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Size Polymorphism in Alleles of the Myoglobin Gene from Biomphalaria Mollusks
Department of Biochemistry and Immunology, Federal University of Minas Gerais, Belo Horizonte/MG, 31270-901, Brazil
Department of Zoology, Federal University of Minas Gerais, Belo Horizonte/MG, 31270-901, Brazil
Research Center René Rachou, Belo Horizonte/MG, 30190-002, Brazil
Department of Physiological Sciences, Federal University of Espírito Santo, Vitria/ES, 29075-91, Brazil
* Author to whom correspondence should be addressed.
Received: 8 September 2010; in revised form: 7 October 2010 / Accepted: 14 October 2010 / Published: 20 October 2010
Abstract: Introns are common among all eukaryotes, while only a limited number of introns are found in prokaryotes. Globin and globin-like proteins are widely distributed in nature, being found even in prokaryotes and a wide range of patterns of intron-exon have been reported in several eukaryotic globin genes. Globin genes in invertebrates show considerable variation in the positions of introns; globins can be found without introns, with only one intron or with three introns in different positions. In this work we analyzed the introns in the myoglobin gene from Biomphalaria glabrata, B. straminea and B. tenagophila. In the Biomphalaria genus, the myoglobin gene has three introns; these were amplified by PCR and analyzed by PCR-RFLP. Results showed that the size (number or nucleotides) and the nucleotide sequence of the coding gene of the myoglobin are variable in the three species. We observed the presence of size polymorphisms in intron 2 and 3; this characterizes a homozygous/heterozygous profile and it indicates the existence of two alleles which are different in size in each species of Biomphalaria. This polymorphism could be explored for specific identification of Biomphalaria individuals.
Keywords: Biomphalaria; myoglobin gene; size polymorphism
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Teixeira, K.N.; Souza, K.N.; Vidigal, T.H.; Brito, C.A.; Santos, A.M.; Santoro, M.M. Size Polymorphism in Alleles of the Myoglobin Gene from Biomphalaria Mollusks. Genes 2010, 1, 357-370.
Teixeira KN, Souza KN, Vidigal TH, Brito CA, Santos AM, Santoro MM. Size Polymorphism in Alleles of the Myoglobin Gene from Biomphalaria Mollusks. Genes. 2010; 1(3):357-370.
Teixeira, Kádima N.; Souza, Karyne N.; Vidigal, Teofânia H.D.A.; Brito, Cristiane A.; Santos, Alexandre M.C.; Santoro, Marcelo M. 2010. "Size Polymorphism in Alleles of the Myoglobin Gene from Biomphalaria Mollusks." Genes 1, no. 3: 357-370.