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Genes 2017, 8(1), 7; doi:10.3390/genes8010007

Parameters for Successful Parental RNAi as An Insect Pest Management Tool in Western Corn Rootworm, Diabrotica virgifera virgifera

1
Department of Entomology, University of Nebraska, 103 Entomology Hall, Lincoln, NE 68583, USA
2
Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN 46268, USA
3
Entomology and Nematology Department, University of Florida, Charles Steinmetz Hall, PO Box 110620, Gainesville, FL 32611, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Wenyi Gu
Received: 12 November 2016 / Revised: 17 December 2016 / Accepted: 19 December 2016 / Published: 24 December 2016
(This article belongs to the Special Issue RNA Interference 2016)
View Full-Text   |   Download PDF [1901 KB, uploaded 24 December 2016]   |  

Abstract

Parental RNAi (pRNAi) is an RNA interference response where the gene knockdown phenotype is observed in the progeny of the treated organism. pRNAi has been demonstrated in female western corn rootworms (WCR) via diet applications and has been described as a potential approach for rootworm pest management. However, it is not clear if plant-expressed pRNAi can provide effective control of next generation WCR larvae in the field. In this study, we evaluated parameters required to generate a successful pRNAi response in WCR for the genes brahma and hunchback. The parameters tested included a concentration response, duration of the dsRNA exposure, timing of the dsRNA exposure with respect to the mating status in WCR females, and the effects of pRNAi on males. Results indicate that all of the above parameters affect the strength of pRNAi phenotype in females. Results are interpreted in terms of how this technology will perform in the field and the potential role for pRNAi in pest and resistance management strategies. More broadly, the described approaches enable examination of the dynamics of RNAi response in insects beyond pRNAi and crop pests. View Full-Text
Keywords: rootworm; Diabrotica; RNAi; parental RNAi; insect resistance; brahma; hunchback; chromatin-remodeling ATPase; transgenic crops rootworm; Diabrotica; RNAi; parental RNAi; insect resistance; brahma; hunchback; chromatin-remodeling ATPase; transgenic crops
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Vélez, A.M.; Fishilevich, E.; Matz, N.; Storer, N.P.; Narva, K.E.; Siegfried, B.D. Parameters for Successful Parental RNAi as An Insect Pest Management Tool in Western Corn Rootworm, Diabrotica virgifera virgifera. Genes 2017, 8, 7.

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