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Water 2017, 9(11), 824; doi:10.3390/w9110824

Recalcitrant Compounds Removal in Raw Leachate and Synthetic Effluents Using the White-Rot Fungus Bjerkandera adusta

Department of Biology, University of Pisa, Via Luca Ghini 13, 56126 Pisa, Italy
Department of Civil Engineering, University of Manitoba, 15 Gillson Street, R3T 5V6, Winnipeg, MB R3T 2N2, Canada
Department of Life Sciences and Systems Biology, University of Turin, viale Mattioli 25, 10125 Torino, Italy
Department of Civil and Environmental Engineering, University of Florence, Via S. Marta 3, 50139 Florence, Italy
BD Biodigressioni S.r.l., Lungarno Mediceo, 56127 Pisa, Italy
Author to whom correspondence should be addressed.
Received: 6 September 2017 / Revised: 13 October 2017 / Accepted: 24 October 2017 / Published: 27 October 2017
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Recalcitrant compounds limit the efficiency of conventional biological processes for wastewater treatment, representing one of the major issues in the field. This study focused on the treatment of three effluents with White-Rot-Fungus (WRF) Bjerkandera adusta MUT 2295 in batch tests, with biomass cultivated in attached form on polyurethane foam cubes (PUFs) to test its efficiency in the removal of the target effluents’ recalcitrant fraction. Treatment efficiency of B. adusta was evaluated on landfill leachate (Canada) and two solutions containing synthetic recalcitrant compounds, which were prepared with tannic and humic acid. Chemical Oxygen Demand (COD) and color removal, the production of manganese peroxidases, and the consumption of a co-substrate (glucose) were monitored during the experiment. Biological Oxygen Demand (BOD5) and fungal dry weight were measured at the beginning and at the end of the experiment. After co-substrate addition, effluent COD was 2300 ± 85, 2545 ± 84, and 2580 ± 95 (mg/L) in raw leachate and tannic and humic acids, respectively. COD removal of 48%, 61%, and 48% was obtained in raw leachate and in the synthetic effluents containing tannic and humic acids, respectively. Color removal of 49%, 25%, and 42% was detected in raw leachate and in tannic and humic acid solutions, respectively. COD and color removals were associated with the increase of fungal dry weight, which was observed in all the trials. These results encourage the use of the selected fungal strain to remove tannic acid, while further investigations are required to optimize leachate and humic acid bioremediation. View Full-Text
Keywords: bioremediation; landfill leachate; recalcitrant compounds; wastewater treatment; white-rot fungi bioremediation; landfill leachate; recalcitrant compounds; wastewater treatment; white-rot fungi

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Bardi, A.; Yuan, Q.; Tigini, V.; Spina, F.; Varese, G.C.; Spennati, F.; Becarelli, S.; Di Gregorio, S.; Petroni, G.; Munz, G. Recalcitrant Compounds Removal in Raw Leachate and Synthetic Effluents Using the White-Rot Fungus Bjerkandera adusta. Water 2017, 9, 824.

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