Tissue-Specific Immune Gene Expression in the Migratory Locust, Locusta Migratoria
AbstractThe ability of hosts to respond to infection involves several complex immune recognition pathways. Broadly conserved pathogen-associated molecular patterns (PAMPs) allow individuals to target a range of invading microbes. Recently, studies on insect innate immunity have found evidence that a single pathogen can activate different immune pathways across species. In this study, expression changes in immune genes encoding peptidoglycan-recognition protein SA (PGRP-SA), gram-negative binding protein 1 (GNBP1) and prophenoloxidase (ProPO) were investigated in Locusta migratoria, following an immune challenge using injected lipopolysaccharide (LPS) solution from Escherichia coli. Since immune activation might also be tissue-specific, gene expression levels were followed across a range of tissue types. For PGRP-SA, expression increased in response to LPS within all seven of the tissue-types assayed and differed significantly between tissues. Expression of GNBP1 similarly varied across tissue types, yet showed no clear expression difference between LPS-injected and uninfected locusts. Increases in ProPO expression in response to LPS, however, could only be detected in the gut sections. This study has revealed tissue-specific immune response to add a new level of complexity to insect immune studies. In addition to variation in recognition pathways identified in previous works, tissue-specificity should be carefully considered in similar works. View Full-Text
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Pulpitel, T.; Pernice, M.; Simpson, S.J.; Ponton, F. Tissue-Specific Immune Gene Expression in the Migratory Locust, Locusta Migratoria. Insects 2015, 6, 368-380.
Pulpitel T, Pernice M, Simpson SJ, Ponton F. Tissue-Specific Immune Gene Expression in the Migratory Locust, Locusta Migratoria. Insects. 2015; 6(2):368-380.Chicago/Turabian Style
Pulpitel, Tamara; Pernice, Mathieu; Simpson, Stephen J.; Ponton, Fleur. 2015. "Tissue-Specific Immune Gene Expression in the Migratory Locust, Locusta Migratoria." Insects 6, no. 2: 368-380.