Next Article in Journal
Previous Article in Journal
Previous Article in Special Issue
Pathogens 2013, 2(2), 193-208; doi:10.3390/pathogens2020193
Article

Use of a Th1 Stimulator Adjuvant for Vaccination against Neospora caninum Infection in the Pregnant Mouse Model

1,* , 2
, 2,3
 and 1,*
Received: 23 January 2013; in revised form: 13 February 2013 / Accepted: 19 March 2013 / Published: 27 March 2013
(This article belongs to the Special Issue Host-Parasite Interactions)
View Full-Text   |   Download PDF [383 KB, uploaded 27 March 2013]   |   Browse Figures
Abstract: Vertical transmission from an infected cow to its fetus accounts for the vast majority of new Neospora caninum infections in cattle. A vaccine composed of a chimeric antigen named recNcMIC3-1-R, based on predicted immunogenic domains of the two microneme proteins NcMIC1 and NcMIC3, the rhoptry protein NcROP2, and emulsified in saponin adjuvants, significantly reduced the cerebral infection in non-pregnant BALB/c mice. Protection was associated with a mixed Th1/Th2-type cytokine response. However, the same vaccine formulation elicited a Th2-type immune response in pregnant mice and did not prevent vertical transmission or disease, neither in dams nor in offspring mice. In this study, an alternative vaccine formulation containing recNcMIC3-1-R emulsified in Freund’s incomplete adjuvant, a stimulator of the cellular immunity, was investigated. No protection against vertical transmission and cerebral infection in the pregnant mice and a very limited protective effect in the non-pregnant mice were observed. The vaccine induced a Th1-type immune response characterized by high IgG2a titres and strong IFN-γ expression, which appeared detrimental to pregnancy.
Keywords: Neospora caninum; vaccination; recombinant antigen; pregnancy; vertical transmission; cytokines; mouse model; abortion Neospora caninum; vaccination; recombinant antigen; pregnancy; vertical transmission; cytokines; mouse model; abortion
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Monney, T.; Grandgirard, D.; Leib, S.L.; Hemphill, A. Use of a Th1 Stimulator Adjuvant for Vaccination against Neospora caninum Infection in the Pregnant Mouse Model. Pathogens 2013, 2, 193-208.

AMA Style

Monney T, Grandgirard D, Leib SL, Hemphill A. Use of a Th1 Stimulator Adjuvant for Vaccination against Neospora caninum Infection in the Pregnant Mouse Model. Pathogens. 2013; 2(2):193-208.

Chicago/Turabian Style

Monney, Thierry; Grandgirard, Denis; Leib, Stephen L.; Hemphill, Andrew. 2013. "Use of a Th1 Stimulator Adjuvant for Vaccination against Neospora caninum Infection in the Pregnant Mouse Model." Pathogens 2, no. 2: 193-208.

Pathogens EISSN 2076-0817 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert