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Insects 2017, 8(1), 32; doi:10.3390/insects8010032

Isolating Spermathecae and Determining Mating Status of Drosophila suzukii: A Protocol for Tissue Dissection and Its Applications

Department of Entomology, University of Wisconsin-Madison, 1630 Linden Drive Madison, WI 53706, USA
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Author to whom correspondence should be addressed.
Academic Editor: Mary L. Cornelius
Received: 11 November 2016 / Revised: 20 February 2017 / Accepted: 6 March 2017 / Published: 10 March 2017
(This article belongs to the Special Issue Invasive Insect Species)
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Abstract

The spotted wing drosophila, Drosophila suzukii (Diptera: Drosophilidae), is an emerging invasive pest, which attacks a wide variety of fruits and berries. Although previous studies have focused on different aspects of D. suzukii reproductive biology, there are no protocols available for determining the mating status of D. suzukii females and drosophilids in general. In this study, a step-by-step protocol for tissue dissection, isolating spermathecae, and determining the mating status of females was developed specifically for D. suzukii. This protocol is an effective and relatively quick method for determining female mating status. It has important applications from exploring reproductive output of D. suzukii females to understanding the biology of D. suzukii winter morph, which presumably plays the main role in the overwintering of this invasive species. We demonstrated applicability of this protocol for both field collected flies and flies reared in the lab, including fly specimens stored on a long-term basis. View Full-Text
Keywords: Drosophila suzukii; invasive species; mating status; spermathecae; sperm storage; spotted wing drosophila; tissue dissection Drosophila suzukii; invasive species; mating status; spermathecae; sperm storage; spotted wing drosophila; tissue dissection
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Avanesyan, A.; Jaffe, B.D.; Guédot, C. Isolating Spermathecae and Determining Mating Status of Drosophila suzukii: A Protocol for Tissue Dissection and Its Applications. Insects 2017, 8, 32.

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