Genes 2010, 1(3), 388-412; doi:10.3390/genes1030388
Article

Establishment and Molecular Cytogenetic Characterization of a Cell Culture Model of Head and Neck Squamous Cell Carcinoma (HNSCC)

1 Department of Radiation Cytogenetics, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstr.1, 85764 Neuherberg, Germany 2 Institute of Pathology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstr.1, 85764 Neuherberg, Germany
* Author to whom correspondence should be addressed.
Received: 17 September 2010; in revised form: 30 September 2010 / Accepted: 28 October 2010 / Published: 11 November 2010
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Abstract: Cytogenetic analysis of head and neck squamous cell carcinoma (HNSCC) established several biomarkers that have been correlated to clinical parameters during the past years. Adequate cell culture model systems are required for functional studies investigating those potential prognostic markers in HNSCC. We have used a cell line, CAL 33, for the establishment of a cell culture model in order to perform functional analyses of interesting candidate genes and proteins. The cell line was cytogenetically characterized using array CGH, spectral karyotyping (SKY) and fluorescence in situ hybridization (FISH). As a starting point for the investigation of genetic markers predicting radiosensitivity in tumor cells, irradiation experiments were carried out and radiation responses of CAL 33 have been determined. Radiosensitivity of CAL 33 cells was intermediate when compared to published data on tumor cell lines.
Keywords: HNSCC; CAL 33; cell lines; cytogenetics

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MDPI and ACS Style

Bauer, V.L.; Hieber, L.; Schaeffner, Q.; Weber, J.; Braselmann, H.; Huber, R.; Walch, A.; Zitzelsberger, H. Establishment and Molecular Cytogenetic Characterization of a Cell Culture Model of Head and Neck Squamous Cell Carcinoma (HNSCC). Genes 2010, 1, 388-412.

AMA Style

Bauer VL, Hieber L, Schaeffner Q, Weber J, Braselmann H, Huber R, Walch A, Zitzelsberger H. Establishment and Molecular Cytogenetic Characterization of a Cell Culture Model of Head and Neck Squamous Cell Carcinoma (HNSCC). Genes. 2010; 1(3):388-412.

Chicago/Turabian Style

Bauer, Verena L.; Hieber, Ludwig; Schaeffner, Quirin; Weber, Johannes; Braselmann, Herbert; Huber, Reinhard; Walch, Axel; Zitzelsberger, Horst. 2010. "Establishment and Molecular Cytogenetic Characterization of a Cell Culture Model of Head and Neck Squamous Cell Carcinoma (HNSCC)." Genes 1, no. 3: 388-412.

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