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Insects 2017, 8(2), 55; doi:10.3390/insects8020055

A Transcriptome Survey Spanning Life Stages and Sexes of the Harlequin Bug, Murgantia histrionica

1
Invasive Insect Biocontrol and Behavior Laboratory, USDA-ARS, Beltsville, MD 20705, USA
2
Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN 38163, USA
3
Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Brian T. Forschler
Received: 27 March 2017 / Revised: 10 May 2017 / Accepted: 18 May 2017 / Published: 25 May 2017
(This article belongs to the Special Issue Invasive Insect Species)
View Full-Text   |   Download PDF [2630 KB, uploaded 25 May 2017]   |  

Abstract

The harlequin bug, Murgantia histrionica (Hahn), is an agricultural pest in the continental United States, particularly in southern states. Reliable gene sequence data are especially useful to the development of species-specific, environmentally friendly molecular biopesticides and effective biolures for this insect. Here, mRNAs were sampled from whole insects at the 2nd and 4th nymphal instars, as well as sexed adults, and sequenced using Illumina RNA-Seq technology. A global assembly of these data identified 72,540 putative unique transcripts bearing high levels of similarity to transcripts identified in other taxa, with over 99% of conserved single-copy orthologs among insects being detected. Gene ontology and protein family analyses were conducted to explore the functional potential of the harlequin bug’s gene repertoire, and phylogenetic analyses were conducted on gene families germane to xenobiotic detoxification, including glutathione S-transferases, carboxylesterases and cytochrome P450s. Genic content in harlequin bug was compared with that of the closely related invasive pest, the brown marmorated stink bug, Halyomorpha halys (Stål). Quantitative analyses of harlequin bug gene expression levels, experimentally validated using quantitative real-time PCR, identified genes differentially expressed between life stages and/or sexes. View Full-Text
Keywords: harlequin bug; invasive insects; agricultural pest; RNAi targets; insect pheromones; xenobiotic detoxification; transcriptomics harlequin bug; invasive insects; agricultural pest; RNAi targets; insect pheromones; xenobiotic detoxification; transcriptomics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Sparks, M.E.; Rhoades, J.H.; Nelson, D.R.; Kuhar, D.; Lancaster, J.; Lehner, B.; Tholl, D.; Weber, D.C.; Gundersen-Rindal, D.E. A Transcriptome Survey Spanning Life Stages and Sexes of the Harlequin Bug, Murgantia histrionica. Insects 2017, 8, 55.

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