.; Lescourret, F.; Gómez, L.; Habib, R. Changes in fruit sugar concentrations in response to assimilate supply, metabolism and dilution: A modelling approach applied to peach fruit (Prunus persica). Tree Physiol. 2003, 23, 373–385. [Google Scholar] [CrossRef]
- Crisosto, C.H.; Crisosto, G.M. Relationship between ripe soluble solids concentration (RSSC) and consumer acceptance of high and low acid melting flesh peach and nectarine [Prunus persica (L.) Batsch] cultivars. Postharvest Biol. Technol. 2005, 38, 239–246. [Google Scholar] [CrossRef]
- Gil, M.I.; Tomas-Barberan, F.A.; Hess-Pierce, B.; Kader, A.A. Antioxidant capacities, phenolic compounds, carotenoids and vitamin C contents of nectarine, peach and plum cultivars from California. J. Agric. Food Chem. 2002, 50, 4976–4982. [Google Scholar] [CrossRef]
- Cantin, C.M.; Moreno, M.A.; Gogorcena, Y. Evaluation of the antioxidant capacity, phenolic compounds, and vitamin c content of different peach and nectarine [Prunus persica (L.) Batsch] Breeding Progenies. J. Agric. Food Chem. 2009, 57, 4586–4592. [Google Scholar] [CrossRef]
- Camejo, D.; Marti, M.C.; Roman, P.; Ortiz, A.; Jimenez, A. Antioxidant system and protein pattern in peach fruits at two maturation stages. J. Agric. Food Chem. 2010, 58, 11140–11147. [Google Scholar] [CrossRef]
- Legua, P.; Hernandez, F.; Huertas, M.; Diaz-Mula, M.; Valero, D.; Serrano, M. Quality, bioactive compounds, and antioxidant activity of new flat-type peach and nectarine cultivars: A comparative study. J. Food Sci. 2011, 76, C729–C735. [Google Scholar] [CrossRef]
- Abidi, W.; Jiménez, S.; Moreno, M.Á.; Gogorcena, Y. Evaluation of Antioxidant Compounds and Total Sugar Contentin a Nectarine [Prunus persica (L.) Batsch] Progeny. Int. J. Mol. Sci. 2011, 12, 6919–6935. [Google Scholar] [CrossRef]
- Montevecchi, G.; Vasile Simone, G.; Masino, F.; Bignami, C.; Antonelli, A. Physical and chemical characterization of Pescabivona, a Sicilian white flesh peach cultivar [Prunus persica (L.) Batsch]. Food Res. Int. 2012, 45, 123–131. [Google Scholar] [CrossRef]
- Tomàs-Barberàn, F.A.; Gil, M.I.; Cremin, P.; Waterhouse, A.L.; Hess-Pierce, B.; Kader, A.A. HPLC-DAD-ESIMS Analysis of phenolic compounds in nectarines, peaches, and plums. J. Agric. Food Chem. 2001, 49, 4748–4760. [Google Scholar]
- Cevallos-Casals, B.A.; Byrne, D.; Okie, W.R.; Cisneros-Zevallos, L. Selecting new peach and plum genotypes rich in phenolic compounds and enhanced functional properties. Food Chem. 2006, 96, 273–280. [Google Scholar] [CrossRef]
- Huang, R.H.; Xia, R.X.; Hu, L.M.; Lu, Y.M.; Wang, M.Y. Antioxidant activity and oxygen-scavenging system in orange pulp during fruit ripening and maturation. Sci. Hortic. 2007, 113, 166–172. [Google Scholar] [CrossRef]
- Chien, P.J.; Sheu, F.; Lin, H.R. Coating citrus (Murcott tangor) fruit with low molecular weight chitosan increases postharvest quality and shelf life. Food Chem. 2007, 100, 1160–1164. [Google Scholar] [CrossRef]
- Huang, R.H.; Liu, J.H.; Lu, Y.M.; Xia, R.X. Effect of salicylic acid on the antioxidant system in the pulp of ‘Cara Cara’ navel orange (Citrus sinensis L. Osbeck) at different storage temperatures. Postharvest Biol. Technol. 2008, 47, 168–175. [Google Scholar]
- Rapisarda, P.; Bianco, M.L.; Pannuzzo, P.; Timpanaro, N. Effect of cold storage on vitamin C, phenolics and antioxidant activity of five orange genotypes [Citrus sinensis (L.) Osbeck]. Postharvest Biol. Technol. 2008, 49, 348–354. [Google Scholar] [CrossRef]
- Alquezar, B.; Rodrigo, M.J.; Zacarías, L. Regulation of carotenoid biosynthesis during fruit maturation in the red-fleshed orange mutant Cara Cara. Phytochemistry 2008, 69, 1997–2007. [Google Scholar] [CrossRef]
- Proteggente, A.R.; Saija, A.; de Pasquale, A.; Rice-Evans, C.A. The compositional characterization and antioxidant activity of fresh juices from sicilian sweet Orange (Citrus sinensis L. Osbeck) varieties. Free Radic. Res. 2003, 37, 681–687. [Google Scholar] [CrossRef]
- Camarda, L.; di Stefano, V.; Fatta Del Bosco, S.; Schillaci, D. Antiproliferative activity of Citrus juices and HPLC evaluation of their flavonoid composition. Fitoterapia 2007, 78, 426–429. [Google Scholar] [CrossRef]
- Licciardello, C.; Russo, M.P.; Vale’, G.; Reforgiato, G.R. Identification of differentially expressed genes in the flesh of blood and common oranges. Tree Genet. Genomes 2008, 4, 315–331. [Google Scholar] [CrossRef]
- Maccarone, E.; Maccarrone, A.; Perrini, G.; Rapisarda, P. Anthocyanins of the Moro orange juice. Ann. Chim. (Rome) 1983, 73, 533–539. [Google Scholar]
- Maccarone, E.; Maccarrone, A.; Rapisarda, P. Acylated anthocyanins from oranges. Ann. Chim. (Rome) 1985, 75, 79–86. [Google Scholar]
- Rapisarda, P.; Bellomo, S.E.; Intrigliolo, F. Anthocyanins in Blood Oranges: Composition and Biological Activity. In Recent Research Developments in Agricultural and Food Chemistry; Pandalai, S.G., Ed.; Research Signpost: Trivandrum, India, 2001; pp. 217–230. [Google Scholar]
- Nagy, S. Vitamin C contents of Citrus fruit and their products: A review. J. Agric. Food Chem. 1980, 28, 8–18. [Google Scholar] [CrossRef]
- Vinson, J.A.; Bose, P. Comparative bioavailability to humans of ascorbic acid alone or in a citrus extract. Am. J. Clin. Nutr. 1988, 48, 601–604. [Google Scholar]
- Riso, P.; Visioli, F.; Gardana, C.; Grande, S.; Brusamolino, A.; Galvano, F.; Galvano, G.; Porrini, M. Effects of blood orange juice intake on antioxidant bioavailability and on different markers related to oxidative stress. J. Agric. Food Chem. 2005, 53, 941–947. [Google Scholar] [CrossRef]
- Rapisarda, P.; Intelisano, S. Sample preparation for vitamin C analysis of pigmented orange juice. Italian J. Food Sci. 1996, 8, 251–256. [Google Scholar]
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