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Appl. Sci. 2017, 7(5), 444; doi:10.3390/app7050444

Improved Imaging of Magnetically Labeled Cells Using Rotational Magnetomotive Optical Coherence Tomography

1
Anesthesiology and Intensive Care Medicine, Clinical Sensoring and Monitoring, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany
2
DFG-Center for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, 01307 Dresden, Germany
3
Department of Medical Physics and Biomedical Engineering, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany
4
Institute of Anatomy, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany
5
Institute for Metallic Materials, Leibniz Institute for Solid State and Materials Research, 01069 Dresden, Germany
6
German Center for Neurodegenerative Diseases (DZNE), 01307 Dresden, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Michael Pircher
Received: 20 January 2017 / Revised: 13 April 2017 / Accepted: 14 April 2017 / Published: 27 April 2017
(This article belongs to the Special Issue Development and Application of Optical Coherence Tomography (OCT))
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Abstract

In this paper, we present a reliable and robust method for magnetomotive optical coherence tomography (MM-OCT) imaging of single cells labeled with iron oxide particles. This method employs modulated longitudinal and transverse magnetic fields to evoke alignment and rotation of anisotropic magnetic structures in the sample volume. Experimental evidence suggests that magnetic particles assemble themselves in elongated chains when exposed to a permanent magnetic field. Magnetomotion in the intracellular space was detected and visualized by means of 3D OCT as well as laser speckle reflectometry as a 2D reference imaging method. Our experiments on mesenchymal stem cells embedded in agar scaffolds show that the magnetomotive signal in rotational MM-OCT is significantly increased by a factor of ~3 compared to previous pulsed MM-OCT, although the solenoid’s power consumption was 16 times lower. Finally, we use our novel method to image ARPE-19 cells, a human retinal pigment epithelium cell line. Our results permit magnetomotive imaging with higher sensitivity and the use of low power magnetic fields or larger working distances for future three-dimensional cell tracking in target tissues and organs. View Full-Text
Keywords: optical coherence tomography; cell imaging; dynamic contrast agents; magnetic particles; laser speckle; fluorescence microscopy optical coherence tomography; cell imaging; dynamic contrast agents; magnetic particles; laser speckle; fluorescence microscopy
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MDPI and ACS Style

Cimalla, P.; Walther, J.; Mueller, C.; Almedawar, S.; Rellinghaus, B.; Wittig, D.; Ader, M.; Karl, M.O.; Funk, R.H.W.; Brand, M.; Koch, E. Improved Imaging of Magnetically Labeled Cells Using Rotational Magnetomotive Optical Coherence Tomography. Appl. Sci. 2017, 7, 444.

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