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Genes 2017, 8(1), 1; doi:10.3390/genes8010001

A Spatial Control for Correct Timing of Gene Expression during the Escherichia coli Cell Cycle

1,†
,
1,†
,
1,2
,
3
and
1,*
1
School of Life Sciences, Inner Mongolia University, Hohhot 010021, China
2
School of Life Sciences, Arizona State University, Tempe, AZ 85287-4501, USA
3
Department of Public Health Sciences, Karolinska Institutet, SE-171 77 Stockholm, Sweden
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Paolo Cinelli
Received: 26 August 2016 / Revised: 9 December 2016 / Accepted: 15 December 2016 / Published: 23 December 2016
(This article belongs to the Section Molecular Genetics)
View Full-Text   |   Download PDF [3123 KB, uploaded 23 December 2016]   |  

Abstract

Temporal transcriptions of genes are achieved by different mechanisms such as dynamic interaction of activator and repressor proteins with promoters, and accumulation and/or degradation of key regulators as a function of cell cycle. We find that the TorR protein localizes to the old poles of the Escherichia coli cells, forming a functional focus. The TorR focus co-localizes with the nucleoid in a cell-cycle-dependent manner, and consequently regulates transcription of a number of genes. Formation of one TorR focus at the old poles of cells requires interaction with the MreB and DnaK proteins, and ATP, suggesting that TorR delivery requires cytoskeleton organization and ATP. Further, absence of the protein–protein interactions and ATP leads to loss in function of TorR as a transcription factor. We propose a mechanism for timing of cell-cycle-dependent gene transcription, where a transcription factor interacts with its target genes during a specific period of the cell cycle by limiting its own spatial distribution. View Full-Text
Keywords: cell-cycle-dependent; co-localization; TorR focus; nucleoid; spatial control; gene expression cell-cycle-dependent; co-localization; TorR focus; nucleoid; spatial control; gene expression
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Yao, Y.; Fan, L.; Shi, Y.; Odsbu, I.; Morigen. A Spatial Control for Correct Timing of Gene Expression during the Escherichia coli Cell Cycle. Genes 2017, 8, 1.

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