Genes 2012, 3(4), 702-741; doi:10.3390/genes3040702
Review

RNAi in Arthropods: Insight into the Machinery and Applications for Understanding the Pathogen-Vector Interface

1 Department of Biochemistry, University of Pretoria, Pretoria, 0002, South Africa 2 Institut für Parasitologie und Tropenveterinärmedizin, Freie Universität Berlin, Königsweg 67, 14163, Berlin, Germany 3 Department of Genetics, University of Pretoria, Pretoria, 0002, South Africa
* Author to whom correspondence should be addressed.
Received: 3 September 2012; in revised form: 19 October 2012 / Accepted: 23 October 2012 / Published: 6 November 2012
(This article belongs to the Special Issue RNA Interference)
PDF Full-text Download PDF Full-Text [686 KB, uploaded 6 November 2012 15:44 CET]
Abstract: The availability of genome sequencing data in combination with knowledge of expressed genes via transcriptome and proteome data has greatly advanced our understanding of arthropod vectors of disease. Not only have we gained insight into vector biology, but also into their respective vector-pathogen interactions. By combining the strengths of postgenomic databases and reverse genetic approaches such as RNAi, the numbers of available drug and vaccine targets, as well as number of transgenes for subsequent transgenic or paratransgenic approaches, have expanded. These are now paving the way for in-field control strategies of vectors and their pathogens. Basic scientific questions, such as understanding the basic components of the vector RNAi machinery, is vital, as this allows for the transfer of basic RNAi machinery components into RNAi-deficient vectors, thereby expanding the genetic toolbox of these RNAi-deficient vectors and pathogens. In this review, we focus on the current knowledge of arthropod vector RNAi machinery and the impact of RNAi on understanding vector biology and vector-pathogen interactions for which vector genomic data is available on VectorBase.
Keywords: RNA interference; vector; disease; mosquito; ixodid ticks; body louse; kissing bug; tsetse fly; transgenesis; vaccine; drug target

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Barnard, A.-C.; Nijhof, A.M.; Fick, W.; Stutzer, C.; Maritz-Olivier, C. RNAi in Arthropods: Insight into the Machinery and Applications for Understanding the Pathogen-Vector Interface. Genes 2012, 3, 702-741.

AMA Style

Barnard A-C, Nijhof AM, Fick W, Stutzer C, Maritz-Olivier C. RNAi in Arthropods: Insight into the Machinery and Applications for Understanding the Pathogen-Vector Interface. Genes. 2012; 3(4):702-741.

Chicago/Turabian Style

Barnard, Annette-Christi; Nijhof, Ard M.; Fick, Wilma; Stutzer, Christian; Maritz-Olivier, Christine. 2012. "RNAi in Arthropods: Insight into the Machinery and Applications for Understanding the Pathogen-Vector Interface." Genes 3, no. 4: 702-741.

Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert