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Polymers 2016, 8(8), 280; doi:10.3390/polym8080280

Polymerization of Various Lignins via Immobilized Myceliophthora thermophila Laccase (MtL)

1
Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences, Vienna, Konrad Lorenz Str. 20, 3430 Tulln, Austria
2
Department of Biology, Botswana International University of Science and Technology, Private Bag 16, Plot 10017 Palapye, Botswana
3
Austrian Centre of Industrial Biotechnology ACIB GmbH, Konrad Lorenz Str. 20, 3430 Tulln, Austria
*
Author to whom correspondence should be addressed.
Academic Editor: Katja Loos
Received: 27 June 2016 / Revised: 25 July 2016 / Accepted: 29 July 2016 / Published: 3 August 2016
(This article belongs to the Special Issue Enzymatic Polymer Synthesis)
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Abstract

Enzymatic polymerization of lignin is an environmentally-friendly and sustainable method that is investigated for its potential in opening-up new applications of one of the most abundant biopolymers on our planet. In this work, the laccase from Myceliophthora thermophila was successfully immobilized onto Accurel MP1000 beads (67% of protein bound to the polymeric carrier) and the biocatalyzed oxidation of Kraft lignin (KL) and lignosulfonate (LS) were carried out. Fluorescence intensity determination, phenol content analysis and size exclusion chromatography were performed in order to elucidate the extent of the polymerization reaction. The collected results show an 8.5-fold decrease of the LS samples’ fluorescence intensity after laccase-mediated oxidation and a 12-fold increase of the weight average molecular weight was obtained. View Full-Text
Keywords: lignin; biocatalyzed polymerization; enzyme immobilization; laccase; green processing lignin; biocatalyzed polymerization; enzyme immobilization; laccase; green processing
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Huber, D.; Pellis, A.; Daxbacher, A.; Nyanhongo, G.S.; Guebitz, G.M. Polymerization of Various Lignins via Immobilized Myceliophthora thermophila Laccase (MtL). Polymers 2016, 8, 280.

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